FastQCFastQC Report
Wed 30 Apr 2025
1F3-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1F3-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31683194
Total Bases845.7 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA490216515.472445738898674No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCT13909484.3901760662135265No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCT12619873.9831432399145115No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGC4960601.5656881058140792No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC4255481.3431347862213638No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCC2627980.8294555151226229No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT2305190.7275750039595125No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCC1927020.6082151944655579No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGT1784470.5632228871874471No Hit
TGAAAATCTTTGCTCTGAAGTGGA1451290.4580630349326523No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT1429420.4511603217781642No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA1233540.3893357468947102No Hit
TCCCGTAGATCCGAACTTGT1067650.3369767580882155No Hit
CACCCCTCTTCCACTAACTTGACCTCAT1041420.3286979210492477No Hit
TGAAAATCTTTTCTCTGAAGTGGAA1039500.32809192154048605No Hit
CACCCCTCTTCCACTAACTTGACCTCATT1032230.32579732965053965No Hit
TCAGGTCTAGGCTGGTTAGTTT908870.28686186121260376No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTT848960.26795278279077545No Hit
AGCTGTCGACTCCTGCACCAAGAAGAGTGT630980.19915290106167957No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGC604090.19066575169157504No Hit
TGAAAATCTTTGCTCTGAAGTGGGA589640.18610497413865534No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCA529670.16717695823217824No Hit
TGAAAATCTTTGCTCTGAAGT525690.1659207717504744No Hit
TGAAAATCTTTGCTCTGAAGTGG500760.15805224687889738No Hit
TCCGATGTCTGGTACCTTTGTGGTCTTGG480020.15150618968529497No Hit
TGAAAATCTTTGCTCTGAAGTGGTA465870.1470400995556193No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGC454440.1434325087300226No Hit
CACCCCTCCTCCAAATACTTGACCTCTT418320.1320321429714441No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC405420.12796058377195177No Hit
TGATTAGATCTAATGGTTTTTGAGCAAATT398290.1257101793461859No Hit
TGAAAATTTTTGCTCTGAAGTGGAA393780.1242867117500843No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAA379300.11971646545484019No Hit
TGAAAATCTTTGCTCTGAAGTGGAT370690.11699893640773718No Hit
TCCGATTTGCGATGGCTTTAGAGCGAAGT363340.11467909453825899No Hit
TTGTAGTTGATTAAGTGGTAATAGCCAGGC362280.1143445323094635No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCTG351250.11086319138152549No Hit
TGAAAATCTTTCCTCTGAAGTGGAA341020.10763435024890482No Hit
TCTCAGCTCACCAATCTCTGCT337630.10656438236624755No Hit
TTAGACGTTAGAAATTCTTGCCTCACACA319290.10077582455859722No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)