FastQCFastQC Report
Wed 30 Apr 2025
1A6-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1A6-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9632727
Total Bases237.7 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA115967512.038906531867871No Hit
CTCGGGCTGAGACTTGAAGCG1589811.6504256790418745No Hit
CGGACTGGCCGTGGTCGAGGC1355901.407597246345713No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC1316961.3671725566394646No Hit
CGTGGTGGACTGCTTGCAGTGCT1089851.131403391791338No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC950450.9866884009066176No Hit
CAGCTGTGACCCTCGGGTC809000.8398452483912395No Hit
CGACTGCTGCCGGTCGTTGGGGC678030.7038816733828333No Hit
CTCGGGCTGAGACTTGAAGCGGTT676320.7021064751445774No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA623260.6470234233774091No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT487090.5056615847205054No Hit
CTCGGGCTGAGACTTGAAGC469290.48718291300064875No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAT444580.46153077939403864No Hit
CAGCTGTGACCCTCGGGT383630.3982569006679002No Hit
GAGACCGAGTGTGCTCTTAACTG375860.39019064902389533No Hit
GTTGGATCCGGCCCGGACTGGCC362000.37580220014540017No Hit
CGTAGTTGGATTTCGGGATGGCCCG321220.33346735560968355No Hit
CTGACTGGCGCCGACACT312860.32478860866709913No Hit
TGAAAATCTTTGCTCTGAAGTGGA276290.28682428143141603No Hit
GAGACCGAGTGTGCTCTTAACT262060.2720517253317778No Hit
CGGACTGGCCGTGGTCGAGGT258090.2679303586616749No Hit
CTCGGGCTGAGACTTGAAGCA255840.2655945715060751No Hit
CTGACTGGCGCCGACACTCTGTCTCGTGCA252480.26210646268704596No Hit
CGAGTGTGCTCTTAACTG251510.26109947889107626No Hit
CCGAGCTGTTCTTCCTCGCCGAGACC241800.25101925965513194No Hit
ACGATCTTCTGAGATTAAGCCTTTGTT241020.25020952010785735No Hit
CTTTCCGAGGTCGCGGTC240560.24973198140049022No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT240240.24939978056058268No Hit
GGACTGGCCGTGGTCGAGGC236140.2451434572992674No Hit
CGACTGCTGCCGGTCGTTGGGGCG235720.24470744369688874No Hit
CGCCGAGACCGAGTGTGCT219680.22805587659652352No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC213810.2219620674394696No Hit
GAGACTTGAAGCGGTTGGAT212900.2210173713009826No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA207180.21507928128763537No Hit
TGAAAATCTTTGCTCTGAAGTGGAT205700.213542852403063No Hit
CGACTGCTGCCGGTCGTTGGGGCGGT201810.20950453594293703No Hit
GCCGAGCTGTTCTTCCTCGCCGAGACC193080.20044168177920954No Hit
CTGACTGGCGCCGACACTCT189340.19655908446279025No Hit
GAATCGGCCGTGGATTGGC183520.19051718168697193No Hit
TGAAAATCTTTGCTCTGAAGTGG181760.18869007706748048No Hit
CCGCGACTGCTGCCGGTCGTTGGGGCGGT172330.17890053356645527No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC160540.166661008871112No Hit
AGACTTGAAGCGGTTGGAT159840.16593431953381427No Hit
CTCGGGCTGAGACTTGAAGCGG159510.16559173741765962No Hit
ACGATCTTCTGAGATTAAGCCTTTGT159290.16536334934022318No Hit
TTCTGCTGAACTACTTTGAGAACGAGT157960.16398263959935747No Hit
GAGACTTGAAGCGGTTGGA156150.16210362859863048No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC154700.16059834354279945No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG153810.15967440995680662No Hit
GACCGTGGTGGACTGCTTGCAGTGCT149870.15558418711544508No Hit
GACTGCTGCCGGTCGTTGGGGC148360.15401661440213138No Hit
CGGTTAGGCGAACAAGAATAGAGGC143810.14929313370969613No Hit
CGACTGCTGCCGGTCGTTGGGGT141410.1468016274103896No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCC141270.14665628954293006No Hit
ACTGCTGCCGGTCGTTGGGGC138960.14425821472984754No Hit
CGGTTAGGCGAACAAGAATAGAGGCC138400.14367686326000934No Hit
TTGTGTAACTCCCTAAGGAAGG134370.1394932089324238No Hit
CGTGGTGGACTGCTTGCAGTGCC133950.13905719533004518No Hit
GTTGGATCCGGCCCGGACTGGC130990.13598433756090045No Hit
ACCGATTGAATGGTTTAGTGAGGCCTC129270.13419875804639747No Hit
ACCCGTAGATCCGAACTTGTGG128190.1330775802117095No Hit
ACGGTTAGGCGAACAAGAATAGAGGCC124860.1296206152214217No Hit
GAGGTCCGGATTAGGGGAGGGTTACCCCT122480.1271498714746094No Hit
TCACTTTCTGGAGTTAAGGCT121110.1257276366287553No Hit
CTCGGGCTGAGACTTGAAGCGGT118310.12282087927956435No Hit
CTTTCTGGAGTTAAGGCT117320.1217931329311004No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA116910.12136750060496887No Hit
CGTGGTGGACTGCTTGCAGTGCTC113140.11745375945980821No Hit
CCGATTGAATGGTTTAGTGAGGCCTC112570.11686202671372292No Hit
GCCGAGCTGTTCTTCCTCGCCGAGAC111630.11588618674649453No Hit
CTATTTTGTTGGTTTCTGGAACC111240.11548131697285721No Hit
GAGACCGAGTGTGCTCTTAACTGAGTGT110800.11502454081798436No Hit
CTCGGGCTGAGACTTGAAGCGGTTG110650.11486882167427771No Hit
CTAGTACTGTGAAACTGCGAATGGCT106750.1108201239379046No Hit
CCGAGCTGTTCTTCCTCGCCGAGAC105580.10960551461699267No Hit
GAGACCGAGTGTGCTCTTAACTGA101670.10554643560437248No Hit
GACTGGCCGTGGTCGAGGC101330.10519347221197071No Hit
AGTTAAGCCCTGTCGGGCGGGGTTAGTACTT99220.1030030229238304No Hit
CCTGACTGGCGCCGACACT98060.10179879487916559No Hit
CGAATCTTTGAACGCAAATGGCGCTCTT98050.10178841360291846No Hit
CACTTTCTGGAGTTAAGGCT97920.10165345701170603No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)