FastQCFastQC Report
Mon 24 Feb 2025
POR-83-TP4_R1_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-83-TP4_R1_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23207558
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCAC2291350.9873292140431148No Hit
CTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTC1482960.6389987261908383No Hit
TCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTG1137660.4902109907470661No Hit
TTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTT1069960.46103945964500015No Hit
GAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTGCG1055280.4547139341416275No Hit
TTTGGTTTCCCATAAGGTGCCAAAGGGGTCATCCAATAACACCCTCTGAT866060.3731801510525149No Hit
AAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGC849470.3660316178031312No Hit
GCACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCA784350.3379717934993419No Hit
TAATCATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTAT746350.3215978174006933No Hit
AGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAG725710.31270416301448No Hit
CAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCC690840.2976788854734307No Hit
ATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGC688070.2964853087946608No Hit
AATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAG683370.2944601064877227No Hit
TCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCC680310.29314157051767364No Hit
AGATTTCTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATTTG612640.2639829662388434No Hit
CTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGC598040.2576919122640995No Hit
TTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGG595860.2567525631089665No Hit
CTAATTTTTTCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTTAAGA573340.24704882779997794No Hit
GTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCCAATCC566480.24409289422006403No Hit
TCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGC526950.22705965013639093No Hit
CTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCA521200.22458200901620068No Hit
GATTACCCACACCTCTCGGTGAAGGAGACACTCTCTGACATCGTCAGTGT512490.2208289213367473No Hit
CACTCTAATTTTTTCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTT501060.21590380168391693No Hit
GTTAAAGGATTTAAGTTGTACTCATTCCAATTACAAGACTTGTAAAAGCC483660.20840624420716733No Hit
ACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCT482280.2078116103383217No Hit
ATCATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTATTC482200.20777713880969295No Hit
AAAATTGCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCA477650.20581657061893374No Hit
ACTTCCCCCGGAACCCAAAAACTTTGGTTTCCCATAAGGTGCCAAAGGGG465720.20067600391217377No Hit
CATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTG425730.18344454853888548No Hit
TCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGT422160.18190625657382825No Hit
CAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTG419810.18089365542035918No Hit
AAATTGCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCAC418680.18040674507847831No Hit
CTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAG405240.17461552826885102No Hit
GATTTCTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATTTGA404970.17449918685972907No Hit
ACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACT404520.17430528451119243No Hit
CAACCATGGTAAGCCACTACCTTACCATCGACAGTTGATAGGGCAGAAAT386620.16659227998051326No Hit
GCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACC383790.1653728496552718No Hit
CCGAAGACGAGAGTTAAGTCCAGAACGTGGACCTTGCGGTCCGCTTCCAC382740.16492041084201964No Hit
TAATCAGCGCGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGTTTAAG375250.16169301397415445No Hit
CTACATTGTTCTATCAACCAGAGGCTGCTCACCTCGGAGACCTGCTGCGG370850.1597970798995741No Hit
GATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTAATTC360940.1555269192906897No Hit
AGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTATTCAGGCATAGTTCAC359210.15478147248409332No Hit
GGCAGGGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAGGAATTC356610.1536611478036595No Hit
CCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGACAGTTCGATTAGTCT351210.15133431962121996No Hit
TTTAAAAGATTACCCACACCTCTCGGTGAAGGAGACACTCTCTGACATCG350190.15089480763120358No Hit
TTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTCCGGTTCG349010.15038635258392977No Hit
CTGCGTTATCATTTGACAGATGTGCCGCCCCAGCCAAACTCCCAACCTGA345650.14893854838152296No Hit
GTCCAAGAGAAGTGCGACGGTCGGAAGCGACGGGCCCGAGACCGCGGTCC341910.14732700441812965No Hit
GCACTTTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGTTCTGCTTACC339790.1464135089094682No Hit
GTTTCCTCTGGCTTCACCCTATTCAGGCATAGTTCACCATCTTTCGGGTC339660.1463574926754465No Hit
GTCTTCTATTCTTGTTCGCCTAAGGTTATCTGGAGGTAATTATCGTTGCT339620.14634025691113214No Hit
