FastQCFastQC Report
Wed 30 Apr 2025
1A7-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1A7-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8013931
Total Bases205.3 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA256864432.0522350392086No Hit
TGAAAATCTTTGCTCTGAAGTGGA579830.7235275671826973No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC550800.6873031474815543No Hit
TGAAAATCTTTGCTCTGAAGTGGAG479310.5980959905943787No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT394930.49280434283749136No Hit
CGAGTAGCGATTAGTGAATT394730.49255477742446246No Hit
TCAGGTCTAGGCTGGTTAGTTT375680.46878367183346104No Hit
TATTAATGCTGTAGGTGAAAGACA301710.3764819038247272No Hit
TTCTGTGGCGTGAGTGAGT239330.2986424515010174No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA232450.2900574012928237No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTT201760.25176158866354104No Hit
TGAAAATCTTTGCTCTGAAGTGGGA198790.24805554228006207No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTA196290.24493597461720096No Hit
AGCACTGATGACTGTTCAGTTTTTCTGAATT193950.24201605928476302No Hit
CAATGTTTCGGCTTGTTCCCG182310.2274913522464818No Hit
CTCGGGCTGAGACTTGAAGCGGGT182130.22726674337475578No Hit
CTCGGGCTGAGACTTGAAGCGG171290.21374029798859012No Hit
TCCCGTAGATCCGAACTTGT164420.20516772605104786No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGC162560.2028467677098792No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT153540.1915913675822764No Hit
TGAAAATCTTTGCTCTGAAGTGG152090.18978201833781697No Hit
TGAAAATCCTTGCTCTGAAGTGGAA148750.18561427594023458No Hit
TTGTTTATGCCGTGAGGATTGGAGGAAAA142620.1779650960308992No Hit
CCAACTCCGACAGCATAGGTGTGCGATGT142190.17742853039288708No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAA137960.17215022190732612No Hit
CGAGTAGCGCTTAGTGAATT135240.16875613229013325No Hit
TGAAAATCTTTGCTCTGAGGTGGAA130500.16284143200134865No Hit
TGAAAATCTTTGCTCTGAAGTGGTA123470.15406920773338328No Hit
TGAAAATCTTTGCTCTGAAGTGAA122820.1532581201410394No Hit
CACCCCTCTTCCACTAACTTGACCTCATT122650.15304598953996484No Hit
TCTCGGGCTGAGACTTGAAGCGGGT118890.14835415977502178No Hit
TCATACAATGGACAAGGATCAGG117250.1463077233881849No Hit
TGAAAATCTTTGCTCTGGAGTGGAA116200.14499750496978325No Hit
TGAAGATCTTTGCTCTGAAGTGGAA111320.1389081088918784No Hit
CACCCCTCCTCCAAATACTTGACCTCTT111160.1387084565614553No Hit
TGAAAATCTTTGCTCTGAAGT109820.13703636829416177No Hit
CCAACTCCGACAGCATAGGTGTGCGATG105540.13169566845534358No Hit
TGAAAATCTTTGCCCTGAAGTGGAA104780.1307473198858338No Hit
TGGAAATCTTTGCTCTGAAGTGGAA104480.13037297176629048No Hit
TAGTGATAGCGTCTTGGCTCAAAAAA101930.12719101275017217No Hit
TGAGAATCTTTGCTCTGAAGTGGAA101360.12647975132303985No Hit
TTGACTGATAAAACTTCTCTACATAAAAGT98980.12350992290799609No Hit
TGAAAATCTTTGCTCCGAAGTGGAA93820.11707113525185081No Hit
GAGGTCCGGATTTGGGGAGGGTTATCTACT92890.11591065608126648No Hit
CGAAAATCTTTGCTCTGAAGTGGAA91930.11471274209872782No Hit
ATGTACAATTCTAGACTCTTTGGTGGT84940.10599043091336824No Hit
CACCCCTCTTCCACTAACTTGACCTCAT84650.10562856106447634No Hit
TATTAATGCTGTAGGTGAAAGACC84340.10524173467428158No Hit
TATCAGCATCTGTTCAGCATCTGACGTGT84200.10506703888516135No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGCG83390.10405629896239436No Hit
TGATAAACTCGCTCGTACTCACA82100.10244660204835805No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)