FastQCFastQC Report
Wed 30 Apr 2025
1F12-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1F12-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32227095
Total Bases900.4 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA15495244.808140479307862No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC11747813.6453208084687745No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT10263153.1846339237216386No Hit
TCAGGTCTAGGCTGGTTAGTTT3900401.210285941069153No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC2522310.7826675038504091No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGC2477860.7688747620596893No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT2245680.6968297949287703No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT2170740.6735760700739548No Hit
TGACCACTTTGGATTCTTGCGCCATTCTT1816240.5635754634415544No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGT1459590.4529077163175893No Hit
TCCCGTAGATCCGAACTTGT1441450.44727891235620215No Hit
TGCTGTCTATTCCTGCACCTGAAAGAGATT1080330.3352241336055887No Hit
TCTCAGCTCACCAATCTCTGCT1069400.331832577525216No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA958080.29729021495732083No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC860400.266980315787073No Hit
TCAAGTCTAGGCTGGTTAGTTT839120.2603771764100984No Hit
TCCGCTGTAGTATAGTGGTTAGTATTTCC766930.23797677078867951No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTT703290.21822941223836653No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGT643220.19958981720195382No Hit
GAGGTCCGGATTTGGGGAGGGTTATCTACT639840.19854101028963358No Hit
TGGAAACGTCGTTGGTCTAAGTAGTGTT636940.197641146370779No Hit
TTCAACGTGACAACCTGTATGGCTTCCATT616520.19130486319043027No Hit
TATGTGGATCTGGATCTATGATCTGTTTGT600380.18629665503514975No Hit
TCCGCTGTAGTATAGTGGTTAGTATTTC587410.18227209123254826No Hit
TGCTGTCTATTCCTGCACCCGGAAGAGGAT573990.17810789337357275No Hit
TCAGGTCTAGGCTGGTTAGTTC569520.17672086174692445No Hit
TCAGGTCTAGGCTGGTTAGTT561470.17422296362734524No Hit
TCCTGATATTCCGATTTGTTATTACGTC519110.1610787444540068No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC495710.15381777352255918No Hit
CACCCCTCTTCCACTAACTTGACCTCAT448010.13901656354691605No Hit
TCCTGATATTCCGATTTGTTATTACGTCT422950.13124049809639993No Hit
TGAAAATCTTTGCTCTGAAGTGGA415740.12900325021538553No Hit
TGAAAATCTTGGCTCTGAAGTGGAA397430.12332169561047931No Hit
TCCGCTGTAGTATAGTGGTTAGTATTTCCGC390230.12108755070849544No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACC387750.12031801190892323No Hit
GAGGTCCGGACGGAGGAGGGTTATCCAC380160.11796285082474856No Hit
TGCTGTCTATTCCTGTACTCGGAAGAGGAT376160.11672165921253529No Hit
CACCCCTCTTCCACTAACTTGACCTCATT369450.11463956028304753No Hit
TGCCAGTACATGTAGATTGAAGGGAAGAGT359360.11150865444123957No Hit
TGTACGATGTATGGCCTGACTTTTTGAGT342610.10631116456509655No Hit
TGCTGTCTACTCCTGCACAAAGAGGATTGT342340.10622738413127214No Hit
ACACGATCAATTGTCCGAAGCAACTGCAAT336690.10447420097902092No Hit
TCCGCTGTAGTATAGTGGTTAGTATTTCT324330.10063891889728192No Hit
TTAGACGTTAGAAATTCTTGCCTCACACA324160.10058616825376286No Hit
TACAGATGGTGTAATTCTTGCTGCCTAGGC322370.10003073500729742No Hit
TACTGACGTGCGTTGTACCGATAGCCGA322290.10000591117505317No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)