FastQCFastQC Report
Wed 30 Apr 2025
1H3-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1H3-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences896907
Total Bases23.3 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGCTTAAGCGTGAGTAGTTGTCTGCT12385313.808901034332433No Hit
TGAAAATCTTTGCTCTGAAGTGGAA354223.949350378578827No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCT324263.615313516340044No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCC133501.488448635142774No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC104611.1663416608410906No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGC52480.5851219803167999No Hit
ATGTACAATTCTAGACTCTTTGGTGGT51880.5784323235296414No Hit
GTATTGTAAGTGCTGAGAGT46150.5145461012122773No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGC40490.4514403388534151No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT34680.38666216229776335No Hit
GTGTGCTCAGGATCTGTGACGTTTACTTT31100.34674721013438403No Hit
TGGGAATACCCCGTGTTGTAGGCTT28660.31954260586660604No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCC26900.2999196126242743No Hit
CACCCCTCTTCCACTAACTTGACCTCAT22910.25543339498967005No Hit
GCTTAGGGTACTGTCGGCT21780.24283454137385482No Hit
CATCTTACATGTATGTTTGAACTCATATGT20500.22856327356124995No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTT19720.21986671973794383No Hit
GAGGTCCGGACGGAGGAGGGTTATCCAC19160.21362304006992922No Hit
ATTAGTGTTGATGATGGAATTTATGAAGGCT19130.2132885572305713No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA18840.21005522311677802No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCA18170.20258510637111765No Hit
GATTGCTGTAGGCTGCTATGAGCCTTGGGC17850.1990172894179664No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCTG17670.19701039238181886No Hit
TGAAAATCTTTGCTCTGAAGTGGA17560.1957839553041731No Hit
GGGGACTAGTTGGGAAGT16810.18742188432022494No Hit
CACCCCTCTTCCACTAACTTGACCTCATT16600.18508050444471946No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAA13450.14995980631213715No Hit
ATGTACAATTCTAGACTCTTTGGT13420.14962532347277924No Hit
GTGTCTTCACGAGCTTGCTCTGGA13180.14694946075791582No Hit
ATTGTGAGGTGTGTCTTTTCTTAAAGC12480.1391448611728975No Hit
TAAAAAACTCGTCGACGTG12450.1388103783335396No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCTGT12080.1346850899814585No Hit
GTTCTTCTTCGCAAAGACTGTGTGTGCT11980.13357014718359875No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT11950.1332356643442408No Hit
GAGTGTGCTCAGGATCTGTGACGTTTACTTT11680.13022531879001947No Hit
AGTGTGCTCAGGATCTGTGACGTTTACTTT11630.1296678473910896No Hit
GAGGTCCGGAACTCAGGGGAGGGTTATC11600.12933336455173167No Hit
ATAAAGAACGGGGTATTGTAAGTTGTAGAGT10780.12019083360928168No Hit
TTGTTTATGCCGTGAGGATTGGAGGAAAA10720.11952186793056582No Hit
GAGGTCCGGACGGTTGAGGGTTATC10320.1150620967391268No Hit
GCGTGTTGTCTTCGACCTATTCTCAAACTT10130.11294370542319326No Hit
TCTTCTTCGCAAAGACTGTGTGTGCT9960.11104830266683169No Hit
AATTGTGAGGTGTGTCTTTTCTTAAAGC9740.1085954285115402No Hit
CATCTTCCTGAAGAACGTATCTGCA9640.10748048571368046No Hit
GTGAAATTCTTGGATTTGTTAAAGACGG9570.10670002575517863No Hit
AGGTATTGTAAGTGCTGAGAGT9480.10569657723710484No Hit
GTGTGCTCAGGATCTGTGACGTTTACT9280.10346669164138533No Hit
CACTAACTTGACCTCATT9160.10212876028395364No Hit
AATATTATGTTGACGTGCATTATCTGACT9150.10201726600416766No Hit
GTGAAATTCTTGGATTTGTTAAAGACG8970.1000103689680201No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)