FastQCFastQC Report
Wed 30 Apr 2025
1G3-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1G3-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences840423
Total Bases21.2 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA13211615.720179005096243No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGC92221.097304571626431No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC90911.081717182894804No Hit
CTCGGGCTGAGACTTGAAGCGGGT58040.6906046122012368No Hit
TCTCGGGCTGAGACTTGAAGCGGGT45900.5461535441081455No Hit
TATTAATGCTGTAGGTGAAAGACA44780.5328269216811058No Hit
CATCACGAATTTCCCTGCTCT39280.4673836865483215No Hit
TGAAAATCTTTGCTCTGAAGTGGA36550.4349000443824122No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAA35540.4228822866580282No Hit
CTCGGGCTGAGACTTGAAGCGG33810.4022974145162615No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA30460.3624365349353837No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT26140.31103384843108767No Hit
CACCCCTCTTCCACTAACTTGACCTCAT18880.2246487780558124No Hit
TCCGATGTCTGGTACCTTTGTGGTCTTGG18070.21501077433625687No Hit
CGGGCTGAGACTTGAAGCGGGT17810.21191709412997978No Hit
GATCGGCTCATTAATTCATGGA17310.2059677091179085No Hit
TTGTTTATGCCGTGAGGATTGGAGGAAAA16550.1969246438995601No Hit
TAAAAAACTCGTCGACGTG15720.18704866477952173No Hit
CACCCCTCTTCCACTAACTTGACCTCATT15150.18026636586576045No Hit
CTGGCAACCTACCGCTAGTGGATGTGT14850.17669673485851767No Hit
GAGGTCCGGACGGAGGAGGGTTATCCAC14640.17419799315344772No Hit
TCCCGTAGATCCGAACTTGT13460.16015744452495945No Hit
CATCTTCCTGAAAAACGTATCTGCA12900.1534941333114396No Hit
CGGTTGTGGCGGTCCGGGGCTTGCGGATC12240.1456409450955055No Hit
TAGTGATAGCGTCTTGGCTCAAAAAA12090.1438561295918841No Hit
CACTAACTTGACCTCATT11740.13969156008343417No Hit
TGAAAATCTTTGCTCTGAAGTGG11510.13695484297788138No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTT11190.13314723657015573No Hit
CTCGGGCTGAGACTTGAAGCG11180.13302824886991432No Hit
GAGGTCCGGATTTGGGGAGGGTTATCTACT10990.13076748256532722No Hit
TCTCGGGCTGAGACTTGAAGCGG10660.12684088845736016No Hit
CTCGGGCTGAGACTTGAAGCGGGTG10610.12624594995615301No Hit
TCATACAATGGACAAGGATCAGG10500.12493708525349735No Hit
TAAAAAACTCGTCGACGTGA10360.12327125745011738No Hit
GAGGTCCGGACGGTTGAGGGTTATC9750.1160130077353904No Hit
AATTGTGAGGTGTGTCTTTTCTTAAAGC9600.11422819223176901No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAA9540.11351426603032044No Hit
TGAAAATCTTTGCTCTGAAGTGGTA9380.11161046282645763No Hit
GCCCGGCCGGTCTGCCGCAAGGTATGTTACT9090.10815981951945627No Hit
TGAAAATCTTTTCTCTGAAGTGGAA9090.10815981951945627No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC9010.10720791791752488No Hit
GCGTGTTGTCTTCGACCTATTCTCAAACTT8610.10244840990786781No Hit
CTTGATGAAGAAATGCTATGTAAGCTTCACC8530.10149650830593641No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)