FastQCFastQC Report
Wed 30 Apr 2025
2H1-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2H1-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5450
Total Bases134.1 kbp
Sequences flagged as poor quality0
Sequence length15-31
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA3386.201834862385321No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC1202.2018348623853212No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC971.7798165137614679No Hit
TGAGGTAGTAGGTTGTATGGTT921.6880733944954127No Hit
TGAGGTAGTAGATTGTATAGTT861.5779816513761469No Hit
TGAGGTAGTAGTTTGTGCTGTT761.3944954128440368No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT490.8990825688073394No Hit
TCAGTGCACTACAGAACTTTGT490.8990825688073394No Hit
TTCAAGTAATCCAGGATAGGCT440.8073394495412843No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC430.7889908256880734No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC390.7155963302752293No Hit
TTCACAGTGGCTAAGTTCTGC320.5871559633027523No Hit
TGAGGTAGTAGGTTGTATAGTT290.5321100917431192No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT250.45871559633027525No Hit
TACCCTGTAGATCCGAATTTGT240.44036697247706424No Hit
TTTGGCACTAGCACATTTTTGCT240.44036697247706424No Hit
TGAGGTAGTAGATTGTATAGT230.4220183486238532No Hit
AGCTGGTGTTGTGAATCAGGCCGT230.4220183486238532No Hit
TGAAAATCTTTCTTCTGAGGCGGAA220.40366972477064217No Hit
TGAGATGAAGCACTGTAGCTCT190.3486238532110092No Hit
TGTAAACATCCTCGACTGGAAG190.3486238532110092No Hit
TGAGGTAGTAGTTTGTGCTGT170.3119266055045871No Hit
TGAGATGAAGCACTGTAGCTC160.29357798165137616No Hit
TCCCGTAGATCCGAACTTGTGG160.29357798165137616No Hit
TCTTTGGTTATCTAGCTGTATG160.29357798165137616No Hit
TGTAAACATCCTCGACTGGA150.27522935779816515No Hit
TAGCTTATCAGACTGATGTTGAC140.25688073394495414No Hit
TGAGGTAGTAGTTTGTACAGTT140.25688073394495414No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC140.25688073394495414No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGC130.23853211009174313No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGC130.23853211009174313No Hit
TGAGGTAGTAGTTTGTGCTGTC130.23853211009174313No Hit
TGGAATGTAAAGAAGTATGTAT120.22018348623853212No Hit
TCGTACCGTGAGTAATAATGCG120.22018348623853212No Hit
AACCCGTAGATCCGATCTTGT110.20183486238532108No Hit
TCCCGTAGATCCGAACTTGT110.20183486238532108No Hit
TGAGGTAGTAGGTTGTGTGGTT110.20183486238532108No Hit
TGAAAATCTTTGCTCTGAAGTGGA110.20183486238532108No Hit
TGAGGTAGTAGGTTGTATGGTTT110.20183486238532108No Hit
TGAGGTAGTAGGTTGTATAGT110.20183486238532108No Hit
TGGCTCAGTTCAGCAGGAACAG100.1834862385321101No Hit
TTCACAGTGGCTAAGTTCTGT100.1834862385321101No Hit
TCTTTGGTTATCTAGCTGTATGA100.1834862385321101No Hit
TCAGTGCACTACAGAACTTTGC100.1834862385321101No Hit
TAGTAACAATGACCTGATCTGGAGACTCC100.1834862385321101No Hit
TGAGGTAGTAGATTGTATAGTC100.1834862385321101No Hit
CTAGCACCATCTGAAATCGGTT100.1834862385321101No Hit
GTTCGCGCTAGTCCCCGCA100.1834862385321101No Hit
TTTGGCAATGGTAGAACTCACACC90.1651376146788991No Hit
TACCCTGTAGAACCGAATTTGT90.1651376146788991No Hit
TGTAAACATCCTCGACTGGAAGCT90.1651376146788991No Hit
TTCACAGTGGCTAAGTTCTG90.1651376146788991No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC90.1651376146788991No Hit
ACTGGTGGTTCAGTGGTAGAATTCTC90.1651376146788991No Hit
TGAGGTAGTAGGTTGTATGGT90.1651376146788991No Hit
GGCTGTTTGGTCTAGTGGTATGATTCC90.1651376146788991No Hit
TCGTACCGTGAGTAATAATGC90.1651376146788991No Hit
AAGCTGCCAGTTGAAGAACTGT90.1651376146788991No Hit
TTCAAGTAATCCAGGATAGGCA90.1651376146788991No Hit
TTCAAGTAATCCAGGATAGGCC90.1651376146788991No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT90.1651376146788991No Hit
TGAGATGAAGCACTGTAGCT90.1651376146788991No Hit
TGTAAACATCCTTGACTGGAAGCT90.1651376146788991No Hit
ACAGTAGTCTGCACATTGGTT80.14678899082568808No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA80.14678899082568808No Hit
ACCCTGTAGATCCGAATTTGT80.14678899082568808No Hit
TCGTACCGTGAGTAATAATG80.14678899082568808No Hit
TACCCTGTAGAACCGAATTTGTG70.12844036697247707No Hit
TGAAAATCTTTGCTCTGAAGTGGGAA70.12844036697247707No Hit
TATCAACTGTGCTGCACTCTGA70.12844036697247707No Hit
TGAGGTAGGAGGTTGTATAGTT70.12844036697247707No Hit
TTCAAGTAATCCAGGATAGGC70.12844036697247707No Hit
TCGGATCCGTCTGAGCTTGGCTT70.12844036697247707No Hit
CGGAGGAGGTGATCGCATTCTGAGCGGT70.12844036697247707No Hit
TTCAAGTAATCCAGGATAGGCTT60.11009174311926606No Hit
TGAGGTAGTAGGTTGTGTGGTTT60.11009174311926606No Hit
TTGTGCTTGATCTAACCATGT60.11009174311926606No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTC60.11009174311926606No Hit
TGTAAACATCCTCGACTGGAAGCA60.11009174311926606No Hit
TAGCTTATCAGACTGATGTTGA60.11009174311926606No Hit
TGTAAACATCCTACACTCAGCT60.11009174311926606No Hit
TGGCTCAGTTCAGCAGGAAC60.11009174311926606No Hit
ACCCTGTAGATCCGAATTTGTGA60.11009174311926606No Hit
TATGGCACTGGTAGAATTCACT60.11009174311926606No Hit
ATGGCACTGGTAGAATTCACT60.11009174311926606No Hit
TCCAAGACCTGAGTTACCACTGACAAGC60.11009174311926606No Hit
TGAGGTAGTAGATTGTATAGTTT60.11009174311926606No Hit
TTCAAGTAATCCAGGATAGG60.11009174311926606No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC60.11009174311926606No Hit
TGATTGGTTCTCATGTGACCTCATAGGAAGC60.11009174311926606No Hit
TAGCACCATCTGAAATCGGTTA60.11009174311926606No Hit
TGAGGTAGTAGTTTGTGCTGTTT60.11009174311926606No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)