FastQCFastQC Report
Wed 30 Apr 2025
2F2-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2F2-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63669
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA32605.12023119571534No Hit
GATCGGCTCATTAATTCATGGA5900.9266676090405064No Hit
CTCGGGCTGAGACTTGAAGCGG4310.6769385415194207No Hit
CTCGGGCTGAGACTTGAAGCGGGT4170.6549498185930359No Hit
GTGTCTTCACGAGCTTGCTCTGGA3340.5245881041008968No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC3220.5057406273068527No Hit
GTGGAGCCGGCCTGGGCTG2990.4696162967849346No Hit
GTATTGTAAGTGCTGAGAGT2650.41621511253514265No Hit
CATCTTGCTGAAGAACGTATCTGCA2420.38009078201322466No Hit
CGGGCTGAGACTTGAAGCGGGT2380.37380828974854324No Hit
GTGTGCTCAGGATCTGTGAC2020.31726585936641066No Hit
GATAAATTGTAAACTCCA1960.30784212096938857No Hit
CTCGGGCTGAGACTTGAAGCG1870.2937065133738554No Hit
TGATCGGCTCATTAATTCATGGA1750.2748590365798112No Hit
TGAAAATCTTTGCTCTGAAGTGGA1640.25758218285193735No Hit
TCTCGGGCTGAGACTTGAAGCGGGT1520.23873470605789318No Hit
TGAGGTAGTAGTTTGTGCTGTT1490.2340228368593821No Hit
AGTGTGCTCAGGATCTGTGAC1430.22459909846236004No Hit
GATCGGCTCATTAATTCATG1360.21360473699916757No Hit
GTGTGCTCAGGATCTGTGA1260.19789850633746406No Hit
TCTCGGGCTGAGACTTGAAGCGG1250.19632788327129375No Hit
CGACCGACTGTATAGCAAATA1170.18376289874193094No Hit
TGAGGTAGTAGGTTGTATGGTT1160.18219227567576057No Hit
GTGATAGATCTTTTGGAGA1150.18062165260959023No Hit
GATGAATTGTAAACTCCA1150.18062165260959023No Hit
CATAGTAACTGATCGAATC1120.17590978341107918No Hit
ATGATCGGCTCATTAATTCATGGA1090.1711979142125681No Hit
CGGGCTGAGACTTGAAGCGG1080.1696272911463978No Hit
GTGATAGATCTTTTGGAGACGA1070.16805666808022743No Hit
GTGCTCAGCTTCTGGACGTTGC1060.1664860450140571No Hit
CTCGGGCTGAGACTTGAAGCGGGTG1060.1664860450140571No Hit
TAAAAAACTCGTCGACGTG990.15549168355086462No Hit
CGTTAAAGGCGTAGAAGCA970.15235043741852392No Hit
GAGGTCCGGACGGAGGAGGGTTATCCAC970.15235043741852392No Hit
CGACCGACTGTATAGCAAATAC960.15077981435235357No Hit
GTGTGCTCAGGATCTGTGACGTTTAC940.1476385682200129No Hit
ATGCACATTGCTAGAACCTGGCAGA920.1444973220876722No Hit
GCTGAGGGTACTGTCGGCT910.14292669902150182No Hit
CGAGTTAGATAGAATAAGGTC860.13507358369065006No Hit
CGACCGACTGTATAGCAAATACA830.13036171449213904No Hit
TGAGGTAGTAGATTGTATAGTT820.12879109142596867No Hit
AATGGTGTTTATCGACCGACTGTATAGC810.12722046835979833No Hit
GAGTGTGCTCAGGATCTGTGAC800.125649845293628No Hit
CATAGTAACTGATCGAAT790.12407922222745763No Hit
AGTGTGCTCAGGATCTGTGA780.12250859916128728No Hit
TATCGACCGACTGTATAGCAAATA760.11936735302894659No Hit
CAGCTATGGCCGCGAGGTC760.11936735302894659No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAA740.1162261068966059No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGC720.11308486076426517No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC710.11151423769809483No Hit
CGAGTTAGATAGAATAAGG710.11151423769809483No Hit
TTCAAGTAATCCAGGATAGGCT690.10837299156575414No Hit
ATTGTAAGTGCTGAGAGT680.10680236849958379No Hit
CGACCGACTGTATAGCAAATACAC660.10366112236724308No Hit
GCTTGCGAGCTGTTGCCA650.10209049930107275No Hit
GGGAAGTTGGTCAAACTTGATC640.10051987623490238No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)