FastQCFastQC Report
Wed 30 Apr 2025
2D3-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2D3-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58131
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT8011.3779222789905559No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC7251.247183086477095No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC4850.8343224785398496No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCT4440.7637921246839036No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT4050.6967022758941013No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCC4040.6949820233610294No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGC3070.5281175276530594No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGT2990.5143555073884847No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGC2770.4765099516609039No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGC2390.4111403554041733No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGT2290.3939378300734548No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGT2240.3853365674080955No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCA2180.3750150522096644No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2150.36985429461044883No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGT2110.3629732844781614No Hit
ATCGGTAGGGGAGCGTTCTGTTGT1770.30448469835371833No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG1570.2700796476922812No Hit
CGCGACCTCAGATCAGACGT1420.2442758596962034No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT1380.237394849563916No Hit
ACTGGGTGCTGTACGCTT1320.22707333436548485No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC1260.21675181916705372No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCA1220.2098708090347663No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCT1110.1909480311709759No Hit
TTAGTGACGCGCATGAATG1090.1875075261048322No Hit
GTCTGCCTCAGCGTCGGT910.1565429805095388No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC910.1565429805095388No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGT890.1531024754433951No Hit
ACGAACTGGGAACAGCCT880.15138222291032324No Hit
CCCCGAGTGACGTAGTGT870.14966197037725137No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCG850.1462214653111077No Hit
CGCGACCTCAGATCAGACG840.14450121277803582No Hit
CGGTTGCTGAGCGTGTTT820.1410607077118921No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC820.1410607077118921No Hit
GTGCTACGATCTGCTGAGGTT760.13073919251346097No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAC740.12729868744731726No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCA700.12041767731502985No Hit
CACTGGGTGCTGTACGCTT690.118697424781958No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCA680.11697717224888612No Hit
ACACTGGGTGCTGTACGCTT630.10837590958352686No Hit
GCGGAGTCGTTGGCTGGGCT600.1032151519843113No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)