FastQCFastQC Report
Wed 30 Apr 2025
1A3-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1A3-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17059800
Total Bases458.4 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA269599215.803186438293531No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC6192093.6296380965779202No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT1688500.9897536899611953No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1536790.900825332067199No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC1345070.7884441787125289No Hit
ACCCGTAGATCCGAACTTGTGG1211470.7101314200635412No Hit
CGGACTGGCCGTGGTCGAGGC1041300.6103823022544227No Hit
TCAAGTCTAGGCTGGTTAGTTT754380.4421974466289171No Hit
TGAAAATCTTTGCTCTGAAGTGGAT736560.43175183765343084No Hit
CAGCTGTGACCCTCGGGTC705510.4135511553476594No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA675710.3960831897208642No Hit
TAGGTGCTGCCCTGCAAATTCCCATATATC625480.3666397026928803No Hit
TGAAAATCTTTGCTCTGAAGTGGA623880.36570182534379064No Hit
ACCCGTAGATCCGAACTTGT544250.319024842026284No Hit
TTGTGTAACTCCCTAAGGAAGG524800.3076237705014127No Hit
CGTGGTGGACTGCTTGCAGTGCT480880.2818790372689011No Hit
TGAAAATCTTTGCTCTGAAGTGG431550.2529631062497802No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT419670.24599936693278937No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA414340.2428750630136344No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA406140.23806844159954982No Hit
CACCCCTCATCCGCTACTTTGACCTCATT375460.22008464343075532No Hit
TTACCGTGACCGATCCCGT360150.21111032954665354No Hit
TCGTCTGATTGGATACCCACGTACTTGATC345960.2027925298069145No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC337810.19801521705998898No Hit
TCAGTCCCACCATCTCACCAAT336650.19733525598189897No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT333980.19577017315560558No Hit
TGTCCACCCCCCACCGGCTGTTTTTTGGTC297210.17421657932683854No Hit
TTCCTGCACGGCTTCAGGCAATTCTGGC292230.17129743607779693No Hit
TGTGCGTGTTTTCACTGCGGCGACTGTCGC281570.16504882823948697No Hit
CGATCAAGATAGTCGAACTCGACCCTGTC278610.1633137551436711No Hit
GTTGGATCCGGCCCGGACTGGCC278360.16316721180787583No Hit
CGGACTGGCCGTGGTCGAGGT272850.15993739668694826No Hit
TTCAATGTGAAAAGTCTACTTAGGATGAGT257270.15080481600018758No Hit
TCTTTTCAGATTCTGTAATGACCATAAAC254610.149245594907326No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT241610.1416253414459724No Hit
TCAGGGATTGTGGTGAGTTAGTT233540.13689492256650138No Hit
CTCGGGCTGAGACTTGAAGCG227680.13345994677546044No Hit
TTCCAGCACCGCTTCAGGCAATTCAGGC217830.12768613934512713No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAT217650.12758062814335455No Hit
TGAAAACCTTTGCTCTGAAGTGGAA215530.12633794065581072No Hit
TCGAAGACTCTAAACGGCGGAAGCCTATT210810.1235712024759962No Hit
TGAAAATCTTTGCTCTGAAGTGGAG207730.12176578857899858No Hit
ACCCGTAGATCCGAACTTGTGA205910.12069895309440909No Hit
TGCACCAGTTTGACCGTGATTGCTCGTTGG204700.11998968334916002No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG200410.11747499970691333No Hit
TTAAATGAAGTTTCCAATGTTTGACTGTC189800.11125570053576245No Hit
TGCTTCAAGGGCAGTTAGACCAAATCCTTC187630.10998370438105957No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAA186200.10914547650031069No Hit
TGATCATGGATTCTAAACTGGTAATGGCC182610.10704111419829072No Hit
TGATCTGCGCATAGGAATTGCCCTGGACTGC180510.10581015017761052No Hit
GTTTGACAACTTTTGACGGT179390.10515363603324775No Hit
TGAAAATCTTTGCTCTGAAGTGGGA175650.10296134772975064No Hit
TGATAGAGCTTCATCTTCTTCGCTGATGA172860.10132592410227552No Hit
TAGGTGCTGCCCTGCAAAGTCCCATATATC171970.10080422982684441No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA170760.10009496008159532No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)