FastQCFastQC Report
Wed 30 Apr 2025
1B8-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1B8-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5509163
Total Bases136 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGGCTGAGACTTGAAGCG2482464.506056546157738No Hit
TGAAAATCTTTGCTCTGAAGTGGAA2354084.273026592242778No Hit
CGGACTGGCCGTGGTCGAGGC1953993.5468001219059957No Hit
GTTGGATCCGGCCCGGACTGGCC1908733.4646460814464923No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA1346432.443982869993137No Hit
TGAAAATCTTTGCTCTGAGGTGGAA1322792.4010725404203868No Hit
CTCGGGCTGAGACTTGAAGCGGTT1239182.249307199659912No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC973991.7679455118681366No Hit
TGAAAATCTTTACTCTGAAGTGGAA819041.4866868161279672No Hit
CAGCTGTGACCCTCGGGTC715311.2984005011287558No Hit
CGTGGTGGACTGCTTGCAGTGCT682591.2390085390466754No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAT615221.1167213603953996No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC486170.882475250777659No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT370830.6731149541227951No Hit
CTCGGGCTGAGACTTGAAGC315880.5733720349171009No Hit
CAGCTGTGACCCTCGGGT314270.5704496309148958No Hit
GGACTGGCCGTGGTCGAGGC287150.521222552318746No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCC281520.511003214099855No Hit
CTCGGGCTGAGACTTGAAGCA255920.4645351753070294No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA248480.45103040153286444No Hit
CGGACTGGCCGTGGTCGAGGT221110.4013495334953785No Hit
GTTGGATCCGGCCCGGACTGGC213690.3878810628765204No Hit
GTTGGATCCGGCCCGGACTGGCT213040.38670121032904636No Hit
ACCCGTAGATCCGAACTTGTGG207090.3759010216252451No Hit
TTACCGTGACCGATCCCGT204590.3713631272118832No Hit
CTCGGGCTGAGACTTGAAGCGG179300.32545778732631436No Hit
CTCGGGCTGAGACTTGAAGCGGTTG177840.322807656988911No Hit
GGAAGGAATCGGCCGTGGATTGGCC162680.29528986526628453No Hit
GAGACCGAGTGTGCTCTTAACTG161200.29260343177357434No Hit
TCTCGGGCTGAGACTTGAAGCGGTTGGA142120.2579702216107964No Hit
GAGACTTGAAGCGGTTGGA139080.25245214200414834No Hit
GAGACTTGAAGCGGTTGGAT136790.24829543072150886No Hit
CTTTCCGAGGTCGCGGTC132730.24092589019420918No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGA121340.22025124324693246No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC121200.21999712115978415No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA119420.21676614033747052No Hit
CTCGGGCTGAGACTTGAAGCGGT117840.21389819106822577No Hit
TATTAATGCTGTAGGTGAAAGACA116680.2117926080604259No Hit
CGGTTAGGCGAACAAGAATAGAGGC113800.20656495369623298No Hit
TCTCGGGCTGAGACTTGAAGCG113310.20567552639121403No Hit
TCTCGGGCTGAGACTTGAAGCGGTT113110.2053124948381451No Hit
AGACTTGAAGCGGTTGGAT108380.1967267986080644No Hit
CGGTTAGGCGAACAAGAATAGAGGCC106650.193586575674018No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG99740.18104383551548575No Hit
CGAGTGTGCTCTTAACTG96520.17519902751107563No Hit
GTTGGATCCGGCCCGGACT94940.17233107824183092No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT92560.16801100276031042No Hit
GACTGGCCGTGGTCGAGGC90830.164870779826264No Hit
ACGGTTAGGCGAACAAGAATAGAGGCC88810.1612041611402676No Hit
GTTGGATCCGGCCCGGACTGGCA87930.1596068223067642No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCA82800.15029506297054562No Hit
CTCGGGCTGAGACTTGAAGCGGTTGG82590.1499138798398232No Hit
CACCCCTCATCCGCTACTTTGACCTCATT82460.1496779093303284No Hit
GAGACCGAGTGTGCTCTTAACT79570.14443210338848206No Hit
GGATCCGGCCCGGACTGGCC78250.142036095138227No Hit
TTGGATCCGGCCCGGACTGGCC78060.14169121516281147No Hit
ACCCGTAGATCCGAACTTGT77680.1410014552119805No Hit
AGACTTGAAGCGGTTGGATCCGGCCC76490.1388414174712202No Hit
CGACTGCTGCCGGTCGTTGGGGC75480.137008108128222No Hit
TGGGGGTAGTCGTTCTATGT72090.1308547233037033No Hit
GACCGTGGTGGACTGCTTGCAGTGCT71170.12918477815958612No Hit
GAGACTTGAAGCGGTTGGATCCGGCCC69480.1261171615361535No Hit
TTCTGCTGAACTACTTTGAGAACGAGT68550.12442906481438286No Hit
CTCGGGCTGAGACTTGAAGCGGTC66710.12108917452614854No Hit
CTTTCCGAGGTCGCGGTCGGA65970.11974595777979341No Hit
CCCCGCCCTTTGACCTCATT65000.11798525474740901No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA64260.11664203800105387No Hit
GGTTGGATCCGGCCCGGACTGGC63570.11538957914296599No Hit
CCGCGACTGCTGCCGGTCGTTGGGGCGGT63420.11511730547816429No Hit
CGTGGTGGACTGCTTGCAGTGCC63060.11446384868264017No Hit
CGGACTGGCCGTGGTCGAGGCG62640.11370148242119539No Hit
AGACTTGAAGCGGTTGGATCCGGCC61150.1109968973508317No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC58450.10609597138440087No Hit
CGGACTGGCCGTGGTCGAGGCGAG57950.10518839250172847No Hit
GATCCGGCCCGGACTGGCC57600.10455308728385782No Hit
CTTTCCGAGGTCGCGGTCGGACCG56150.10192110852410793No Hit
GAGGTCCGGACGGCTGAGGGTTATCTCACA55770.10123134857327692No Hit
GAGACTTGAAGCGGTTGGATCCGGCC55590.10090462017551485No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)