FastQCFastQC Report
Wed 30 Apr 2025
2A3-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2A3-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84474
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTGATCGTATAGTGGTTAGTACTCTGC26313.1145677960082394No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT26163.0968108530435403No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC22702.687217368657812No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGT20832.4658474796978953No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT18172.1509576911238963No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGC11391.3483438691194924No Hit
GGCTGGTCCGAGTGCAGTGGTGCT10341.224045268366598No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGT7860.930463811350238No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC7530.8913985368278997No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCA6940.8215545611667495No Hit
CCCCCCACTGCTAGATTTGACT6890.8156355801785165No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCT6710.7943272486208774No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC5770.6830504060420958No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGC5250.6214930037644719No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGT4860.575324952056254No Hit
CGCGACCTCAGATCAGACGT4610.5457300471150887No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGA3840.45457773989629946No Hit
CCCCCACTGCTAGATTTGACT3830.4533939436986528No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC3650.43208561214101376No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGT3650.43208561214101376No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT3580.42379903875748753No Hit
TGAGATGAAGCACTGTAGCTC2920.34566848971281106No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAC2850.33738191632928477No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2690.3184411771669389No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCA2680.3172573809692923No Hit
GGCTGGTCCGAGTGCAGTGGTGCC2450.2900300684234202No Hit
CGGGCCGCCGGTGAAATACCACT2350.2781921064469541No Hit
GGCTGGTCCGAAGGTAGTGAGT2310.27345692165636765No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC2230.2639865520751948No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG2130.2521485900987286No Hit
AACCCGTAGATCCGATCTTGT2080.24622960911049552No Hit
TGGACGGAGAACTGATAAGGGT2080.24622960911049552No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG1880.22255368515756327No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGC1800.21308331557639038No Hit
TGAGATGAAGCACTGTAGCT1780.21071572318109716No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCA1700.20124535359992424No Hit
GGCTGGTCCGAGTGCAGTGGTGC1600.1894073916234581No Hit
CCCTGTGGTCTAGTGGTTAGGATTCGGC1580.18703979922816488No Hit
TGAGATGAAGCACTGTAGCTT1520.1799370220422852No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGA1490.17638563344934535No Hit
CCCCCCACTGCTAGATTTGACC1490.17638563344934535No Hit
GGCTGGTCCGAGTGCAGTGGTGT1490.17638563344934535No Hit
CGCGACCTCAGATCAGACG1450.1716504486587589No Hit
GGCTGGTCCGAGTGCAGTGGTGCA1430.1692828562634657No Hit
TTAGTGACGCGCATGAATG1120.13258517413642065No Hit
ACTGGGTGCTGTACGCTT1110.13140137793877407No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCA1110.13140137793877407No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGT1080.12784998934583422No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCG1060.125482396950541No Hit
CCCTGTGGTCTAGTGGTTAGGATTCGGT1030.12193100835760115No Hit
CGCGACCTCAGATCAGAC1030.12193100835760115No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC970.11482823117172147No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGA940.11127684257878163No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCT900.10654165778819519No Hit
ATCGGTAGGGGAGCGTTCTGTTGT890.10535786159054858No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)