FastQCFastQC Report
Wed 30 Apr 2025
2D1-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2D1-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11470292
Total Bases292.3 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA416926936.348412054374904No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCT3302362.879054866257982No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCT1569861.3686312432150813No Hit
TGAAAATCTTTGCTCTGAAGTGGA1048900.9144492572638953No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC745340.649800371254716No Hit
TCAGGTCTAGGCTGGTTAGTTT415300.36206576083677733No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTT321340.28014979915071037No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT270790.23607943023595213No Hit
CTCGGGCTGAGACTTGAAGCGGGT255460.22271446969266342No Hit
TGAAAATCTTTTCTCTGAAGTGGAA253830.22129340735179193No Hit
TGAAAATCTTTGCTCTGAAGTGGGA244690.2133249964342669No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA240650.20980285419063435No Hit
TGAAAATCTTTGCTCTGAAGTGGTA232230.20246215179177654No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGC228030.198800518766218No Hit
TCCCGTAGATCCGAACTTGT227410.19825999198625457No Hit
AGCACTGATGACTGTTCAGTTTTTCTGAATT222770.19421475931039942No Hit
TATTAATGCTGTAGGTGAAAGACA218110.19015209028680352No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCC209840.18294216049600132No Hit
TCCGATGTCTGGTACCTTTGTGGTCTTGG208640.18189597963155602No Hit
TGAAAATCCTTGCTCTGAAGTGGAA200910.17515683122975423No Hit
CTCGGGCTGAGACTTGAAGCGG181660.15837434652927757No Hit
TGAAAATCTTTACTCTGAAGTGGAA178160.15532298567464542No Hit
GTGGAGCCGGCCTGGGCTG175040.15260291542708765No Hit
TGAAAATCTTTGCTCTGAGGTGGAA174800.15239367925419858No Hit
TGAAAATCTTTGCTCTGAAGTGG173380.15115569856460498No Hit
TTGTTTATGCCGTGAGGATTGGAGGAAAA169890.14811305588384324No Hit
TGAAAATCTTTGCTCTGAAGTGGAG167930.14640429380524925No Hit
GAGGTCCGGACGGAGGAGGGTTATCCAC166550.14520118581113714No Hit
TGAAAATCTTTGCTCTGAAGT166200.14489604972567394No Hit
CTGGCAACCTACCGCTAGTGGATGTGT164970.14382371433961752No Hit
GCTTAGGGTACTGTCGGCT161490.14078978983272616No Hit
TAAAATGTAAAATATGACGCTCCTAAAAGG154780.13493989516570284No Hit
TGTTTTACGGTGCGAAAACGATGTTCAAGT151370.1319669978759041No Hit
TGAAAATCTTTGCTCTGGAGTGGAA144930.12635249390338102No Hit
TCTCAGCTCACCAATCTCTGCT144080.12561144912439892No Hit
TGAAAATCTTTGCCCTGAAGTGGAA140380.12238572479235925No Hit
TCTCGGGCTGAGACTTGAAGCGGGT137180.11959590915383847No Hit
TGACGATCTTCCATTGTTCTTCGGAATGA134600.11734662029528106No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAA132280.11532400395735348No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCTG126480.11026746311253455No Hit
TCATACAATGGACAAGGATCAGG124040.10814022868816243No Hit
TAGTGATAGCGTCTTGGCTCAAAAAA121240.10569914000445674No Hit
CGAAAATCTTTGCTCTGAAGTGGAA119270.10398165975199236No Hit
CACCCCTCTTCCACTAACTTGACCTCATT115000.10025899950934117No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)