FastQCFastQC Report
Wed 30 Apr 2025
1C5-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1C5-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28120629
Total Bases776.7 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA17639606.27283265961085No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC9010293.204156635329886No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT6640022.361262971749316No Hit
TCAGGTCTAGGCTGGTTAGTTT4434001.5767783857181856No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT3322701.1815880789864266No Hit
TGACCACTTTGGATTCTTGCGCCATTCTT2311520.8220015277752145No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1841000.6546795237048219No Hit
TCCCGTAGATCCGAACTTGT1646920.585662575328596No Hit
GAGGTCCGGATTTGGGGAGGGTTATCTACT1577990.5611503213530536No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT1386920.49320376155170637No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA1152480.40983436039072957No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTA1083940.3854607946358526No Hit
CACCCCTCTTCCACTAACTTGACCTCAT1016190.36136816143052847No Hit
TCAAGTCTAGGCTGGTTAGTTT999300.35536189464325285No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC970550.3451380835044622No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGCG834520.296764343358038No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGC815950.2901606503894347No Hit
AGCTGTCGACTCCTGCACCAAGAAGAGTGT769550.27366030823848214No Hit
TCAGGTCTAGGCTGGTTAGTTC746720.2655417131672268No Hit
TCAGGTCTAGGCTGGTTAGTT688190.2447278117427601No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACC660750.23496985078107605No Hit
TTAGACGTTAGAAATTCTTGCCTCACACA630970.2243797604954No Hit
TCATATCGCTCAATTCATCTGAAACATTTC609740.21683014273969478No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTT599980.2133593811148392No Hit
GAGGTCCGGACGGAGGAGGGTTATCCAC557840.19837394106653874No Hit
CACCCCTCTTCCACTAACTTGACCTCATT544600.19366565378036174No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTA503900.17919229331605635No Hit
TTCAACGTGACAACCTGTATGGCTTCCATT430870.15322203496941694No Hit
TGAAAATCTTTGCTCTGAAGTGGA425950.1514724297241004No Hit
GAGGTCCGGATTTGGGGAGGGTTATCTACTA420020.14936365754834288No Hit
CCCCCCTCCCCGCTCTTAGACCTCACT414300.14732956364525132No Hit
CTCGGGCTGAGACTTGAAGCGG408580.14529546974215976No Hit
TCCAGTCAACTTTGTATCTGTCCCAGAGT403280.14341073238440008No Hit
CCCCCCTCCCCGCTCTTAGACCTCAC396060.1408432222479803No Hit
TCAGATCAACTGTTTGCTCCAAAAACATT395480.1406369679710934No Hit
TCCCCCCTCCCCGCTCTTAGACCTCAC395350.14059073856420495No Hit
TAACGGTTTGTTTCTTCCACAATG388850.13827926821978273No Hit
TTTGCTCTTTCAACACTTCTAACTTTGT380840.13543082553381008No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGT363080.1291151773312041No Hit
TCCCGTAGATCCGAACTTGC362710.1289836013269831No Hit
TCTCAGCTCACCAATCTCTGCT350050.12448156831769304No Hit
TGACCACTTTGGATTCTTGCGCCATTCTC340780.1211850559957247No Hit
TCTCCTTCTGATGCTTTGTTTATT329400.11713820483887469No Hit
AAAGAAGTACAAGTGGTAGGG329040.11701018494287593No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACG317300.11283531389002714No Hit
GAGGTCCGGATTTGGGGAGGGTTATCTACC316100.11240858090336457No Hit
TGAAAATCTTTGCTCTGAAGTGGAT310860.11054518019493803No Hit
AGCTGTCGACTCCTGCACCAAGAAGAGTGTT305670.10869956002762242No Hit
CACCCCTCCTCCAAATACTTGACCTCTT304660.10834039309718144No Hit
TACACGATCAGAGTGAACACAACTAGCT287790.10234123852635017No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGCA287740.10232345798523923No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)