FastQCFastQC Report
Wed 30 Apr 2025
1D1-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1D1-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10330343
Total Bases250.1 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA1329601.2870821423838492No Hit
CGAAAGACGAACTACTGCGAAAGCATT1252101.2120604320689061No Hit
AGTGTGCTCAGGATCTGTGACGTTTACTTT748360.724428995242462No Hit
AGTGTGCTCAGGATCTGTGACGTTTACT624060.604103852117979No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCT555270.537513614020367No Hit
AGTGTGCTCAGGATCTGTGACGTTTAC488880.4732466288873467No Hit
CGAAAGACGAACTACTGCGAAAGCAT435800.42186401748712504No Hit
AGTGTGCTCAGGATCTGTGACGTT389950.37748020564273616No Hit
GTGTGCTCAGGATCTGTGACGTTTACT368190.3564160454304373No Hit
AGTGTGCTCAGGATCTGTGA337190.3264073613044601No Hit
AAGACGAACTACTGCGAAAGCATT329810.31926335843834036No Hit
AGATGTCCGACTTGCTTAATTGAAGCC325130.31473301515738633No Hit
AGTGTGCTCAGGATCTGTGAC310180.3002610852321167No Hit
ATTGAATCTTTGAACGCAAATGGCGCTCTT293840.28444360463152096No Hit
AAAGACGAACTACTGCGAAAGCATT275500.26669007989376536No Hit
GATGTCCGACTTGCTTAATTGAAGCC269130.26052377931691134No Hit
TTGAATCTTTGAACGCAAATGGCGCTCTT268520.25993328585507763No Hit
AAGATGTCCGACTTGCTTAATTGAAGCC257080.2488591133905234No Hit
GTGTGCTCAGGATCTGTGACGTTTACTTT254850.24670042417759022No Hit
CTTAACTGAGTGTGCTCAGGATCTGTGA241960.23422261971359518No Hit
CTGCGAACTTCGGTGAAAGCTGA241100.23339012073461649No Hit
AGTGTGCTCAGGATCTGTGACGTTTA229300.2219674603253735No Hit
GTGTGCTCAGGATCTGTGACGTTTAC228610.2212995250980534No Hit
AGTGTGCTCAGGATCTGTGACGT219080.21207427478448682No Hit
AGGATCTGTGACGTTTAC212730.2059273346490044No Hit
GTGTGCTCAGGATCTGTGACGTT209510.2028103035881771No Hit
AAGACGAACTACTGCGAAAGCAT204380.19784435037636214No Hit
AGTGTGCTCAGGATCTGTGACG200880.19445627313633246No Hit
GATGTCCGACTTGCTTAATTGAAGC193880.18768011865627307No Hit
TCTTAACTGAGTGTGCTC191290.18517294149865113No Hit
TGAGTGTGCTCAGGATCTGTGA190040.1839629139129262No Hit
AGATGTCCGACTTGCTTAATTGAAGC185450.17951969261814443No Hit
GTGTGCTCAGGATCTGTGA184780.1788711178321959No Hit
CATTGAATCTTTGAACGCAAATGGCGCTCTT182460.1766253066330905No Hit
GTGTGCTCAGGATCTGTGACGT175050.16945226310491338No Hit
TGAGTGTGCTCAGGATCTGTGACGTTTAC167350.16199849317684803No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCT162250.15706158062709052No Hit
TGAGTGTGCTCAGGATCTGTGACGT156480.15147609329138442No Hit
TGAATCTTTGAACGCAAATGGCGCTCTT156430.15142769218795543No Hit
GTGTGCTCAGGATCTGTGAC156230.15123408777423944No Hit
GAAAGACGAACTACTGCGAAAGCATT156100.15110824490532404No Hit
TCTTCTTCGCAAAGACTGTGTGTG154510.149569089816282No Hit
GTGTGCTCAGGATCTGTGACGTTTA152580.1477008072239228No Hit
ACGAACTACTGCGAAAGCATT145840.14117633848169417No Hit
TGCTACGATCTTCTGAGAT137300.13290943001602173No Hit
TCTTCTTCGCAAAGACTGTGTGTGCT135850.13150579801658085No Hit
ACGAACTACTGCGAAAGCAT132830.12858237136946954No Hit
GCGGTTGTAGCTCAGTTGGTTAGAGTA132200.1279725174662642No Hit
TGCTACGATCTTCTGAGA130330.12616231619801976No Hit
CGCGGGGTAGAGCAGTCTGGTAGCTCGTC129780.1256299040603008No Hit
TCCGGCGTAGCTCAGCGGTAGAGCA127640.1235583368335398No Hit
GCTGTTCTTCTTCGCAAAGACTGTGTGTGCT126470.12242575101330129No Hit
CCTTTGTTCTAAGATTTGT126240.12220310593752792No Hit
AAAGACGAACTACTGCGAAAGCAT123600.11964752767647695No Hit
TGAGTGTGCTCAGGATCTGTGAC122190.11828261655977927No Hit
AGACTGTGTGTGCTCTTAACTGAGTGTGCTC121660.11776956486343193No Hit
AAGATGTCCGACTTGCTTAATTGAAGC116730.11299721606533297No Hit
AGTGTGCTCAGGATCTGTGACGTTTACTTTG111460.10789573976391685No Hit
TCTTCTTCGCAAAGACTGTGTGTGC110880.1073342869641405No Hit
GTACCGAAGCTGTGGACTTGT110340.10681155504710733No Hit
GTGTCCGGTGCGTTCTCGACG106510.10310403052444629No Hit
GCGAACTTCGGTGAAAGCTGA104330.10099374241494206No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)