FastQCFastQC Report
Wed 30 Apr 2025
1C12-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1C12-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1421752
Total Bases38.6 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA578764.07075214242709No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC548163.8555247328648035No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT396432.7883203259077534No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGC123900.8714600014629836No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC116620.8202555719984919No Hit
TCAGGTCTAGGCTGGTTAGTTT99990.7032872118344128No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT85310.6000343238483223No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT79230.5572701849549007No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGT55920.3933175406118648No Hit
CTCGGGCTGAGACTTGAAGCGG49350.34710694973525624No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC42250.29716856385642504No Hit
TGACCACTTTGGATTCTTGCGCCATTCTT41960.2951288269684164No Hit
CTCGGGCTGAGACTTGAAGCGGGT35980.25306804562258395No Hit
TCCCGTAGATCCGAACTTGT35940.2527867026035483No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA32220.22662180183323113No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC31930.2245820649452225No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTT28750.20221529493188684No Hit
TGCTGTCTATTCCTGCACCTGAAAGAGATT28340.19933152898677126No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGT25520.1794968461447566No Hit
TGTGGCTATCCCAGACTGATAAATTGATATT25220.17738677350198911No Hit
TCCGCTGTAGTATAGTGGTTAGTATTTCC23760.16711775330718717No Hit
TCAAGTCTAGGCTGGTTAGTTT23280.16374163707875916No Hit
TCCGCTGTAGTATAGTGGTTAGTATTTC22740.15994350632177765No Hit
TGAAAATCTTTGCTCTGAAGTGGAG22110.15551235377196584No Hit
TCTCGGGCTGAGACTTGAAGCGGGT21800.15333194537443942No Hit
TCTCAGCTCACCAATCTCTGCT20900.14700172744613688No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT20300.14278158216060186No Hit
GAGGTCCGGATTTGGGGAGGGTTATCTACT18660.13124651838013943No Hit
TTCAACGTGACAACCTGTATGGCTTCCATT17540.12336891384714072No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCG16370.1151396305403474No Hit
TGGAAACGTCGTTGGTCTAAGTAGTGTT16190.11387358695468688No Hit
TGAAAATCTTTGCTCTGAAGTGGA15410.10838739808349135No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)