FastQCFastQC Report
Wed 30 Apr 2025
1B7-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1B7-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80353
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA2468830.724428459422796No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCT28283.519470337137381No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCT14311.7808918148668997No Hit
TGAAAATCTTTGCTCTGAAGTGGA7470.9296479285154258No Hit
TTACCGTGACCGATCCCGT6460.8039525593319478No Hit
TCCCGTAGATCCGAACTTGT3060.38081963336776475No Hit
TGAAAATCTTGGCTCTGAAGTGGAA2990.3721080731273257No Hit
TGAAAATCTTTGCTCTGAAGTGGTA2810.34970691822333955No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA2740.3409953579829005No Hit
TGAAAATCTTTGCTCTGAAGTGG2710.33726183216556943No Hit
TGAAAATCTTTGCTCTGAAGT2680.3335283063482384No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC2670.33228379774246136No Hit
TATTAATGCTGTAGGTGAAAGACA2340.2912150137518201No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAA2260.281258944905604No Hit
TCCGATCTGTTTTCAAGGTTTTGACCAC2210.27503640187671896No Hit
TAAAAAACTCGTCGACGTG2130.26508033303050293No Hit
TGAAAATCTTTTCTCTGAAGTGGAA2110.2625913158189489No Hit
CACCCCTCTTCCACTAACTTGACCTCAT2070.25761328139584083No Hit
CACCCCTCTTCCACTAACTTGACCTCATT2050.25512426418428685No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGC1960.24392368673229375No Hit
TGTTTTACGGTGCGAAAACGATGTTCA1840.22898958346296966No Hit
TCAGGTCTAGGCTGGTTAGTTT1710.21281097158786852No Hit
TTGTTTATGCCGTGAGGATTGGAGGAAAA1620.20161039413587545No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACG1560.1941433425012134No Hit
GAGGTCCGGACGGAGGAGGGTTATCCAC1500.18667629086655133No Hit
TGAGAATCTTTGCTCTGAAGTGGAA1400.17423120480878126No Hit
TCATACAATGGACAAGGATCAGG1380.17174218759722723No Hit
TGAAAATCTTTGCTCTGAAGTGAA1340.1667641531741192No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT1330.16551964456834217No Hit
TAGTGATAGCGTCTTGGCTCAAAAAA1320.16427513596256518No Hit
TTAGACGTTAGAAATTCTTGCCTCACACA1180.14685201548168705No Hit
TCCGATCTGTTTTCAAGGTTTTGACCACA1160.14436299827013305No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCC1120.139384963847025No Hit
TGAAAATCTTTGCTCTGAAGTGGGA1100.136895946635471No Hit
TGAAAATCCTTGCTCTGAAGTGGAA960.11947282615459286No Hit
TAAAAAACTCGTCGACGTGA960.11947282615459286No Hit
TGAAAATCTTTGCTCTGAGGTGGAA950.11822831754881584No Hit
TATTAATGCTGTAGGTGAAAGACCAA900.11200577451993081No Hit
TGAAAATCTTTGCCCTGAAGTGGAA900.11200577451993081No Hit
AAATGTTTCGGCTTGTTCCC880.10951675730837679No Hit
TAACGGTTTGTTTCTTCCACAATG880.10951675730837679No Hit
TGAAAATCTTTGCTCTGGAGTGGAA850.10578323149104576No Hit
CAATGTTTCGGCTTGTTCCC830.10329421427949174No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)