FastQCFastQC Report
Wed 30 Apr 2025
1A4-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1A4-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7301529
Total Bases177.5 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGTGGACTGCTTGCAGTGCT4498806.1614491978323995No Hit
CGTAGTTGGATTTCGGGATGGCCCG1948962.6692491394610633No Hit
CAGCTGTGACCCTCGGGTC1665172.2805771229560277No Hit
CTATTTTGTTGGTTTCTGGAACCGAAGTAAT664560.9101655283434471No Hit
CGGACTGGCCGTGGTCGAGGC615390.8428234688926114No Hit
CTCGGGCTGAGACTTGAAGCG601180.8233617917562198No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA556820.7626073936020797No Hit
CTCGGGCTGAGACTTGAAGCGGTT503140.6890885457004965No Hit
CGTGGTGGACTGCTTGCAGTGCC427020.5848364089220217No Hit
CTCGGGCTGAGACTTGAAGC415220.5686754103147437No Hit
CGTGGTGGACTGCTTGCAGT413160.5658540834392358No Hit
CGGTTAGGCGAACAAGAATAGAGGCC380440.5210415517078684No Hit
CTAGTACTGTGAAACTGCGAATGGCT373980.5121940897584601No Hit
CGTGGTGGACTGCTTGCAGTGCTC356610.48840455197808563No Hit
CCGCGACTGCTGCCGGTCGTTGGGGCGGT352050.48215928471967995No Hit
CTGACTGGCGCCGACACT323580.4431674516392388No Hit
CAGCTGTGACCCTCGGGT302850.41477613798424956No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAT291200.39882057579994545No Hit
ACGGTTAGGCGAACAAGAATAGAGGCC283440.38819266485143045No Hit
GAGACCGAGTGTGCTCTTAACT280040.3835361059306893No Hit
ACGATCTTCTGAGATTAAGCCTTTGTT277990.38072847481671307No Hit
GAGACTTGAAGCGGTTGGA268480.3677038056001695No Hit
GAGACTTGAAGCGGTTGGAT251990.3451194948345751No Hit
CGTCGTCGTGCTGGGGATA249980.3423666467667251No Hit
GAGACCGAGTGTGCTCTTAACTG220730.3023065442868199No Hit
CGGTTAGGCGAACAAGAATAGAGGC220460.30193675872546694No Hit
CGCGTAGAACAAAAGGGTAAAAGCT217530.29792390059671064No Hit
ACGATCTTCTGAGATTAAGCCTTTGT217520.29791020483517905No Hit
CGTAGTTGGATTTCGGGATGGCCCA214710.2940616958448018No Hit
GATAAACGTCGTCGTGCTGGGGATA214400.2936371272373225No Hit
GAATCGGCCGTGGATTGGC197250.270148896210643No Hit
ACTGCTGCCGGTCGTTGGGGC192740.2639721077598952No Hit
CGTGGTGGACTGCTTGCAGTGCA166330.22780160155496196No Hit
GAGTGTGCTTGGGATCTGC157570.21580411445328782No Hit
GAGTGTGCTTGGGATCTGCGA155280.21266778506255335No Hit
CTATTTTGTTGGTTTCTGGAACC151460.20743600415748537No Hit
CTGACTGGCGCCGACACTCTGTCTCGTGCA150440.20603903648126304No Hit
CTGACTGGCGCCGACACTCT147960.2026424876214283No Hit
AGTGTGCTTGGGATCTGCGA143970.19717787877032328No Hit
GAAAGACGAACTACTGCGAAAGCATT142920.1957398238095062No Hit
GATCGAATCGCACGGCCTGTGCGCT135790.18597474583748144No Hit
GGAAGGAATCGGCCGTGGATTGGCC135580.185687134845318No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC132800.18187971313953558No Hit
GAGTGTGCTTGGGATCTGCGACGTT131610.18024991751727618No Hit
GCCGGGAAGTTGTTCAAACTTGATCA131570.1801951344711498No Hit
GCTCGTAGTTGGATTTCGGGATGGCCCG131400.17996230652511275No Hit
GGACTGGCCGTGGTCGAGGC130200.17831881514132178No Hit
GCCGGTCTGCCGCGAGGTACGTT130050.1781133787183479No Hit
GAGGTGTAGAATAAGTGGGAGCAGCT129750.17770250587240016No Hit
GACTGCTGCCGGTCGTTGGGGCGGT125070.1712928894756153No Hit
GAACTGGGCGTCGGAGGTGCCGTGGAAGGTA122950.1683893880309179No Hit
AGTGTGCTTGGGATCTGCGACGTT120680.16528045016324663No Hit
