FastQCFastQC Report
Wed 30 Apr 2025
1F6-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1F6-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13238685
Total Bases357.4 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA157151611.870635187709354No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC2417671.8262161234291776No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGC729890.5513311933926972No Hit
TCATACAATGGACAAGGATCAGG552790.4175565775603846No Hit
TGATTAGATCTAATGGTTTTTGAGCAAATT543180.4102975484347577No Hit
TCAGGTCTAGGCTGGTTAGTTT541030.4086735200663812No Hit
TGAAAATCTTTGCTCTGAAGTGGAG419540.31690458682263384No Hit
CCCAGGAGCATGTCTGTCTGAGTGTCGGA409620.30941139546714796No Hit
TGAAAATCTTTGCTCTGAAGTGGA393300.2970838871081229No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC366170.2765909151853073No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT347150.2622239293404141No Hit
TGAAAATCTTTGCTCTGAAGTGGTA329900.24919393429181222No Hit
TTCAACGTGACAACCTGTATGGCTTCCATT317750.24001628560540567No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA294870.22273360231775283No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC287310.2170230653573221No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT283120.2138580984440675No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTT265870.20082810339546567No Hit
TCCCGTAGATCCGAACTTGT264680.19992922257762005No Hit
TGAAAATCTTTGCTCTGAAGTGGGA255290.19283637309898982No Hit
GAGGTCCGGACGGAGGAGGGTTATCCAC247700.18710317527760498No Hit
TGAAAATCTTTGCTCTGAAGTGG233990.17674716182158576No Hit
TGAGATTAAGCCTTTGTTCTAAGATTTGT228970.17295524442193466No Hit
TATCAGCATCTGTTCAGCATCTGACGTGT225510.17034169179189626No Hit
CATGTCTGTCTGAGTGTCGGA224690.16972229492581778No Hit
TCCCGTAGATCCGAACTTGTGG222010.16769792468058572No Hit
TGAAAATCTTTGCTCTGAAGTGAA198230.1497354155643102No Hit
CCTTTGTTCTAAGATTTGT186540.14090523341253303No Hit
TGATCTTCATTGTGTTTATCGGACTCAACA183950.13894884575016325No Hit
CTGAGATTAAGCCTTTGTTCTAAGATTTGT183880.13889597040793705No Hit
CTCCCAGGAGCATGTCTGTCTGAGTGTCGGA173310.13091179373177925No Hit
ATGTCTGTCTGAGTGTCGGA170730.12896295968972749No Hit
TTGTAGTTGATTAAGTGGTAATAGCCAGGC166560.12581310001710894No Hit
TTGACCTCAGATCAGGCA163450.12346392409820159No Hit
GCATGTCTGTCTGAGTGTCGGA162480.12273122292735267No Hit
CTTTGTTCTAAGATTTGT154450.11666566581197452No Hit
TGAAAATCTTTGCTCTGAAGTGGGAA152860.11546464018140776No Hit
TCCCAGGAGCATGTCTGTCTGAGTGTCGGA149550.1129643918561398No Hit
TGGGAATACCCCGTGTTGTAGGCTT145490.10989762200701958No Hit
TCTGAGATTAAGCCTTTGTTCTAAGATTTGT144040.10880234706090522No Hit
TTACCGTGACCGATCCCGT143000.1080167705478301No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT136650.10322022164588099No Hit
TGTCTGTCTGAGTGTCGGA133340.10071997332061304No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)