FastQCFastQC Report
Wed 30 Apr 2025
1E7-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1E7-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82475
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA1340916.258260078811762No Hit
TGAAAATCTTTGCTCTGAAGTGGTA5480.6644437708396483No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAA4840.5868444983328281No Hit
TATTAATGCTGTAGGTGAAAGACA4760.5771445892694755No Hit
TGAAAATCTTTGCTCTGAAGTGGGAA4450.5395574416489846No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT3820.46317065777508337No Hit
TGAAAATCTTTGCTCTGAAGTGGA3810.4619581691421643No Hit
GATCGGCTCATTAATTCATGGA3380.4098211579266445No Hit
GTGTGCTCAGGATCTGTGACGTTTACTTT2970.3601091239769627No Hit
CACCCCTCTTCCACTAACTTGACCTCAT2700.3273719308881479No Hit
ATGTACAATTCTAGACTCTTTGGTGGT2620.3176720218247954No Hit
TCCCGTAGATCCGAACTTGT2140.2594725674446802No Hit
CACCCCTCTTCCACTAACTTGACCTCATT2050.2485601697484086No Hit
GTTCTTCTTCGCAAAGACTGTGTGTGCT2000.24249772658381327No Hit
TGAAAATCTTTGCTCTGAAGTGAA1770.21461048802667473No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACG1750.2121855107608366No Hit
GTATTGTAAGTGCTGAGAGT1750.2121855107608366No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA1660.20127311306456502No Hit
AACTTTTGACGGTGGATCTCTTGGCTCACG1650.20006062443164596No Hit
TCATACAATGGACAAGGATCAGG1640.1988481357987269No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC1610.19521066989996969No Hit
TTGGAAGGGATTTGAATT1570.19036071536829344No Hit
CTGGCAACCTACCGCTAGTGGATGTGT1560.18914822673537438No Hit
TTGTTTATGCCGTGAGGATTGGAGGAAAA1510.18308578357077904No Hit
GCGTGTTGTCTTCGACCTATTCTCAAACTT1310.1588360109123977No Hit
AATTGTGAGGTGTGTCTTTTCTTAAAGC1300.15762352227947862No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTT1290.15641103364655956No Hit
GAGGTCCGGACGGAGGAGGGTTATCCAC1260.15277356774780237No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAA1250.1515610791148833No Hit
GTGTGCTCAGGATCTGTGACGTTTA1240.15034859048196422No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGC1170.14186117005153076No Hit
GTGTGCTCAGGATCTGTGACGTT1160.1406486814186117No Hit
ATTGTGAGGTGTGTCTTTTCTTAAAGC1130.13701121551985448No Hit
GAGGTCCGGACGGAGGAGGGTTAT1080.13094877235525917No Hit
GAGGTCCGGATTTGGGGAGGGTTATCTACT1040.1260988178235829No Hit
GAGGTCCGGACGGTTGAGGGTTATC1020.12367384055774477No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCT1010.12246135192482571No Hit
TAGTGATAGCGTCTTGGCTCAAAAAA1010.12246135192482571No Hit
TATTAATGCTGTAGGTGAAAGACCA1000.12124886329190664No Hit
TAATTGTGAGGTGTGTCTTTTCTTAAAGC1000.12124886329190664No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCT970.11761139739314944No Hit
TATAGACTTGGAAGCGGGAAAAAAAAAAA950.1151864201273113No Hit
AGTGTGCTCAGGATCTGTGACGTTTACTTT940.11397393149439224No Hit
TATAGACTTGGAAGCGGGAAAAAAAAAAAA920.1115489542285541No Hit
AACTTTTGACGGTGGATCTCTTGGCTCACGT910.11033646559563504No Hit
TATAGACTTGGAAGCGGGAAAAAAAAAA880.10669899969687784No Hit
TGGGAATACCCCGTGTTGTAGGCTT880.10669899969687784No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTA830.10063655653228251No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)