FastQCFastQC Report
Wed 30 Apr 2025
1F7-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1F7-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2090363
Total Bases55.1 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGCTTAAGCGTGAGTAGTTGTCTGCT27878713.3367745219371No Hit
TGAAAATCTTTGCTCTGAAGTGGAA2060599.857570192354151No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCT1643857.86394516167766No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCC469902.247934928048382No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCC238001.138558231273707No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGC126900.6070715947421572No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGC111870.5351702072797883No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT107030.5120163340051465No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCA98440.4709229928007719No Hit
TGAAAATCTTTGCTCTGAAGTGGA71650.3427634339107609No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGT71540.34223720951815545No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC64730.3096591357577607No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA59650.28535713653561606No Hit
CACCCCTCTTCCACTAACTTGACCTCATT54640.2613900073814931No Hit
TGAAAATCTTTTCTCTGAAGTGGAA49170.23522230349465617No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGT47680.22809435490390903No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTT46240.22120559921889166No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAA45900.21957908745992924No Hit
CACCCCTCTTCCACTAACTTGACCTCAT43010.20575373750874848No Hit
GCGTGTTGTCTTCGACCTATTCTCAAACTT42170.20173529669248835No Hit
ATGTACAATTCTAGACTCTTTGGTGGT39790.19034971437975126No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT39770.19025403721745934No Hit
TCCCGTAGATCCGAACTTGT35860.1715491519893913No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCA35200.16839180563375833No Hit
TCAGGTCTAGGCTGGTTAGTTT34440.16475607346666585No Hit
TATTAATGCTGTAGGTGAAAGACA29830.14270248755838102No Hit
TGAAAATCTTTGCTCTGAAGT29450.14088462147483477No Hit
GTGTGCTTAAGCGTGAGTAGTT25670.12280163780166412No Hit
TGAAAATTTTTGCTCTGAAGTGGAA25300.12103161029926382No Hit
TTGTTTATGCCGTGAGGATTGGAGGAAAA23850.11409501603310047No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCTGT23290.11141605548892705No Hit
GTGTGCTCAGGATCTGTGACGTTTACTTT23110.11055496102829988No Hit
CACTAACTTGACCTCATT21710.1038575596678663No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCTG21160.10122643770483883No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)