FastQCFastQC Report
Wed 30 Apr 2025
2E1-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2E1-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11237418
Total Bases274.8 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA353883531.491531239649532No Hit
TGAAAATCTTTGCTCTGAAGTGGA1108100.9860806103323735No Hit
TTGGAAGGGATTTGAATT716900.6379579366007387No Hit
GATCGGCTCATTAATTCATGGA347620.3093415231150074No Hit
TATTAATGCTGTAGGTGAAAGACA347240.30900336714359117No Hit
GTGGAGCCGGCCTGGGCTG335180.298271364471803No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGC312770.2783290609995997No Hit
CTCGGGCTGAGACTTGAAGCGGGT299640.26664488230303435No Hit
CTCGGGCTGAGACTTGAAGCGG282780.25164143578177833No Hit
TTGTTTATGCCGTGAGGATTGGAGGAAAA275260.2449495070842786No Hit
GAGGTCCGGACGGAGGAGGGTTATCCAC265210.23600617152445516No Hit
TGAAAATCTTTGCTCTGAAGTGGTA263710.23467134532149644No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC258210.22977698257731446No Hit
TAAAAAACTCGTCGACGTG252260.22448217197224488No Hit
TAGTTCAGTAGAGCGTTT252090.2243308916692429No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA239250.21290477937191624No Hit
CATCACGAATTTCCCTGCTCT216400.19257092688017835No Hit
TCTCGGGCTGAGACTTGAAGCGGGT203210.18083335513549464No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAA199630.1776475699310998No Hit
CTGGCAACCTACCGCTAGTGGATGTGT194840.17338502492298496No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT187480.16683547768713416No Hit
CACTAACTTGACCTCATT186560.16601678428265285No Hit
TAGTGATAGCGTCTTGGCTCAAAAAA181850.16182543000536245No Hit
TGAAAATCTTTGCTCTGAAGTGGGA177500.15795443401678214No Hit
TCAGGTCTAGGCTGGTTAGTTT165120.14693766842169617No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTT164340.1462435587961576No Hit
TGAAAATCTTTTCTCTGAAGTGGAA159400.1418475311677469No Hit
TAAAAAACTCGTCGACGTGA151280.1346216719890637No Hit
TGAAAATCCTTGCTCTGAAGTGGAA151130.1344881893687678No Hit
GAGGTCCGGACGGAGGAGGGTTAT139680.12429901601951623No Hit
TGAAAATCTTTGCTCTGAGGTGGAA137090.12199421610907416No Hit
CATCTTACATGTATGTTTGAACTCATATGT135160.12027673972793394No Hit
CTCGGGCTGAGACTTGAAGCG129760.11547136539728255No Hit
TTCTACCGTGTATGACTTGCGAGT129240.11500862564692352No Hit
TCATACAATGGACAAGGATCAGG124360.11066599106663115No Hit
CATCTTGCTGAAGAACGTATCTGCA122480.1089930088922562No Hit
TGAAAATCTTTGCCCTGAAGTGGAA122140.10869044828625224No Hit
CGACAGCTGGAGACATCC120170.10693737653969977No Hit
TATTAATGCTGTAGGTGAAAGACCA119140.1060207958803348No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)