FastQCFastQC Report
Wed 30 Apr 2025
1C6-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1C6-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20668
Total Bases480.2 kbp
Sequences flagged as poor quality0
Sequence length15-31
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA7243.5029998064640995No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC1860.8999419392297271No Hit
GCTTAGGGTACTGTCGGCT1560.7547900135475131No Hit
CATAGTAACTGATCGAAT720.34836462163731374No Hit
GTGTGCTCAGGATCTGTGAC580.2806270563189472No Hit
GTATTGTAAGTGCTGAGAGT560.2709502612734662No Hit
GAGTGTGCTCAGGATCTGTGAC560.2709502612734662No Hit
ATGCACATTGCTAGAACCTGGCAGA530.25643506870524485No Hit
TGTTTTACGGTGCGAAAACGATGTTCA490.23708147861428294No Hit
ATAAAGAACGGGGTATTGTAAGTTGTAGAGT470.227404683568802No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGC470.227404683568802No Hit
GAGGTCCGGACGGAGGAGGGTTATCCAC470.227404683568802No Hit
GTGTGCTCAGGATCTGTGACGTTTACTTT440.2128894910005806No Hit
GGGGACTAGTTGGGAAGT440.2128894910005806No Hit
CATCTTGCTGAAGAACGTATCTGCA400.19353590090961872No Hit
GTGTGCTCAGGATCTGTGA400.19353590090961872No Hit
TGAAAATCTTTGCTCTGAAGTGGA390.18869750338687827No Hit
AGTGTGCTCAGGATCTGTGAC390.18869750338687827No Hit
GTGCTTAGGGTACTGTCGGCT380.18385910586413778No Hit
GTGTGCTCAGGATCTGTGACGTTTA340.1645055157731759No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCT330.15966711825043545No Hit
CATAGTAACTGATCGAATC320.154828720727695No Hit
ATGCACATTGCTAGAACCTGGCAGAAG320.154828720727695No Hit
GCTTGTGCGTGATCGATGGCCGTCTGAAGC320.154828720727695No Hit
GAGTGTGCTCAGGATCTGTGACGTTTACTTT310.14999032320495453No Hit
CCTCATTGTTGAACATTG310.14999032320495453No Hit
TCCCGTAGATCCGAACTTGT300.14515192568221405No Hit
ATTGTAAGTGCTGAGAGT290.1403135281594736No Hit
GTGTGCTCAGGATCTGTGACGT290.1403135281594736No Hit
TGAAAATCTTTGCTCTGAAGT290.1403135281594736No Hit
AAAGACACGTGACCAATCTGATCCTG280.1354751306367331No Hit
GAAAAGACAATGGTGCGAAGC280.1354751306367331No Hit
CATCTTCCTGAAGAACGTATCTGCA270.13063673311399263No Hit
GTGCTCAGCTTCTGGACGTTGC270.13063673311399263No Hit
AGCACTAGTACTGTGAAACTGCGAATG270.13063673311399263No Hit
AGAACGGGGTATTGTAAGTTGTAGAGT260.12579833559125217No Hit
ACAGATTGATAGCTCTTT250.12095993806851171No Hit
ATGTACAATTCTAGACTCTTTGGTGGT250.12095993806851171No Hit
GATTAAGCCTTTGTTCTAAGATTTGT250.12095993806851171No Hit
GAGGTCCGGACGGTTGAGGGTTATC240.11612154054577124No Hit
GCAAAGACTGTGTGTGCT240.11612154054577124No Hit
TGAAAAGACAATGGTGCGAAGC240.11612154054577124No Hit
CGCTTGTGCGTGATCGATGGCCGTCTGAAGC230.11128314302303076No Hit
GTGGCCCTATCGAAGCTCAAAGC230.11128314302303076No Hit
GTGTCTTCACGAGCTTGCTCTGGA220.1064447455002903No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT220.1064447455002903No Hit
TGTTTTACGGTGCGAAAACGATGTTC220.1064447455002903No Hit
GAGTGTGCTCAGGATCTGTGACGTTTAC210.10160634797754985No Hit
CGAGTTAGATAGAATAAGGTC210.10160634797754985No Hit
AGTGTGCTCAGGATCTGTGACGTTTACTTT210.10160634797754985No Hit
CTTAGGGTACTGTCGGCT210.10160634797754985No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)