FastQCFastQC Report
Wed 30 Apr 2025
1G1-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1G1-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29950049
Total Bases837.6 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA17031385.686595036956367No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGC5505761.8383141877330484No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC3961101.3225687877839531No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT3527991.1779580060119434No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT3158311.0545258206422299No Hit
TCAGGTCTAGGCTGGTTAGTTT2932770.9792204346643975No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGT2631820.878736458828498No Hit
TGACCACTTTGGATTCTTGCGCCATTCTT1905030.6360690762141992No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTT1576690.5264398732703242No Hit
TCCCGTAGATCCGAACTTGT1425690.47602259348557324No Hit
TGATTAGATCTAATGGTTTTTGAGCAAATT996310.3326572186910278No Hit
GAGGTCCGGACGGAGGAGGGTTATCCAC869820.2904235649163713No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC798150.26649372092846996No Hit
TAAAATGTAAAATATGACGCTCCTAAAAGG705940.2357057913327621No Hit
GAGGTCCGGATTTGGGGAGGGTTATCTACT699000.23338859979828414No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT643310.2147943063465439No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACC638490.21318496006467302No Hit
TTTGCTCTTTCAACACTTCTAACTTTGT600220.2004070176980345No Hit
TTCAACGTGACAACCTGTATGGCTTCCATT599510.2001699563162651No Hit
TCAAGTCTAGGCTGGTTAGTTT593170.19805309834384577No Hit
TGAGACAATGCACATGGTAAAACAA480990.1605974000242871No Hit
TCAGGTCTAGGCTGGTTAGTTC477590.15946217650595496No Hit
TGAAAATCTTTGCTCTGAAGTGGA464600.155124954887386No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTA442520.14775267980362902No Hit
TGAAAATCTTTGCTCTGAAGTGGAT417450.13938207580228No Hit
TTAGACGTTAGAAATTCTTGCCTCACACA408230.1363036167319793No Hit
TAGTAAACCGAGCTGCATTTAATAACAAAC400790.1338194805624525No Hit
TGCATACGGTCCGGTCTCTAATGGCTTCT355710.1187677522664487No Hit
TCAGGTCTAGGCTGGTTAGTT353190.11792635130580253No Hit
AGCTGTCGACTCCTGCACCAAGAAGAGTGT346040.11553904302460406No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA343880.11481784220119305No Hit
CACCCCTCTTCCACTAACTTGACCTCAT323830.10812336233573441No Hit
ACACGATCAATTGTCCGAAGCAACTGCAAT305500.10200317201484378No Hit
GAGGTCCGGACGGAGGAGGGTTAT303700.10140217132866794No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)