FastQCFastQC Report
Wed 30 Apr 2025
1H1-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1H1-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1579947
Total Bases41 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA1535629.719439955897254No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCT400832.5369838355337238No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCT189221.1976351105448475No Hit
CACCCCTCTTCCACTAACTTGACCTCAT96760.6124256066817432No Hit
CACCCCTCTTCCACTAACTTGACCTCATT82750.5237517461028756No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCC76910.48678848087942195No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA68540.4338120202766295No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAA66090.4183051709962423No Hit
GTGTGCTCAGGATCTGTGACGTTTACTTT62180.39355750540999157No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC59550.3769113774069637No Hit
TATTAATGCTGTAGGTGAAAGACA53260.3370999153769082No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT50510.31969426822545316No Hit
TGGGAATACCCCGTGTTGTAGGCTT49960.3162131387951621No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT45490.2879210505162515No Hit
TGAAAATCTTTGCTCTGAAGTGGA42300.26773049982056357No Hit
TCCCGTAGATCCGAACTTGT40980.25937578918786514No Hit
TAAAATGTAAAATATGACGCTCCTAAAAGG38340.2426663679224683No Hit
TTCTGTGGCGTGAGTGAGT37570.23779278672006088No Hit
GTGTGCTCAGGATCTGTGACGTTTACT34690.21956432715780974No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCC33570.21247548177248982No Hit
AGTGTGCTCAGGATCTGTGACGTTTACTTT32710.20703226120876206No Hit
CTCGGGCTGAGACTTGAAGCGGGT29440.18633536441412274No Hit
CGAAAGACGAACTACTGCGAAAGCATT27950.17690466832115254No Hit
AGCACTGATGACTGTTCAGTTTTTCTGAATT26790.16956264988635694No Hit
TCTTCTTCGCAAAGACTGTGTGTGCT24920.15772680982336748No Hit
TTGTTTATGCCGTGAGGATTGGAGGAAAA24510.15513178606624145No Hit
CTCCCCGCTCTTTGACCTCAT22590.14297947969140737No Hit
ATGTACAATTCTAGACTCTTTGGTGGT22510.14247313359245595No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTT21890.13854895132558245No Hit
CATCTTCCTGAAGAACGTATCTGCA21210.1342450094844954No Hit
CACCCCTCCTCCAAATACTTGACCTCTT20740.13127022615315578No Hit
TTAGACGTTAGAAATTCTTGCCTCACACA20110.12728275062391334No Hit
GCTAAGGAGTGTGTAACAACT20030.12677640452496192No Hit
GCGTGTTGTCTTCGACCTATTCTCAAACTT19930.12614347190127265No Hit
ATCACCCCTCTTCCACTAACTTGACCTCATT19750.12500419317863196No Hit
CTGGCAACCTACCGCTAGTGGATGTGT19500.12342186161940875No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCA19390.12272563573335056No Hit
GTTCTTCTTCGCAAAGACTGTGTGTGCT18660.11810522758041885No Hit
GTGTGCTCAGGATCTGTGACGTT18040.11418104531354532No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAA17810.11272530027906No Hit
TCCGATGTCTGGTACCTTTGTGGTCTTGG17700.1120290743930018No Hit
AAGACGAACTACTGCGAAAGCATT17650.11171260808115716No Hit
TCTCGGGCTGAGACTTGAAGCGGGT17400.11013027652193397No Hit
TATAGACTTGGAAGCGGGAAAAAAAAAAA17340.1097505169477204No Hit
GAGTGTGCTCAGGATCTGTGACGTTTACTTT17260.10924417084876897No Hit
AAAGACGAACTACTGCGAAAGCATT16880.10683902687874973No Hit
CACCCCTCTTCCACTAACTTGACCTCATTT16640.10531998858189548No Hit
CGAAAGACGAACTACTGCGAAAGCAT16490.10437058964636156No Hit
CTCCCCGCTCTTTGACCTCATT16260.10291484461187622No Hit
GAAAGACGAACTACTGCGAAAGCATT15980.10114263326554626No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)