FastQCFastQC Report
Mon 24 Feb 2025
POR-74-TP4_R1_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-74-TP4_R1_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19340555
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCAC2086351.078743603790067No Hit
CTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTC1472600.7614052440584047No Hit
GAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTGCG1029260.5321770755803026No Hit
TCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTG985310.5094528052581738No Hit
AAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGC951250.491842142068829No Hit
TTTGGTTTCCCATAAGGTGCCAAAGGGGTCATCCAATAACACCCTCTGAT923930.4777163840437878No Hit
TTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTT868030.44881338720631336No Hit
CAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCC700630.3622595111670787No Hit
ATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGC686180.35478816404182817No Hit
AATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAG658930.3406985993938644No Hit
GCACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCA639490.3306471815312435No Hit
GTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCCAATCC626600.3239824296665737No Hit
TCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCC609710.3152494848260559No Hit
AGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAG590800.305472102532735No Hit
TAATCATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTAT582070.3009582713629469No Hit
TTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGG549850.28429897694249207No Hit
GATTACCCACACCTCTCGGTGAAGGAGACACTCTCTGACATCGTCAGTGT549440.28408698716246766No Hit
CTAATTTTTTCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTTAAGA533120.2756487598210082No Hit
CTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGC528930.27348232767880754No Hit
TCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGT503430.2602975974577772No Hit
TCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGC502770.2599563456167623No Hit
ACTTCCCCCGGAACCCAAAAACTTTGGTTTCCCATAAGGTGCCAAAGGGG488610.2526349424822607No Hit
AGATTTCTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATTTG475130.2456651321536533No Hit
CTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCA474000.24508086763797626No Hit
GTTAAAGGATTTAAGTTGTACTCATTCCAATTACAAGACTTGTAAAAGCC456360.23596013661448703No Hit
ATCATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTATTC448370.23182892114523082No Hit
ACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCT411770.21290495541622254No Hit
GCACTTTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGTTCTGCTTACC407420.21065579555498795No Hit
GATTTCTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATTTGA407310.21059892024815216No Hit
CTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAG400590.20712435604872767No Hit
TAATCAGCGCGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGTTTAAG400160.206902025303824No Hit
CACTCTAATTTTTTCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTT393070.2032361532541336No Hit
CCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGACAGTTCGATTAGTCT380930.19695918757243522No Hit
GTCCAAGAGAAGTGCGACGGTCGGAAGCGACGGGCCCGAGACCGCGGTCC375310.1940533764413689No Hit
CCGAAGACGAGAGTTAAGTCCAGAACGTGGACCTTGCGGTCCGCTTCCAC367030.18977221698136376No Hit
CAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTG364930.18868641566904362No Hit
GTTTCCTCTGGCTTCACCCTATTCAGGCATAGTTCACCATCTTTCGGGTC364370.18839686865242491No Hit
CATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTG360000.18613736782631107No Hit
CTACATTGTTCTATCAACCAGAGGCTGCTCACCTCGGAGACCTGCTGCGG357530.184860258663725No Hit
AGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCCC339720.17565162943876222No Hit
AAAATTGCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCA338570.17505702395820597No Hit
CAACCATGGTAAGCCACTACCTTACCATCGACAGTTGATAGGGCAGAAAT338510.17502600106356825No Hit
AAATTGCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCAC337590.1745503166791232No Hit
TGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCGACATCGAAGGATCAA332770.1720581441432265No Hit
TTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTCCGGTTCG329900.1705742156830556No Hit
GCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACC329840.1705431927884179No Hit
GTCTTCTATTCTTGTTCGCCTAAGGTTATCTGGAGGTAATTATCGTTGCT329180.170201940947403No Hit
TTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAAAA328980.1700985312986106No Hit
CTGCGTTATCATTTGACAGATGTGCCGCCCCAGCCAAACTCCCAACCTGA326630.16888346792529998No Hit
ACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACT326240.1686818191101548No Hit
GATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTAATTC305570.15799443190746076No Hit
GGCAGGGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAGGAATTC302690.15650533296485028No Hit
ATGACCGCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGAC300410.155326462968617No Hit
TACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAGC299930.15507827981151523No Hit
GGTGGGTGAACAATCCATCACTTGGCGAATTCTGCTTCGCAATGATAGGA298410.15429236648069303No Hit
TGACGGTGTCTTGGGCTTGGATCCTCCCGTCCGAAGACGAGAGTTAAGTC293320.15166059091892659No Hit
AGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTATTCAGGCATAGTTCAC286900.14834114119269068No Hit
ATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAGT285810.14777755860677214No Hit
GAACATCTAAGGGCATCACAGACCTGTTATTGCCTTCCTGACTTTGGTTG285410.14757073930918735No Hit
CTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCC285370.1475500573794289No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC284210.146950281416433No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT284150.14691925852179527No Hit
GCCTTCCTGACTTTGGTTGGATACCAACAGTCCCTCTAAGAAGTTTACCA279670.1446028823888456No Hit
CCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTC273380.14135064893432478No Hit
AATTCTCCGTCACCCGTTGCAACCATGGTAAGCCACTACCTTACCATCGA273250.14128343266260973No Hit
ACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCCCTTGG272850.14107661336502494No Hit
ATACTTCCCCCGGAACCCAAAAACTTTGGTTTCCCATAAGGTGCCAAAGG271340.14029587051664236No Hit
TACTTCCCCCGGAACCCAAAAACTTTGGTTTCCCATAAGGTGCCAAAGGG271320.14028552955176313No Hit
TGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTTCCCC265840.1374521051748515No Hit
TTTCAGCTTTGCAACCATACTTCCCCCGGAACCCAAAAACTTTGGTTTCC265470.13726079732458557No Hit
ACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGTTT265040.13703846657968194No Hit
CTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATA255040.13186798414006215No Hit
AATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTA254880.13178525642102826No Hit
CGCGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGTTTAAGATCAAAA254730.13170769918443395No Hit
CGTTGACTCGCGTCCAAGAGAAGTGCGACGGTCGGAAGCGACGGGCCCGA250820.12968604055054261No Hit
GTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGCGAGC248250.12835722656356036No Hit
GCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGCGAGCGTCCGGACCTA245740.1270594354712158No Hit
TGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCA245220.12679057038435557No Hit
TACTCAAACCTTCCTAAGAGTAGAATAGGTCGGTCAACGATGCGTCCGGC242540.1254048810905375No Hit
CAGAACATCTAAGGGCATCACAGACCTGTTATTGCCTTCCTGACTTTGGT239710.12394163456012508No Hit
CCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCAT239490.12382788394645346No Hit
ACCCGACTCTTCGAAAGCACATCGTGAGCGGCCGGCCGAGCCACAGACGG238340.12323327846589717No Hit
GGATTCTGACTTAGAGGCGTTCAGTCATAATCCAACAGATGGTAGCTTCG237730.12291787903708037No Hit
GGCCGGAAGGACGGGACCCTAGCCACAAGAGGCATGTGCTGCATCCGCCT237150.12261799105558244No Hit
TTTAAAAGATTACCCACACCTCTCGGTGAAGGAGACACTCTCTGACATCG235560.12179588434768289No Hit
ATAGAACCAAAGTCCTATTCCATTATTCCATGCTTATGTATTCAAGCGCT234240.12111338066565307No Hit
CAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAGTTGTACTCAT233610.12078764027195704No Hit
ACCGGACACGAAAATCATTGTCTCCCGCGGATTTTCAAGGGCCGTCGAGA233500.12073076496512121No Hit
CCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGCGAGCGTCCGG232830.1203843426416667No Hit
AAGAGGCATGTGCTGCATCCGCCTCGGTCGGGCCCGAGCCGCGAGAGAAC232380.1201516709318838No Hit
GAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAGCAGT231630.11976388474891231No Hit
TAAAACTGTACACTGAGTTCCAGCTATCCTGAGGGAAACTTCGGAGGGAA231070.11947433773229361No Hit
GCCTGACACCCACCCCGACGGCGAAGTATAAGTGCGACGCTTGAGCGCCA227140.11744233813352305No Hit
CAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCAGAG226530.11712693870470625No Hit
TTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGTTCTGCT225970.11683739168808754No Hit
CCCACTTATTCTACACCTCTCATGTCTTTTCACAAGGTCAGACTAGAGTC219920.1137092498121176No Hit
ACAAAGGGCAGGGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAG219520.11350243051453279No Hit
GTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGC219000.11323356542767259No Hit
ATTTGACGATCGATTTGCACGTCAGAATCGCTACGAGCCTCCACCAGAGT218210.11282509731494261No Hit
CTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTA218180.11280958586762375No Hit
AACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCA216280.11182719420409601No Hit
TTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCAGAGGCCGT216210.11179100082701866No Hit
CTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTGGTGGGTGAACAAT216160.11176514841482056No Hit
AACCTTTCTCCACTTCGGTCTTCAAAGTTCTCATTTGAATATTTGCTACT215990.11167725021334703No Hit
TGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATAC215180.11125844113573782No Hit
TCTATTCTTGTTCGCCTAAGGTTATCTGGAGGTAATTATCGTTGCTTTCT212920.11008991210438378No Hit
CATCGCCAGTTCTGCTTACCAAAAATGGCCCACTAGGCACTCTCATTCGT211560.10938672649259548No Hit
TATTCATACTGAAAATCAAAATCAAGTGAGCTTTTACCCTTTTGTTCTAC211530.10937121504527662No Hit
AGCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGCGAGCGTCCGGACCT210750.10896791741498628No Hit
GACGATCGATTTGCACGTCAGAATCGCTACGAGCCTCCACCAGAGTTTCC209340.10823887939099991No Hit
TTCTAGCACGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAACAGATG208900.10801137816365662No Hit
CGTCGTCCGGCTTCAATTAAGCAAGTCGGACATCTTACCAATTTAAAGTT208860.10799069623389816No Hit
TACATTGTTCTATCAACCAGAGGCTGCTCACCTCGGAGACCTGCTGCGGT208810.10796484382170006No Hit
TTACCTGATAAAACTGTACACTGAGTTCCAGCTATCCTGAGGGAAACTTC206890.10697211119329306No Hit
CTATAGCAACCAACACCTTTTGTGGGGTCTGATGAGCGTCGACTTTGGCA206510.10677563286058751No Hit
CCTGATAAAACTGTACACTGAGTTCCAGCTATCCTGAGGGAAACTTCGGA205790.10640335812493489No Hit
TAGATGGAGTTTACCACCATTTTTGGGCTGCATTCCCGAACAACCCGACT204850.10591733277561062No Hit
AAGGGCAGGGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAGGAA203950.10545198935604486No Hit
CTATTTTGCCGACTTCCCTTACCTACATTGTTCTATCAACCAGAGGCTGC200040.10343033072215352No Hit
GAAGTCTTCTATTCTTGTTCGCCTAAGGTTATCTGGAGGTAATTATCGTT196270.10148105884241689No Hit
TTGCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCACACC195570.1011191250716435No Hit
CAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCC194710.10067446358183618No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)