FastQCFastQC Report
Wed 30 Apr 2025
1D2-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1D2-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32445344
Total Bases914.6 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC11745723.6201557918448946No Hit
TGAAAATCTTTGCTCTGAAGTGGAA10638513.278901897295341No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT8404992.5905072851130813No Hit
TCAGGTCTAGGCTGGTTAGTTT3127380.9638917682611101No Hit
TGACCACTTTGGATTCTTGCGCCATTCTT2739270.8442721396327313No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC2176870.6709344798440109No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT1987920.6126980808093759No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT1566610.48284585917782225No Hit
TCCCGTAGATCCGAACTTGT1442290.44452911332978934No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA1225890.3778323324295776No Hit
GAGGTCCGGACGGAGGAGGGTTATCCAC860140.2651042935467104No Hit
CACCCCTCTTCCACTAACTTGACCTCAT855360.2636310467227594No Hit
TCAAGTCTAGGCTGGTTAGTTT802250.24726198002400593No Hit
GAGGTCCGGATTTGGGGAGGGTTATCTACT787570.24273744793705995No Hit
AGCTGTCGACTCCTGCACCAAGAAGAGTGT750120.23119495974522572No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTT702110.21639776727286356No Hit
TTCAACGTGACAACCTGTATGGCTTCCATT601800.18548115871417484No Hit
GGTGAAACACGTTTTGGTCATGTATGATT599320.18471679634526297No Hit
AAAGAAGTACAAGTGGTAGGG566670.1746537191900323No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC492770.15187695343898958No Hit
TCAGGTCTAGGCTGGTTAGTT490100.15105403104987886No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACG487270.15018179495954798No Hit
TCAGGTCTAGGCTGGTTAGTTC486400.14991365170916357No Hit
TCTCAGCTCACCAATCTCTGCT476250.14678531378801224No Hit
TGCTTGACCTTTTTCTGCCATTCTTTGAC469240.14462475725330576No Hit
CACCCCTCTTCCACTAACTTGACCTCATT464550.14317924938629098No Hit
TTAGACGTTAGAAATTCTTGCCTCACACA456320.1406426758797811No Hit
ACACGATCAATTGTCCGAAGCAACTGCAAT440510.135769865777968No Hit
TGGAAACGTCGTTGGTCTAAGTAGTGTT438750.13522741506454672No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGCG400270.12336746992110793No Hit
TGACCACTTTGGATTCTTGCGCCATTCTC397080.12238427800303181No Hit
TCCTCCGGTTTGTCTTTGTCCTTGACTT376780.11612760216072913No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACC376670.1160936989911403No Hit
CACCCCTCCTCCAAATACTTGACCTCTT363990.11218558816944582No Hit
TCCATGATCCACGATCTTCTACACTTTTTT353020.10880451752954137No Hit
TAACGGTTTGTTTCTTCCACAATG339980.10478545088010163No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)