FastQCFastQC Report
Wed 30 Apr 2025
1G10-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1G10-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30906172
Total Bases899.8 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC743158624.045637227412055No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT634509020.530171125689716No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC11486423.716545678966648No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT10184193.2951961828206997No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA6525632.111432629055452No Hit
TGAAAATCTTTGCTCTGAAGTGGAA3057650.9893331338478282No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGC2677300.8662671003060489No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGT1735860.5616548047425608No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC1094960.35428522173499843No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA1025150.3316975004215986No Hit
ACACTGGTGGTTCAGTGGTAGAATTCTCGCC990790.32057997994704746No Hit
ACACTGGTGGTTCAGTGGTAGAATTCTCGCT975320.3155745072537615No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCG814150.263426347332824No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT781320.25280387360815826No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTT767930.24847140564674264No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGT720550.2331411343986567No Hit
CTCGGGCTGAGACTTGAAGCGGGT663150.214568792278772No Hit
GCACTTGTGGTTCAGTGGTAGAATTCTCGCC660090.21357869877900115No Hit
TCAGGTCTAGGCTGGTTAGTTT633790.20506907164044774No Hit
GCACTTGTGGTTCAGTGGTAGAATTCTCGCT582430.18845103172272515No Hit
ATAAAGAACGGGGTATTGTAAGTTGTAGAGT503690.16297391990182414No Hit
AGAACGGGGTATTGTAAGTTGTAGAGT480000.15530878427778116No Hit
CGGGCTGAGACTTGAAGCGGGT444640.1438677038359846No Hit
GCACTAGTGGTTCAGTGGTAGAATTCTCGCC427980.1384771947816766No Hit
TCTCAGCTCACCAATCTCTGCT424500.1373512060956627No Hit
ATGTACAATTCTAGACTCTTTGGTGGT404880.1310029595383084No Hit
GCACTGGTGGTTCAGTGGTAGGATTCTCGCC393060.12717848072546803No Hit
GCACTAGTGGTTCAGTGGTAGAATTCTCGCT384180.12430526821632909No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC375200.1213996997104656No Hit
GCACTGGTGGTTCAGTGGTAGGATTCTCGCT360110.11651717980473285No Hit
ACGATCTTCTGAGATTAAGCCTTTGTT345640.11183526707869225No Hit
GTATTGTAAGTGCTGAGAGT324800.10509227736129859No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)