CGACTGCTGCCGGTCGTTGGGGCGGT120140.16454087904054068No Hit
AGTACTGTGAAACTGCGAATGGCT116410.15943235998925703No Hit
AGTCTTGTAATTGGAATGAGTACA115820.15862431005889316No Hit
GACCGTGGTGGACTGCTTGCAGTGCT113340.15522776119905846No Hit
AGTGTGCTTGGGATCTGC108560.1486811871869577No Hit
CTATTTTGTTGGTTTCTGGAACCGAAGT108390.14844835924092062No Hit
GAAAGACGAACTACTGCGAAAGC108020.14794161606425174No Hit
GAGGTCCGGATTAGGGGAGGGTTACCCCT107290.14694182547244555No Hit
CGTGGTGGACTGCTTGCA107200.14681856361866125No Hit
GAGGTGTAGAATAAGTGGGAGCAGCTCGC104300.14284679277449971No Hit
CTCTTGGGCGCGCGTCAGCGTGGGTCG103870.14225787502864126No Hit
GCCGGGAAGTTGTTCAAACTTGATC102890.1409156903985453No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC102470.14034046841421846No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCC101790.13940915663007022No Hit
GTTCTATTTTGTTGGTTTCTGGAACC101130.13850523636898518No Hit
CTTAACTGAGTGTGCTTGGGAT99310.13601260777023552No Hit
ACGGTTAGGCGAACAAGAATAGAGGC99050.13565651797041414No Hit
CTGCGACGTTTACTTTGAAAAAATTAGAGT97460.13347889188689108No Hit
CGTGGTGGACTGCTTGCAGTGCG97360.13334193427157517No Hit
CGACTGCTGCCGGTCGTTGGGGC96620.1323284479182374No Hit
CTCCTGACTGGCGCCGACA96220.13178061745697373No Hit
GATCGAATCGCACGGCCTGTGCGCTGGC93510.12806906608191243No Hit
CTCGTAGTTGGATTTCGGGATGGCCCG93350.12784993389740698No Hit
CGCACGGCCTGTGCGCTGGCGATGTTTCATT93040.12742536528992762No Hit
CATCGAATCTTTGAACGCAAATGGCGCTCTT93010.12738427800533286No Hit
GGAAGGAATCGGCCGTGGAT92500.1266857941672217No Hit
CTGATAAACGTCGTCGTGCTGGGGATA92000.12600100609064213No Hit
GAAAGACGAACTACTGCGAAAGCAT89270.12226206319251763No Hit
CATAGTGAGGATTGACAGATTGAGAGCT87980.12049530995494231No Hit
CGTAGTTGGATTTCGGGATGGCC87630.1200159583013366No Hit
CGAATCTTTGAACGCAAATGGCGCTCTT86780.11885181857115135No Hit
CTCGCGTAGAACAAAAGGGTAAAAGCT85420.11698919500285489No Hit
GACTGCTGCCGGTCGTTGGGGC85170.11664680096456509No Hit
GGAAGGAATCGGCCGTGGATT83880.11488004772698979No Hit
CTTCTCTTGGGCGCGCGTCAGCGTGGGTCG81080.11104523449814414No Hit
CTTAACTGAGTGTGCTTGGGATCTGC80980.11090827688282824No Hit
AGCTGTGACCCTCGGGTC79840.10934696006822682No Hit
GAAGTAATGATTGAGAGAGACA79570.10897717450687383No Hit
TGGGGGTAGTCGTTCTATGT79370.108703259276242No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT77810.10656672047731372No Hit
CGCGTAGAACAAAAGGGTAAAAGCTCG77710.10642976286199782No Hit
CTGGGCGTCGGAGGTGCCGTGGAAGGTAGGT77670.10637497981587143No Hit
GCGACGTTTACTTTGAAAAAATTAGAGT77610.1062928052466819No Hit
AAAGACGAACTACTGCGAAAGCATT76830.10522453584721775No Hit
GTTGGATCCGGCCCGGACTGGCC75500.10340299956351608No Hit
GAGGTGCCGTGGAAGGTAGGT75470.1033619122789213No Hit
GGCGATGCGGGATGAACCGAACGCGGAGT75340.10318386737901061No Hit
CCGATTGAATGGTTTAGTGAGGCCTC75200.10299212671756833No Hit
AGACTTGAAGCGGTTGGAT75200.10299212671756833No Hit
AGTGTGCTTGGGATCTGCGAC74910.10259494963315219No Hit
GAGTGTGCTTGGGATCTGCGAC74790.10243060049477308No Hit
CGAGTGTGCTCTTAACTG74760.1023895132101783No Hit
AAAGACGAACTACTGCGAAAGC73260.10033514898043958No Hit
TGAAAATCTTTGCTCTGAAGTGGAA73040.10003384222674455No Hit
CGTGGTGGACTGCTTGCAGTGC73030.10002014646521297No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)