FastQCFastQC Report
Wed 30 Apr 2025
1F1-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1F1-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189542
Total Bases5 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA168858.90831583501282No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC53422.8183727089510504No Hit
CTCGGGCTGAGACTTGAAGCGGGT25641.3527344862879995No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT23251.226641061084087No Hit
TCTCGGGCTGAGACTTGAAGCGGGT19471.0272129659917064No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT18320.9665403973789449No Hit
AGCACTGATGACTGTTCAGTTTTTCTGAATT13080.6900845195260153No Hit
CACCCCTCTTCCACTAACTTGACCTCAT12610.6652879045277563No Hit
CTCGGGCTGAGACTTGAAGCGG12410.6547361534646675No Hit
CACCCCTCTTCCACTAACTTGACCTCATT8760.4621666965632947No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTA7420.39146996444059895No Hit
TCCCGTAGATCCGAACTTGT6580.34715260997562547No Hit
GAGGTCCGGACGGAGGAGGGTTATCCAC5930.31285941902058645No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTT5560.29333867955387194No Hit
CGGGCTGAGACTTGAAGCGGGT5370.2833145160439375No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGC5280.2785662280655475No Hit
TATTAATGCTGTAGGTGAAAGACA5260.2775110529592386No Hit
GAGGTCCGGATTTGGGGAGGGTTATCTACT5110.2695972396619219No Hit
TGAAAATCTTTGCTCTGAAGTGGA5080.26801447700245856No Hit
TCAGGTCTAGGCTGGTTAGTTT5070.2674868894493041No Hit
GTGGAGCCGGCCTGGGCTG4880.25746272593936964No Hit
CACCCCTCCTCCAAATACTTGACCTCTT4840.25535237572675185No Hit
TGTTTGCACTGACTTCTGAAAATTGGAAGC3690.19467980711399055No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC3610.190459106688755No Hit
CTCGGGCTGAGACTTGAAGCG3590.18940393158244612No Hit
TCTCGGGCTGAGACTTGAAGCGG3480.18360046849774722No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA3440.18149011828512943No Hit
CAATGTTTCGGCTTGTTCCCG3350.1767418303067394No Hit
CTGGCAACCTACCGCTAGTGGATGTGT3330.1756866552004305No Hit
TTAGACGTTAGAAATTCTTGCCTCACACA3290.17357630498781274No Hit
TAGAACTTGCCGATTTGTTATAAAGAC3230.17041077966888607No Hit
TAACGGTTTGTTTCTTCCACAATG3000.15827626594633382No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCT2910.1535279779679438No Hit
TTCGTGTGGCTTCTCTGACTTAGACATC2910.1535279779679438No Hit
GTGGAGCCGGCCTGGGCT2900.15300039041478933No Hit
AGCTGTCGACTCCTGCACCAAGAAGAGTGT2830.14930727754270823No Hit
TTGTAGTTGATTAAGTGGTAATAGCCAGGC2830.14930727754270823No Hit
CTCGGGCTGAGACTTGAAGCGGGC2760.1456141646706271No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGCG2690.14192105179854597No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGC2590.1366451762670015No Hit
TCCGATTTGCGATGGCTTTAGAGCGAAGT2420.12767618786337592No Hit
TTCATCTGTTTTACTAAAATCTGCAAAGC2350.12398307499129481No Hit
ACAGCCCGTTCAAGATAGTCGAACTCGACT2340.12345548743814036No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTA2320.12240031233183148No Hit
CACTAACTTGACCTCATT2310.12187272477867703No Hit
CTCGGGCTGAGACTTGAAGCGGGTG2300.12134513722552256No Hit
TCTCAGCTCACCAATCTCTGCT2280.1202899621192137No Hit
ACGATTTGGAACTTTTCGATGATCAGCGCC2210.11659684924713255No Hit
TGAAAATCTTTGCTCTGAGGTGGAA2130.11237614882189699No Hit
TGCCTAAAATCGTCAGGAGGTTGAATAGC2120.11184856126874256No Hit
TGAAAATCTTTGCTCTGAAGTGGGA2040.10762786084350699No Hit
TCATACAATGGACAAGGATCAGG1980.1044623355245803No Hit
TCTCGGGCTGAGACTTGAAGCGGGC1930.10182439775880807No Hit
CCCCCCTCCCCGCTCTTAGACCTCACT1930.10182439775880807No Hit
TTCAACGTGACAACCTGTATGGCTTCCATT1930.10182439775880807No Hit
CCCCCCTCCCCGCTCTTAGACCTCAC1930.10182439775880807No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)