FastQCFastQC Report
Wed 30 Apr 2025
1D5-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1D5-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11415885
Total Bases294.5 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA5754895.041124713502282No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC1621491.420380461085584No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT656400.5749882729197079No Hit
CTCGGGCTGAGACTTGAAGCG390920.34243512439026846No Hit
CACTGCCAATACCAAACTCTAATGTTGCT388950.3407094587936021No Hit
CCGAGCTGTTCTTCTTCGCAAAGACT296670.2598747271893506No Hit
CTCGGGCTGAGACTTGAAGCGGGT263230.23058221066522655No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTT251700.2204822490766156No Hit
TCAGGTCTAGGCTGGTTAGTTT233970.204951258706618No Hit
CCGAGCTGTTCTTCTTCGCAAAGACTGT225270.19733029896499485No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC215900.18912243772602824No Hit
TGATTAGATCTAATGGTTTTTGAGCAAATT198900.17423090719641973No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA191050.16735452398127698No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC175560.15378571175165132No Hit
TGGGAATACCCCGTGTTGTAGGCTT165810.14524498100672878No Hit
TGAAAATCTTTGCTCTGAAGCGGAA164750.14431645027958848No Hit
GAGGTCCGGACGGAGGAGGGTTATCCAC163560.14327404314251588No Hit
TCTCGGGCTGAGACTTGAAGCGGGT156440.1370371197677622No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC148580.1301519768287785No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT144540.12661304839703624No Hit
CCGAGCTGTTCTTCTTCGCAAAGACTGTGT141980.12437055909375401No Hit
GCCGAGCTGTTCTTCTTCGCAAAGACTGT140530.12310039913681682No Hit
AACTTTTGACGGTGGATCTCTTGGCTCACGT136240.1193424776090509No Hit
ACCCCGTGTTGTAGGCTT135150.11838766771038776No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA133670.11709122858192772No Hit
TGAAAATCTTTGCTCTGAAGTGGA131610.11528672547069282No Hit
CTCGGGCTGAGACTTGAAGCGG128290.11237849715549866No Hit
CCGAGCTGTTCTTCTTCGCAAAGACTG127150.1113798886376308No Hit
GCCGAGCTGTTCTTCTTCGCAAAGACT124550.1091023604389848No Hit
TACCCCGTGTTGTAGGCTT117720.10311946905561856No Hit
GGGACCGTAGTTCAACTGGTTAGAGCACC114530.10032511715035672No Hit
CTCGGGCTGAGACTTGAAGCGGGTG114280.10010612405433306No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)