FastQCFastQC Report
Wed 30 Apr 2025
2B1-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2B1-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13670541
Total Bases369.2 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA227409316.63498906151556No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC3242022.3715374541504977No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGC2098411.5349868011807286No Hit
TCAGGTCTAGGCTGGTTAGTTT852480.6235890737608701No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC786440.5752808173429275No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC715850.5236442361717799No Hit
TGAAAATCTTTGCTCTGAAGTGGA564290.4127781043925036No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA554970.40596052489802703No Hit
TGAAAATCTTGGCTCTGAAGTGGAA522170.3819673266771227No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT427580.3127747468077525No Hit
TCCGCTGTAGTATAGTGGTTAGTATTTCCG417410.3053353923593806No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC390960.2859872187940477No Hit
TGAAAATCTTTGCTCTGAAGTGGGA388670.284312083918259No Hit
TCCGCTGTAGTATAGTGGTTAGTATTTCC355310.25990924572772944No Hit
TGGATAGATAAGACCGAAGATTTTCAATG353820.2588193108085481No Hit
TCCCGTAGATCCGAACTTGT301840.22079594362798077No Hit
TGAAAATCTTTGCTCTGAAGTGG277590.20305706994331826No Hit
TTGTAGTTGATTAAGTGGTAATAGCCAGGC257930.18867578100969085No Hit
CTCGGGCTGAGACTTGAAGCGGGT257830.18860263101511493No Hit
TGAAAACCTTTGCTCTGAAGTGGAA242040.1770522468715759No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGT237800.17395068710155656No Hit
TCCCGTAGATCCGAACTTGTGG237020.1733801171438643No Hit
TGAAAATCTTTGCTCTGAAGTGGGAA223810.16371700286038424No Hit
TTCAACGTGACAACCTGTATGGCTTCCATT206860.15131807877976447No Hit
CTCGGGCTGAGACTTGAAGCGG206800.1512741887830189No Hit
CCCAGGAGCATGTCTGTCTGAGTGTCGGA204840.1498404488893307No Hit
TCCGCTGTAGTATAGTGGTTAGTATTTC204780.14979655889258517No Hit
TGTTTGCACTGACTTCTGAAAATTGGAAGC199500.14593423917897616No Hit
GTAAGCTGCACAAAGTTCTCTGTGAATGTC175950.12870741545634515No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT174270.12747849554746957No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGC174260.12747118054801196No Hit
TATGTGGATCTGGATCTATGATCTGTTTGT169050.12366006583060611No Hit
GTGGAGCCGGCCTGGGCTG163840.11984895111320026No Hit
TTAGACGTTAGAAATTCTTGCCTCACACA160390.11732527630033075No Hit
TGAAAATCTTTGCTCTGAAGTGGTA151300.11067594179337893No Hit
TGGAAACGTCGTTGGTCTAAGTAGTGTT144610.10578220715624935No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGT140760.10296593236507612No Hit
TCCGCTGTAGTATAGTGGTTAGTATTTCCGC138870.10158339746759108No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)