FastQCFastQC Report
Wed 30 Apr 2025
1F10-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1F10-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4240274
Total Bases120 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA1384443.2649776877626304No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC1300113.0660990303928473No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT1287193.035629301314019No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT637811.5041716643782925No Hit
TCAGGTCTAGGCTGGTTAGTTT273320.6445809869833883No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC269870.6364447203176021No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT266970.6296055396420137No Hit
TGACCACTTTGGATTCTTGCGCCATTCTT255380.6022724003213No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTT228750.5394698550140863No Hit
GAGGTCCGGATTTGGGGAGGGTTATCTACT214350.5055097854525439No Hit
GAGGTCCGGACGGAGGAGGGTTATCCAC166060.39162563551317675No Hit
TGGAAACGTCGTTGGTCTAAGTAGTGTT149300.35209988788460367No Hit
TCCCGTAGATCCGAACTTGT130780.30842346508739765No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACC116980.27587839842425277No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA113770.26830813291782557No Hit
CACCCCTCTTCCACTAACTTGACCTCAT95010.22406570896126052No Hit
TTCCAGTCAACTTTGTATCTGTCCCAGAGT83990.19807682239402452No Hit
TTCATGATCCACGAACGTTTTCTTCTAAGT80230.18920947089739956No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACG79370.187181300076363No Hit
GAGGTCCGGACGGAGGAGGGTTATCCAT69540.16399883592428224No Hit
GAGGTCCGGACGGAGGAGGGTTAT68550.1616640811419262No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC67690.15963591032088964No Hit
TTCAACGTGACAACCTGTATGGCTTCCATT67610.15944724326776996No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCT66160.15602765292997575No Hit
TCAAGTCTAGGCTGGTTAGTTT64650.1524665623023418No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGC61900.14598113235135277No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTA56200.13253860481657553No Hit
CACCCCTCTTCCACTAACTTGACCTCATT52370.12350616964847082No Hit
GAGGTCCGGACGGTTGAGGGTTATCATACT51130.1205818303251158No Hit
TGATTAGATCTAATGGTTTTTGAGCAAATT49740.11730374027716134No Hit
TGAAAATCTTTGCTCTGAAGCGGAA49640.11706790646076175No Hit
TAACCTGTGCCACCTAAACTATGACTCGAAT46530.10973347477073415No Hit
GCCGGTCTTAAGAAAATTCGAAGAGTACT46450.10954480771761448No Hit
TCTCAGCTCACCAATCTCTGCT45940.10834205525397651No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT45770.10794113776609719No Hit
TTGGAAGGGATTTGAATT45740.10787038762117732No Hit
AGCTGTCGACTCCTGCACCAAGAAGAGTGT44490.10492246491618232No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC44320.104521547428303No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTA42850.10105479032722885No Hit
TCAGGTCTAGGCTGGTTAGTTC42680.10065387283934953No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)