FastQCFastQC Report
Wed 30 Apr 2025
1B4-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1B4-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12038450
Total Bases316 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA4380843.639039909622917No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC1463251.2154803982240239No Hit
TTACCGTGACCGATCCCGT879000.7301604442432372No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT567200.47115700110894676No Hit
TTCTGTGGCGTGAGTGAGT520150.43207389655645034No Hit
CCAACTCCGACAGCATAGGTGTGCGATG409450.3401185368548277No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGC404340.3358738043518892No Hit
CTCGGGCTGAGACTTGAAGCGGGT399030.3314629375044129No Hit
CCAACTCCGACAGCATAGGTGTGCGATGT383830.31883672731954693No Hit
AATCCGCGAATCCGGGGTGCTGTAGTGC357100.29663287217208195No Hit
ATGTCGGTTCATCACTGAGGAGAGCGGCT330450.274495470762432No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA318600.2646520108485727No Hit
TGAAAATCTTGGCTCTGAAGTGGAA289200.2402302622015293No Hit
CTCGGGCTGAGACTTGAAGCGG288880.23996444725026891No Hit
GTTACCGTGACCGATCCCGT272520.22637465786708422No Hit
CGAGTAGCGATTAGTGAATT257860.21419701041247005No Hit
TCAGGTCTAGGCTGGTTAGTTT198280.1647055891746861No Hit
CGAGTAGCGCTTAGTGAATT189650.1575368922078839No Hit
TTGTAGTTGATTAAGTGGTAATAGCCAGGC185310.15393177693141558No Hit
TCTCGGGCTGAGACTTGAAGCGGGT179600.14918864139486396No Hit
TCTGTGGCGTGAGTGAGT179410.14903081376755312No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTT177720.1476269785562095No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC175180.14551707238058056No Hit
GTGGAGCCGGCCTGGGCTG156570.1300582716213466No Hit
GTGTGCCGAAGAATCTCTGTTCTTTGAAGT154070.12798159231462522No Hit
TCCGCTGTAGTATAGTGGTTAGTATTTCCG153230.12728382806756683No Hit
CCAACTCCGACAGCATAGGTGTGCGAT152460.12664421084109667No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC151160.12556433760160152No Hit
AATCCGCGAATCCGGGGTGCTGTAGTGT147280.12234133131756995No Hit
ATCCGCGAATCCGGGGTGCTGTAGTGC141660.11767295623606028No Hit
TAATCCGCGAATCCGGGGTGCTGTAGTGC138690.1152058612196753No Hit
GTGCAGGCTCAAAAATCGGT138530.11507295374404511No Hit
CCCAGGAGCATGTCTGTCTGAGTGTCGGA130290.10822821874909144No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA128930.10709850520623503No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)