GAACATCTAAGGGCATCACAGACCTGTTATTGCCTTCCTGACTTTGGTTG339180.14615066350367412No Hit
AGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCCC326480.1406783083338626No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT325100.14008367446501696No Hit
ACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGTTT324510.13982944694138003No Hit
CCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGCGAGCGTCCGG323560.13942009753891382No Hit
GGTGGGTGAACAATCCATCACTTGGCGAATTCTGCTTCGCAATGATAGGA323400.13935115448165636No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC318690.13732164323363966No Hit
ATGACCGCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGAC312840.1348009127026635No Hit
GCCTTCCTGACTTTGGTTGGATACCAACAGTCCCTCTAAGAAGTTTACCA312070.13446912423961194No Hit
TACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAGC311810.13435709177156857No Hit
TTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAAAA307480.13249132028453833No Hit
TGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTTCCCC305580.1316726214796059No Hit
CTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATA295370.12727319263836376No Hit
ACCCGACTCTTCGAAAGCACATCGTGAGCGGCCGGCCGAGCCACAGACGG291120.12544189267996228No Hit
GTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGC290880.12533847809407608No Hit
CCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTC288710.12440343788002167No Hit
CAGAACATCTAAGGGCATCACAGACCTGTTATTGCCTTCCTGACTTTGGT287020.12367522683773967No Hit
AATTCTCCGTCACCCGTTGCAACCATGGTAAGCCACTACCTTACCATCGA286180.12331327578713797No Hit
TGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCGACATCGAAGGATCAA283910.12233514616229765No Hit
TGACGGTGTCTTGGGCTTGGATCCTCCCGTCCGAAGACGAGAGTTAAGTC279840.12058140714331081No Hit
TTCTAGCACGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAACAGATG277060.11938352152346232No Hit
CGTTGACTCGCGTCCAAGAGAAGTGCGACGGTCGGAAGCGACGGGCCCGA276570.11917238341061132No Hit
ATACTTCCCCCGGAACCCAAAAACTTTGGTTTCCCATAAGGTGCCAAAGG275190.11857774954176566No Hit
TACTTCCCCCGGAACCCAAAAACTTTGGTTTCCCATAAGGTGCCAAAGGG273710.11794002626213408No Hit
CCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCAT271680.11706531122317997No Hit
GCCTGACACCCACCCCGACGGCGAAGTATAAGTGCGACGCTTGAGCGCCA270950.11675075852444278No Hit
AATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTA268280.11560027125645878No Hit
TTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGTTCTGCT268150.11554425502243709No Hit
ATAGAACCAAAGTCCTATTCCATTATTCCATGCTTATGTATTCAAGCGCT263060.11335100401343388No Hit
TTACCTGATAAAACTGTACACTGAGTTCCAGCTATCCTGAGGGAAACTTC261230.1125624677960516No Hit
AAGAGGCATGTGCTGCATCCGCCTCGGTCGGGCCCGAGCCGCGAGAGAAC260430.11221775250976426No Hit
TTTCAGCTTTGCAACCATACTTCCCCCGGAACCCAAAAACTTTGGTTTCC258840.1115326308782682No Hit
ACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGTTCTG253340.10916271328504275No Hit
TGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCA252150.10864994929669033No Hit
ATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAGT250890.10810702272078777No Hit
CATCGTTTATGGTTAGAACTAGGACGGTATCTGATCGTCTTCGATCCTCT250730.1080380796635303No Hit
CTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTA250470.10792604719548692No Hit
GGCATCGTTTATGGTTAGAACTAGGACGGTATCTGATCGTCTTCGATCCT249530.10752100673409928No Hit
GAAGTCTTCTATTCTTGTTCGCCTAAGGTTATCTGGAGGTAATTATCGTT249150.1073572669731128No Hit
TTAATCATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTA249020.10730125073909111No Hit
AAGGGCAGGGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAGGAA245700.10587068230099868No Hit
ATTTGACGATCGATTTGCACGTCAGAATCGCTACGAGCCTCCACCAGAGT245190.1056509263059905No Hit
GTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGCGAGC244790.10547856866284681No Hit
CATCGCCAGTTCTGCTTACCAAAAATGGCCCACTAGGCACTCTCATTCGT240880.10379377270111746No Hit
CAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAGTTGTACTCAT240640.10369035811523125No Hit
GACGATCGATTTGCACGTCAGAATCGCTACGAGCCTCCACCAGAGTTTCC239410.10316035836256446No Hit
TACTCAAACCTTCCTAAGAGTAGAATAGGTCGGTCAACGATGCGTCCGGC238850.10291905766216333No Hit
TAGATGGAGTTTACCACCATTTTTGGGCTGCATTCCCGAACAACCCGACT238230.10265190331529064No Hit
CAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCAGAG238150.10261743178666193No Hit
CTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTGGTGGGTGAACAAT237320.1022597896771388No Hit
TTGCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCACACC235940.10166515580829313No Hit
CGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTCCGGTT234940.10123426170043397No Hit
AACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCA232700.10026905889882942No Hit
GAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAGCAGT232460.10016564431294323No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)