FastQCFastQC Report
Wed 30 Apr 2025
1B3-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1B3-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5254
Total Bases130.5 kbp
Sequences flagged as poor quality0
Sequence length15-31
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA112221.355157974876285No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCT3336.338028169014084No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCT2334.434716406547393No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGC500.9516558812333461No Hit
TGAAAATCTTTGCTCTGAAGTGGA430.8184240578606776No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGC420.7993909402360105No Hit
TATTAATGCTGTAGGTGAAAGACA290.5519604111153407No Hit
CATCTTCCTGAAGAACGTATCTGCA240.4567948229920061No Hit
TGAAAATCTTTTCTCTGAAGTGGAA190.3616292348686715No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAA170.32356299961933765No Hit
TGAAAATTTTTGCTCTGAAGTGGAA160.30452988199467074No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA140.26646364674533685No Hit
CCTTCCGTGATATAGCTGA130.24743052912066998No Hit
TAGTGATAGCGTCTTGGCTCAAAAAA120.22839741149600304No Hit
GTGTCTTCACGAGCTTGCTCTGGA110.20936429387133612No Hit
GCTTGCGAGCTGTTGCCA100.1903311762466692No Hit
TCATACAATGGACAAGGATCAGG100.1903311762466692No Hit
TGTGTGATGAGATCAGAGA100.1903311762466692No Hit
GTGTGCTTAAGCGTGAGTAGTT100.1903311762466692No Hit
GTGATAGATCTTTTGGAGACGA100.1903311762466692No Hit
CACCCCTCTTCCACTAACTTGACCTCAT100.1903311762466692No Hit
GTATTGTAAGTGCTGAGAGT90.17129805862200229No Hit
TGAAAATCTTTGCTCTGAAGT90.17129805862200229No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC90.17129805862200229No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT90.17129805862200229No Hit
TGAAAATCTTTGCTCTGAAGTGAA90.17129805862200229No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCTG90.17129805862200229No Hit
TAATTGTGAGGTGTGTCTTTTCTTAAAGC90.17129805862200229No Hit
TGAAAATCTTTGCTCTGAAGTGG80.15226494099733537No Hit
TCCCGTAGATCCGAACTTGT80.15226494099733537No Hit
AAGCGTGAGTAGTTGTCT80.15226494099733537No Hit
TATAGACTTGGAAGCGGGAAAAAAAAAAA80.15226494099733537No Hit
ATTGTGAGGTGTGTCTTTTCTTAAAGC80.15226494099733537No Hit
GAGGTCCGGAACTCAGGGGAGGGTTATC80.15226494099733537No Hit
GAGGTCCGGACGGAGGAGGGTTATCCAC80.15226494099733537No Hit
TATTAATGCTGTAGGTGAAAGACC70.13323182337266842No Hit
TCAGTATCTTCGTCGCCAGAGCAGAGA70.13323182337266842No Hit
TATCGACCGACTGTATAGCAAAT70.13323182337266842No Hit
AATGGTGTTTATCGACCGACTGTATAGC70.13323182337266842No Hit
TCCAAAACTGCATAGACGT70.13323182337266842No Hit
GTGCTCAGCTTCTGGACGTTGC70.13323182337266842No Hit
TAAAAAACTCGTCGACGTGA60.11419870574800152No Hit
TATTAATGCTGTAGGTGAAAGACCA60.11419870574800152No Hit
TATAGACTTGGAAGCGGGAAAAAAAAAAAA60.11419870574800152No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTA60.11419870574800152No Hit
CACCCCTCTTCCACTAACTTGACCTCA60.11419870574800152No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGCG60.11419870574800152No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGC60.11419870574800152No Hit
TGAGAATCTTTGCTCTGAAGTGGAA60.11419870574800152No Hit
CTGCTATGATGACTTAACTTA60.11419870574800152No Hit
TGAAAATCTTTGCCCTGAAGTGGAA60.11419870574800152No Hit
TCCGATGTCTGGTACCTTTGTGGTCTTGG60.11419870574800152No Hit
TAAAAAACTCGTCGACGTG60.11419870574800152No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)