FastQCFastQC Report
Wed 30 Apr 2025
1B6-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1B6-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1334606
Total Bases34.4 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA465663.4891196353080987No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC440543.300899291626143No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT94680.7094228558840587No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT90420.6775033230781219No Hit
ATGTACAATTCTAGACTCTTTGGTGGT68970.5167817318369616No Hit
CTGGCAACCTACCGCTAGTGGATGTGT61670.4620839408784315No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTT55450.4154784258425333No Hit
TTGGAAGGGATTTGAATT46560.3488670064423508No Hit
GAGGTCCGGACGGAGGAGGGTTATCCAC44760.33537987990463103No Hit
GGTTAGGGTACTGTCGGCT42560.3188956141363069No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC42160.31589847490570255No Hit
GATCGGCTCATTAATTCATGGA35920.26914310290827403No Hit
GCGTGTTGTCTTCGACCTATTCTCAAACTT34790.26067618458181663No Hit
TGGGAATACCCCGTGTTGTAGGCTT29810.22336180116079202No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCT27470.20582853666175635No Hit
GCTTGTGCGTGATCGATGGCCGTCTGAAGCT24310.18215113673998168No Hit
GTGTCTTCACGAGCTTGCTCTGGA24000.17982835383626328No Hit
TCCCGTAGATCCGAACTTGT23200.17383407537505452No Hit
GAGGTCCGGACGGTTGAGGGTTATC22930.17181100639439656No Hit
GAGGTCCGGATTTGGGGAGGGTTATCTACT22700.17008765133679904No Hit
GATTGCTGTAGGCTGCTATGAGCCTTGGGC22630.16956315197144325No Hit
TGAAAATCTTTGCTCTGAAGTGGA22300.16709051210619463No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCT21000.1573498096067304No Hit
TGAAAATCTTTGCTCTGAAGCGGAA20290.1520298874724076No Hit
GTATTGTAAGTGCTGAGAGT20250.15173017354934715No Hit
ATTAGTGTTGATGATGGAATTTATGAAGGCT17830.1335974812041906No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC17680.13247355399271396No Hit
TGAAAATCTTTGCTCTGAAGT17330.1298510571659351No Hit
CTGTCAAGGTATTGGTTCAAGTCCATT17280.12947641476210955No Hit
CATCTTCCTGAAGAACGTATCTGCA16960.12707870337762606No Hit
GAGCAGTGAAGATATAGTAGTCTCGTT15660.11733800087816179No Hit
GAATTCTATCCATTGGTAGTGCATGGCT15380.11524000341673873No Hit
GTTAGGGTACTGTCGGCT14930.11186822178230879No Hit
AGCACTGATGACTGTTCAGTTTTTCTGAATT14530.1088710825517044No Hit
TTGGCAACCTTTAGCTAGTGGATGTGT14430.1081217977440533No Hit
AATTGTGAGGTGTGTCTTTTCTTAAAGC14160.10609872876339534No Hit
GTGTTGTCTTCGACCTATTCTCAAACTT14100.10564915787880469No Hit
TTTACTAAAGGTTGCCGTGAATGGGA13670.10242723320590497No Hit
GTGTGCTCAGGATCTGTGACGTTTACTTT13610.1019776623213143No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)