FastQCFastQC Report
Wed 30 Apr 2025
1A5-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1A5-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11304593
Total Bases267 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGGCTGAGACTTGAAGCG10231659.050878700365418No Hit
TGAAAATCTTTGCTCTGAAGTGGAA3939143.4845482716626774No Hit
CTCGGGCTGAGACTTGAAGC2779442.4586820595841No Hit
GTTGGATCCGGCCCGGACTGGCC2000031.76921893605546No Hit
CTCGGGCTGAGACTTGAAGCGGTT1736661.5362428351025108No Hit
CGACTGCTGCCGGTCGTTGGGGCG1609941.4241468047544923No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT1593001.4091617451419967No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC1521601.3460015765273459No Hit
TCTCGGGCTGAGACTTGAAGCG1486311.3147841766616455No Hit
CAGCTGTGACCCTCGGGTC1422421.258267325502121No Hit
CCGAGCTGTTCTTCCTCGCCGAGACC1304901.154309580185682No Hit
TGAAAATCTTTGCTCTGAGGTGGAA1246161.1023483994514442No Hit
CGGACTGGCCGTGGTCGAGGC1083150.95815037303864No Hit
CAGCTGTGACCCTCGGGT1043330.9229257523910857No Hit
GAGTGTGCTCTTAACTGA995440.8805624404169172No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA934060.8262659257170957No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC884170.7821334213447578No Hit
CGAGTGTGCTCTTAACTG782470.6921699878978395No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC781910.6916746140263519No Hit
GGATCCGGCCCGGACTGGCC736610.6516024062078131No Hit
CTCGGGCTGAGACTTGAAGCA694420.6142812925684278No Hit
GATCCGGCCCGGACTGGCC650660.5755713628964794No Hit
GAGGTGTAGAATAAGTGGGAGCAGCTCGC649160.5742444685978523No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCA629540.5568886911718095No Hit
CGTGGTGGACTGCTTGCAGTGCT627050.5546860466360886No Hit
CTTTCCGAGGTCGCGGTC554420.4904378246965636No Hit
GAGTGTGCTCTTAACTG546800.4836972016595379No Hit
GAGTGTGCTCTTAACT538130.47602775261347313No Hit
CTCGGGCTGAGACTTGAAGCGG444900.3935568489728025No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCC444770.39344185146692146No Hit
CGACCAAGATAGTCGAACTCGACCCTGTT422480.3737242021893225No Hit
GCCGAGCTGTTCTTCCTCGCCGAGACC413180.3654974575378344No Hit
TCTCGGGCTGAGACTTGAAGCGGTT381600.3375619095707382No Hit
CTCGGGCTGAGACTTGAAGCGGT374670.3314316579110809No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA367170.32479718641794536No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTC355400.3143854891547179No Hit
TCTCGGGCTGAGACTTGAAGC353130.3123774557827955No Hit
TTGGATCCGGCCCGGACTGGCC340550.30124923559830946No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAT324400.28696300698309085No Hit
GTTCTATTTTGTTGGTTTCTGGAACC286320.2532775837219438No Hit
GAGACCGAGTGTGCTCTTAACTG282620.2500045777853303No Hit
GACCAAGATAGTCGAACTCGACCCTGTC277700.24565236448583333No Hit
GAGGTGTAGAATAAGTGGGAGCAGCT262660.23234803765159878No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG251860.22279439870148354No Hit
GAATCGGCCGTGGATTGGC249570.22076867340557949No Hit
CTTTCCGAGGTCGCGGTCGGA249370.22059175416576254No Hit
AGTGTGCTCTTAACT234810.2077120335070887No Hit
CCGAGCTGTTCTTCCTCGCCGAGAC233490.2065443665242968No Hit
CACTTTCTGGAGTTAAGGCT219810.1944430905208175No Hit
AGTGTGCTCTTAACTG215640.19075432437063414No Hit
TGGATCCGGCCCGGACTGGCC213880.189197435060245No Hit
GTTGGATCCGGCCCGGACTGGC213450.18881705869463855No Hit
GGAAGGAATCGGCCGTGGATTGGCC202810.17940495513637686No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA200300.17718461867667418No Hit
CGACTGCTGCCGGTCGTTGGGGCGGT199010.17604348957985483No Hit
CTTTCTGGAGTTAAGGCT195040.1725316426694884No Hit
GAGACCGAGTGTGCTCTTAACT194430.1719920389880467No Hit
CGACCAAGATAGTCGAACTCGACCCTGT190580.16858634362157046No Hit
CGAGTGTGCTCTTAACTGA185430.164030673196284No Hit
GTTGGATCCGGCCCGGACT183120.16198725597639826No Hit
GAGACTTGAAGCGGTTGGAT166440.14723219137566473No Hit
GGACTGGCCGTGGTCGAGGC163270.1444280214245661No Hit
GTTGGATCCGGCCCGGACTGGCT162880.14408302890692304No Hit
AGTGTGCTCTTAACTGA162290.14356111714946307No Hit
CGGGCTGAGACTTGAAGC157190.13904967653413086No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA147520.13049563128898137No Hit
CGACTGCTGCCGGTCGTTGGGGC146600.12968180278582342No Hit
GAGTGTGCTCTTAACTGAG144900.12817798924737936No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTCA141990.12560381430804277No Hit
CCGAGCTGTTCTTCCTCGCCGAGACCG140970.12470152618497632No Hit
GCACTGTGGTTCAGTGGTAGAATTCT140940.12467498829900378No Hit
CTCGGGCTGAGACTTGAAGCGGTTG139360.12327732630444989No Hit
TCGGGCTGAGACTTGAAGCG138090.12215388913161225No Hit
CGACTGCTGCCGGTCGTTGGGGCA135030.11944702476241294No Hit
GACTTGAAGCGGTTGGAT127810.11306024020502109No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTT127700.11296293462312178No Hit
GAGACCGAGTGTGCTCTTAACTGA125730.11122028011092483No Hit
CTGTGCCGACGACTGGAGACAC124270.10992876966026109No Hit
GAGACTTGAAGCGGTTGGA122010.10792958225032959No Hit
GACTGCTGCCGGTCGTTGGGGCG119240.10547925077886483No Hit
GACCAAGATAGTCGAACTCGACCCTGTT117620.10404620493634756No Hit
CTCGGGCTGAGACTTGAAGT117530.10396659127842993No Hit
GGAATCGGCCGTGGATTGGC114650.10141895422506586No Hit
CCCGGCTAGCTCAGTCGGTAGAGCATGA113270.10019821147032892No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)