FastQCFastQC Report
Wed 30 Apr 2025
1H2-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1H2-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541044
Total Bases13.8 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA6371511.776306548081116No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT68861.2727245843221622No Hit
CACCCCTCTTCCACTAACTTGACCTCAT33880.6261967603374217No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC30810.5694546099762681No Hit
CACCCCTCTTCCACTAACTTGACCTCATT29850.551711136247699No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAA28030.5180724673039531No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA26730.49404484662984893No Hit
CTGGCAACCTACCGCTAGTGGATGTGT25040.4628089397535136No Hit
TATTAATGCTGTAGGTGAAAGACA22520.4162323212160194No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTA22330.41272059204057343No Hit
GAGGTCCGGATTTGGGGAGGGTTATCTACT21410.3957164297173612No Hit
GATCGGCTCATTAATTCATGGA19960.36891639127316816No Hit
TGAAAATCTTTGCTCTGAAGTGGA19190.35468464672004496No Hit
CTCGGGCTGAGACTTGAAGCGGGT16590.3066294053718367No Hit
GTGTGCTCAGGATCTGTGACGTTTACTTT16460.3042266433044262No Hit
AGCACTGATGACTGTTCAGTTTTTCTGAATT15210.2811231618870184No Hit
TTGTTTATGCCGTGAGGATTGGAGGAAAA14770.27299073642809085No Hit
TCCCGTAGATCCGAACTTGT13030.24083069029505916No Hit
TCTCGGGCTGAGACTTGAAGCGGGT12400.22918653566068561No Hit
GAGGTCCGGACGGAGGAGGGTTATCCAC11360.20996443912140234No Hit
TATAGACTTGGAAGCGGGAAAAAAAAAAA10110.1868609577039945No Hit
CAATGTTTCGGCTTGTTCCCG9760.1803919829071203No Hit
TTCTGTGGCGTGAGTGAGT9720.17965267150176326No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT9650.1783588765423884No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTT9650.1783588765423884No Hit
CACTAACTTGACCTCATT9550.1765105980289958No Hit
CGAGTAGCGATTAGTGAATT9150.16911748397542528No Hit
GCGTGTTGTCTTCGACCTATTCTCAAACTT9010.16652989405667562No Hit
CACCCCTCCTCCAAATACTTGACCTCTT9000.16634506620533634No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTA8960.1656057547999793No Hit
TATAGACTTGGAAGCGGGAAAAAAAAAAAA8760.16190919777319407No Hit
GTGTGCCGAAGAATCTCTGTTATTT8610.1591367800031051No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCT8090.14952573173346345No Hit
GAGGTCCGGAACTCAGGGGAGGGTTATC8070.14915607603078493No Hit
CTCGGGCTGAGACTTGAAGCGG7680.1419477898285537No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAA7650.14139330627453592No Hit
GTATTGTAAGTGCTGAGAGT7580.14009951131516107No Hit
CCAACTCCGACAGCATAGGTGTGCGATGT7010.1295643237888231No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCT6830.12623742246471636No Hit
GTACGTGATGAATTCTCAACTTCAATGGT6740.124573971802663No Hit
TAAAAAACTCGTCGACGTG6740.124573971802663No Hit
TAGTGATAGCGTCTTGGCTCAAAAAA6730.12438914395132375No Hit
GAGGTCCGGACGGAGGAGGGTTAT6660.1230953489919489No Hit
CATCTTCCTGAAAAACGTATCTGCA6490.11995327551918143No Hit
GTGTGCCGAAGAATCTCTGTTCTTTGAAGT6440.11902913626248511No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACG6420.11865948055980659No Hit
TCAGGTCTAGGCTGGTTAGTTT6400.11828982485712806No Hit
TTAGACGTTAGAAATTCTTGCCTCACACA6350.11736568560043174No Hit
TGAAAATCTTTGCTCTGAAGT6290.11625671849239617No Hit
GAGTGTGCTCAGGATCTGTGACGTTTACTTT6210.11477809568168208No Hit
CCCCCCTCCCCGCTCTTAGACCTCACT6090.11256016146561093No Hit
CGGGCTGAGACTTGAAGCGGGT6040.11163602220891462No Hit
ATGTACAATTCTAGACTCTTTGGTGGT5920.10941808799284347No Hit
AGTGTGCTCAGGATCTGTGACGTTTACTTT5880.1086787765874864No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACC5860.10830912088480787No Hit
TATAGACTTGGAAGCGGGAAAAAAAAAAAAA5770.10664567022275454No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)