FastQCFastQC Report
Wed 30 Apr 2025
1D12-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1D12-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118997
Total Bases2.9 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA3634430.54194643562443No Hit
TTGGAAGGGATTTGAATT69205.8152726539324515No Hit
TGAAAATCTTTGCTCTGAAGCGGAA16341.3731438607695994No Hit
TGAAAATCTTTGCTCTGAAGTGGA11150.9369984117246652No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAA6640.557997260435137No Hit
TGAAAATCTTTGCTCTGAAGTGG4790.4025311562476365No Hit
TTTGGAAGGGATTTGAATT4620.3882450818087851No Hit
TGAAAACCTTTGCTCTGAAGTGGAA4040.33950435725270384No Hit
TCCGATGTCTGGTACCTTTGTGGTCTTGG3760.31597435229459564No Hit
TCCCGTAGATCCGAACTTGT3370.2832004168172307No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA3330.27983898753750097No Hit
TCATACAATGGACAAGGATCAGG3070.25798969721925763No Hit
TATTAATGCTGTAGGTGAAAGACA2920.2453843374202711No Hit
CAATGTTTCGGCTTGTTCCC2810.23614040690101432No Hit
TTGTTTATGCCGTGAGGATTGGAGGAAAA2680.22521576174189267No Hit
CACCCCTCTTCCACTAACTTGACCTCAT2610.21933326050236562No Hit
GAGGTCCGGACGGAGGAGGGTTATCCAC2500.21008932998310884No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT2350.1974839701841223No Hit
GTTCTTCCTCGCCGAGACCGAGTGTGCT2280.19160146894459523No Hit
TAGTGATAGCGTCTTGGCTCAAAAAA2260.18992075430473038No Hit
TCAGTATCTTCGTCGCCAGAGCAGAGA2150.18067682378547356No Hit
CAATGTTTCGGCTTGTTCCCA2100.1764750371858114No Hit
TGAAAATCTTTGCTCTGAAGTGGTA2060.17311360790608166No Hit
CACCCCTCTTCCACTAACTTGACCTCATT2050.17227325058614923No Hit
CTTGATGAAGAAATGCTATGTAAGCTTCACC2010.1689118213064195No Hit
AGCACTGATGACTGTTCAGTTTTTCTGAATT1950.16386967738682487No Hit
TATTAATGCTGTAGGTGAAAGACCAA1940.16302932006689244No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC1870.1571468188273654No Hit
TGAAAATCTTTGCTCTGAAGT1800.15126431758783834No Hit
TGAAAATCTTTGCTCTGAAGTGGGA1720.14454145902837887No Hit
TGAAAATCTTTGCTCTGAAGCGGA1660.13949931510878424No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAA1650.13865895778885182No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGC1580.13277645654932477No Hit
TCAGGTCTAGGCTGGTTAGTTT1550.13025538458952748No Hit
CGATCAAGATAGTCGAACTCGACCCTGTC1290.10840609427128416No Hit
TGAAAATCTTTGCTCTGAGGTGGAA1290.10840609427128416No Hit
CACCCCTCCTCCAAATACTTGACCTCTT1260.10588502231148685No Hit
TATAGACTTGGAAGCGGGAAAAAAAAAAA1250.10504466499155442No Hit
TGAAAATCCTTGCTCTGAAGTGGAA1250.10504466499155442No Hit
TAAAAAACTCGTCGACGTG1210.10168323571182467No Hit
TGAAAATCTTTGCCCTGAAGTGGAA1210.10168323571182467No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)