FastQCFastQC Report
Wed 30 Apr 2025
2E3-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2E3-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95
Total Bases2.5 kbp
Sequences flagged as poor quality0
Sequence length18-31
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC55.263157894736842No Hit
TGAAAATCTTTGCTCTGAAGTGGAA44.2105263157894735No Hit
GTTGGATCCGGCCCGGACTGGCC22.1052631578947367No Hit
GTTCGCGCTAGTCCCCGCT22.1052631578947367No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA22.1052631578947367No Hit
TGATTGGTTCTCATGTGACCTCATAGGAAG22.1052631578947367No Hit
TGAAAATCTTTCTTCTGAGGCGGAA22.1052631578947367No Hit
AAACAAGTTTGACTGATGAGGA11.0526315789473684No Hit
TAGTTATCAGTCAACGCTTTCTCCGGGGT11.0526315789473684No Hit
CTGAGCCGAGTTGCGAATACGACCC11.0526315789473684No Hit
GCTCTGGTGGTTCAGTGGTAGAATTCTCGCC11.0526315789473684No Hit
CGTAGAACAAAAGGGTAAAAGCT11.0526315789473684No Hit
GCACTGTGGTTCAATGGTAGAATTCTCGCCT11.0526315789473684No Hit
CGGAGTGTAGCTCAGCCTGGTAGAGCACT11.0526315789473684No Hit
TTGTACCACTGTTACTGGACGAAGACGT11.0526315789473684No Hit
GAGGTCCGGACTTGGGGAGGGTTATCC11.0526315789473684No Hit
TGCCTAGTAGTTGTTACAGAGCCGTCTCTGC11.0526315789473684No Hit
CACAGTTCAGATTGCAGGC11.0526315789473684No Hit
ATTTGCTGTACGTGTAGTCCATCCTCGTGG11.0526315789473684No Hit
TCAAAGTGAGATCTAGCTGAGTGAACATT11.0526315789473684No Hit
TAGTACTTTTGCTGAGTTCCTGAGACGT11.0526315789473684No Hit
TGGCTTAGTTGGTCCTTACTGAAAATAGC11.0526315789473684No Hit
TGGGGGTAGTCGTTCTATGTAGATAGGGT11.0526315789473684No Hit
GACCGGGAAGGAATCGGCCGTGGATTGGC11.0526315789473684No Hit
TATCCAACCACTGTAGAGCCAAGCAGGCAGG11.0526315789473684No Hit
TCATAGGCTCTACACTTCT11.0526315789473684No Hit
CCACTGTGATGACTAGAATAAACCCAGCT11.0526315789473684No Hit
TACATGCATTGATATGGACTTCTGACTG11.0526315789473684No Hit
GCACTGGTGGTTCAATGGTAGAATTCTCGCT11.0526315789473684No Hit
TCGCATAAACGCTATCTTTTCCCTCTCGC11.0526315789473684No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT11.0526315789473684No Hit
AGTCACGTTTAATTTGTAGCTCC11.0526315789473684No Hit
TTACGACTGGGGATATGCCATCTC11.0526315789473684No Hit
TCCTCGTTGTATAGTGGTGAGTATCCCC11.0526315789473684No Hit
TCCTGATTGTAAGAATTGAAAGTTTGAGG11.0526315789473684No Hit
GCGGACGGTAAGGTAGTGGCTTACC11.0526315789473684No Hit
AAGCACGTGTTTTCAGTCCTGTGGATA11.0526315789473684No Hit
GAGGTCCGGACTTGGGGAGG11.0526315789473684No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGC11.0526315789473684No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC11.0526315789473684No Hit
GAAGGTAAAATGTTCTGTAAATACTGTTTG11.0526315789473684No Hit
TCGTTAAAATCAAGCCCCCTCCTTGTAGGT11.0526315789473684No Hit
CGCGACCTCAGATCAGACGT11.0526315789473684No Hit
GTGGCTGTGATGATACTA11.0526315789473684No Hit
TGTGTATTCTGTAAATAAAGATATGGCAGC11.0526315789473684No Hit
TAAACGATCATCATCCCTTTTGGT11.0526315789473684No Hit
TCCAAGTCCTCATCCTCTTCCAAAACCGCC11.0526315789473684No Hit
TCTAAGGCCTGAGTTACCACTGACAAGC11.0526315789473684No Hit
GGTGCCGTGGAAGGTAGGTCTG11.0526315789473684No Hit
TTTCACATTTCCAATATGTCCCAGGCAAGT11.0526315789473684No Hit
GTTCGCGCTAGTCCCCGCA11.0526315789473684No Hit
TACGAAAACTCGGAAGTTAGCACCCTGGGGC11.0526315789473684No Hit
TTATTCGATTACTAAAGGACGGG11.0526315789473684No Hit
TACTTCAAGACGCAGACTGACCTTGGCC11.0526315789473684No Hit
TCAGTCACAAGGTATTGTTCAGCAACTCC11.0526315789473684No Hit
TCGGGCTGAGACTTGAAGCGGTT11.0526315789473684No Hit
CACCCCTCCCCGCACATTGACCTCTT11.0526315789473684No Hit
CGCGGGGTAGAGCAGTCTGGTAGCTCGC11.0526315789473684No Hit
TTCTTTGATATTGGCTTCATCTGGTCTGT11.0526315789473684No Hit
GTGAAATTCTTGGATTTGTTAAAGACG11.0526315789473684No Hit
TTGACCTCAGATCAGGCAAGGCTAC11.0526315789473684No Hit
TCTGTCTGGATTTGAGTCAAGTGAAATCT11.0526315789473684No Hit
CGAGTGTGCTCTTAACTG11.0526315789473684No Hit
TAGAGCTGATATGTGACCAGCGTGTGCA11.0526315789473684No Hit
AAAAGGATTCTAATAATGGTAGTTTTG11.0526315789473684No Hit
ATTTTAAAGAGCTCTGATTGGTACC11.0526315789473684No Hit
CTGTTAACACCGTGAAAGAGCA11.0526315789473684No Hit
ATCGCCTGTGAAATTAACTC11.0526315789473684No Hit
GCATGCATGTGCGCAACTCC11.0526315789473684No Hit
ACAACTAGATAGAATAAGGTC11.0526315789473684No Hit
AGTTTTGATGAATTGCACTGGGTTAC11.0526315789473684No Hit
TGGGAAGTAACTTTAAGCCATTGTCAATGT11.0526315789473684No Hit
TCACTGAGACAACGGAGACACG11.0526315789473684No Hit
ATGGATGAAGAAATCTTATGTAAGCTG11.0526315789473684No Hit
CATCTTCCTGAAGAACGTATCTGCAC11.0526315789473684No Hit
ACGAGACCTCGCTCTCTACATTGG11.0526315789473684No Hit
TTGGTGCTGTTTCACTTTTACAATAAGT11.0526315789473684No Hit
AATCTGCCTCTTGGACGGGGACAACAG11.0526315789473684No Hit
ACGGCGAAGATACTTGGAGGGTAGCC11.0526315789473684No Hit
TGAAAATCTTTCTTCTGAGGCGGA11.0526315789473684No Hit
GTACCGAAGCTGTGGACTTGC11.0526315789473684No Hit
CTAGTTGGTGGGGTAAGGGCTT11.0526315789473684No Hit
TGAAAATCTTTCTTCTGAGGCGGAG11.0526315789473684No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)