FastQCFastQC Report
Wed 30 Apr 2025
2H2-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2H2-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13183253
Total Bases351.3 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA315737523.94989309542948No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC4155553.152143101554677No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGC3146682.386876744305825No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC980180.7435038984687619No Hit
TGAAAATCTTTGCTCTGAAGTGGA810620.6148861741483683No Hit
TCAGGTCTAGGCTGGTTAGTTT760570.5769213410377545No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA712020.5400943151132729No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT659040.4999069652990806No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTT651530.4942103439871783No Hit
TAAAATGTAAAATATGACGCTCCTAAAAGG471100.3573473102579462No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT446960.33903619994245726No Hit
TGAAAATCTTTGCTCTGAAGTGGGA407160.3088463826037473No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGT368490.27951371334525704No Hit
TCCCGTAGATCCGAACTTGT314570.23861333769442183No Hit
GAGGTCCGGACGGAGGAGGGTTATCCAC313210.2375817258456619No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC312710.23720245678361782No Hit
AGCACTGATGACTGTTCAGTTTTTCTGAATT311100.23598121040383585No Hit
TATTAATGCTGTAGGTGAAAGACA288920.21915683481155981No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC255090.19349549007365632No Hit
TGAAAATCTTTGCTCTGAAGTGG227460.17253708170509965No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAA192310.14587446664339976No Hit
CTCGGGCTGAGACTTGAAGCGGGT190890.14479734250719456No Hit
TGAAAATCTTTGCTCTGAAGTGGTA189660.14386434061456607No Hit
TTGTTTATGCCGTGAGGATTGGAGGAAAA186280.14130048175514798No Hit
TGAAAATCTTTGCTCTGAAGTGGAG169540.1286025535579117No Hit
TTGTAGTTGATTAAGTGGTAATAGCCAGGC168340.12769230780900587No Hit
TATCAGCATCTGTTCAGCATCTGACGTGT162310.12311832292075409No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT158660.12034965876783218No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT157990.1198414382246931No Hit
TTGGAAGGGATTTGAATT155420.11789199524578646No Hit
TGATTAGATCTAATGGTTTTTGAGCAAATT154690.11733826241520208No Hit
TGAAAATCCTTGCTCTGAAGTGGAA146760.11132305509118272No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGCG145070.1100411256614737No Hit
TTCGTGTGGCTTCTCTGACTTAGACATC142840.10834958564475702No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGC141900.10763655980811412No Hit
CCCAGGAGCATGTCTGTCTGAGTGTCGGA141170.10708282697752974No Hit
TCCCGTAGATCCGAACTTGTGG139680.10595260517263835No Hit
TGAAAATCTTTGCTCTGAGGTGGAA138500.10505753018621429No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA133150.10099935122234248No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)