FastQCFastQC Report
Wed 30 Apr 2025
1C1-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1C1-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9730281
Total Bases230.8 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA6763196.950662575931774No Hit
CTCGGGCTGAGACTTGAAGCG5527055.680257332753288No Hit
CGAGTGTGCTCTTAACTG2040122.096671206103914No Hit
GTTGGATCCGGCCCGGACTGGCC1869911.9217430616854745No Hit
CTCGGGCTGAGACTTGAAGCGGTT1553871.5969425754508013No Hit
CTCGGGCTGAGACTTGAAGC1337551.3746262826325364No Hit
CAGCTGTGACCCTCGGGT1285521.3211540345032173No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA1127041.1582810403933865No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC1065601.0951379513089088No Hit
CAGCTGTGACCCTCGGGTC862410.8863156161677139No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAT756310.7772745720293175No Hit
GAGTGTGCTCTTAACTGA699400.7187870525013615No Hit
CGACTGCTGCCGGTCGTTGGGGCG689330.7084379166439283No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC654580.6727246623196185No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT605440.6222225236866232No Hit
CGTGGTGGACTGCTTGCAGTGCT597380.6139391041224812No Hit
GAGACCGAGTGTGCTCTTAACTG496660.5104271911571722No Hit
GAGGTGTAGAATAAGTGGGAGCAGCT494240.5079401098488318No Hit
GACCAAGATAGTCGAACTCGACCCTGTC485420.4988756234275249No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCC463730.47658438641186207No Hit
GAGGTGTAGAATAAGTGGGAGCAGCTCGC456980.4696472794567803No Hit
GAGACCGAGTGTGCTCTTAACT438380.4505316958472217No Hit
CCGAGCTGTTCTTCCTCGCCGAGACC397660.40868295581597286No Hit
CTCGGGCTGAGACTTGAAGCA391950.4028146771917481No Hit
CGGACTGGCCGTGGTCGAGGT356310.36618675246891635No Hit
GGAAGGAATCGGCCGTGGATTGGCC341470.3509353943632255No Hit
TCTCGGGCTGAGACTTGAAGCG339870.3492910430849839No Hit
AGACTTGAAGCGGTTGGAT323830.3328064215206118No Hit
CTCGGGCTGAGACTTGAAGCGG299200.3074936890311801No Hit
TTCCGAGGTCGCGGTCGGACCG296930.3051607656551748No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCA294820.3029922774069937No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC281040.28883030202313786No Hit
GAGTGTGCTCTTAACTG278140.285849915331325No Hit
CTCGGGCTGAGACTTGAAGCGGT276890.2845652658951987No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTC255180.2622534744885579No Hit
GAGACTTGAAGCGGTTGGA248850.25574800974401457No Hit
GAGACTTGAAGCGGTTGGAT244120.2508868962777128No Hit
GTTCTATTTTGTTGGTTTCTGGAACC236180.2427268030594389No Hit
CCGAGCTGTTCTTCCTCGCCGAGAC233530.24000334625485123No Hit
CGGACTGGCCGTGGTCGAGGC229830.23620078392391752No Hit
AGGAATCGGCCGTGGATTGGCC229600.2359644084276703No Hit
CGAGTGTGCTCTTAACTGA224530.23075387031474218No Hit
GATCCGGCCCGGACTGGCC218230.2242792371566659No Hit
GAGTGTGCTCTTAACT217550.22358038786341317No Hit
CACTACCGATAGCCAACTCGAACGTTTC214930.22088776264529258No Hit
CACTTTCTGGAGTTAAGGCT211380.217239358246694No Hit
GCCGAGCTGTTCTTCCTCGCCGAGACC191750.1970652235017673No Hit
TGGGGGTAGTCGTTCTATGT191610.19692134276492115No Hit
GGATCCGGCCCGGACTGGCC189300.19454731060695987No Hit
CGACTGCTGCCGGTCGTTGGGGC188830.19406428241897639No Hit
GACTTGAAGCGGTTGGAT175990.1808683634110875No Hit
GAATCGGCCGTGGATTGGC168760.17343795107253326No Hit
GAAGGAATCGGCCGTGGATTGGCC162720.1672305249971712No Hit
GTTGGATCCGGCCCGGACTGGCT162290.16678860559114378No Hit
CTTTCTGGAGTTAAGGCT158800.16320186436547926No Hit
AGTGTGCTCTTAACTG149240.15337686547798568No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA148990.15311993559076045No Hit
GTTGGATCCGGCCCGGACTGGC148870.1529966092448923No Hit
CGGACTGGCCGTGGTCGAGG148690.15281161972609014No Hit
AGACTTGAAGCGGTTGGA147010.15108505088393645No Hit
GCCGAGCTGTTCTTCCTCGCCGAGAC146440.15049925074106288No Hit
TGAAAATCTTTGCTCTGAAGTGGA144680.1486904643349971No Hit
GCACTACCGATAGCCAACTCGAACGTTTC144610.14861852396657405No Hit
CTTTCCGAGGTCGCGGTC142910.14687140073344232No Hit
AGTGTGCTCTTAACT142470.1464192041319259No Hit
CTCGGGCTGAGACTTGAAGCGGTTG140750.14465152650781618No Hit
TTCCGAGGTCGCGGTCGGACCGGGAAG133090.1367791947632345No Hit
TCACTTTCTGGAGTTAAGGCT130920.1345490433421193No Hit
GGGGGTATAGCTCAGTGGTAGAGCATT128430.1319900216653558No Hit
GGAATCGGCCGTGGATTGGCC123760.1271905713719881No Hit
AGTGTGCTCTTAACTGAG123660.12708779941709802No Hit
CGACCAAGATAGTCGAACTCGACCCTGTT123270.12668698879302664No Hit
TCTCGGGCTGAGACTTGAAGCGGTT121920.1252995674020103No Hit
TTGGATCCGGCCCGGACTGGCC121640.125011805928318No Hit
CACTACCGATAGCCAACTCGAACGTTTCCG121520.12488847958244988No Hit
CGGACTGGCCGTGGTCGAGGTGA119990.12331606867263134No Hit
CGACTGCTGCCGGTCGTTGGGGCGGT118180.12145589628912053No Hit
GACCAAGATAGTCGAACTCGAC117180.12042817674021954No Hit
GAGACCGAGTGTGCTCTTAACTGA113450.1165947828228188No Hit
AGACCGAGTGTGCTCTTAACT111220.11430296822876956No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA103460.10632786452929777No Hit
GACTGCTGCCGGTCGTTGGGGCG101360.10416965347660567No Hit
CGTGGTGGACTGCTTGCAGT100710.10350163576982No Hit
GAGGTGTAGAATAAGTGGGAGCAGC99320.10207310559684761No Hit
TTCCGAGGTCGCGGTCGGACCGGGA97420.1001204384539357No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)