FastQCFastQC Report
Wed 30 Apr 2025
1D7-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1D7-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7611859
Total Bases201 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA188819024.805898270054662No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGC1950302.562186188682686No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC1591452.090750761410583No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA804031.0562859874309285No Hit
TGAAAATCTTTGCTCTGAAGTGGA454150.5966348036662266No Hit
TATTAATGCTGTAGGTGAAAGACA327690.4304993037837406No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAA242360.31839791041846677No Hit
TGAAAATCTTTGCTCTGAAGTGG215610.28325537821969643No Hit
TTGTTTATGCCGTGAGGATTGGAGGAAAA206370.2711164250415043No Hit
GAGGTCCGGACGGAGGAGGGTTATCCAC194700.2557850848261903No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC186860.24548536697802734No Hit
CTCGGGCTGAGACTTGAAGCGG184130.24189885808447056No Hit
CTCGGGCTGAGACTTGAAGCGGGT170000.22333571864639112No Hit
TAGTGATAGCGTCTTGGCTCAAAAAA168220.22099726229821126No Hit
TCATACAATGGACAAGGATCAGG163470.21475699957132682No Hit
TCAGGTCTAGGCTGGTTAGTTT159860.2100143998988946No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT156990.20624396747233492No Hit
TCCCGTAGATCCGAACTTGT139420.1831615640804697No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC139360.18308273970918273No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTT137100.18011368839070718No Hit
TGAAAATCTTTGCTCTGAAGTGGTA128100.16829003269766296No Hit
TGAAAATCTTTGCTCTGAAGTGGGA123310.1619972203899205No Hit
TCCCGTAGATCCGAACTTGTGG120250.15797717745428547No Hit
TCCGATGTCTGGTACCTTTGTGGTCTTGG119170.15655833877112016No Hit
TATTAATGCTGTAGGTGAAAGACC116270.15274849415891703No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAA111630.146652742779392No Hit
GTGGAGCCGGCCTGGGCTG106960.14051757921422348No Hit
TTGTAGTTGATTAAGTGGTAATAGCCAGGC103640.1361559640030116No Hit
TTTACTAAAGGTTGCCGTGAATGGGA102460.1346057513677014No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT101600.13347593537925492No Hit
TATTAATGCTGTAGGTGAAAGACCA94750.1244768196573268No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGT93390.12269013390815567No Hit
CAATGTTTCGGCTTGTTCCC86640.1138223921383725No Hit
TGAAAATCTTTGCTCTGAAGTGGAG86020.11300787363507389No Hit
TGGCTGTTTGGTCACATACACTTTGAAGT85750.11265316396428257No Hit
TCTCGGGCTGAGACTTGAAGCGGGT85540.11237727866477822No Hit
TGAAAATCCTTGCTCTGAAGTGGAA84460.11095843998161291No Hit
TATTAATGCTGTAGGTGAAAGACCAA81380.10691212225554887No Hit
CTCGGGCTGAGACTTGAAGCG78980.10375914740407041No Hit
AGCTGTCGACTCCTGCACCAAGAAGAGTGT77630.10198559905011378No Hit
CACCCCTCTTCCACTAACTTGACCTCATT76320.10026460027701511No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)