FastQCFastQC Report
Mon 24 Feb 2025
POR-74-TP2_R2_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-74-TP2_R2_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17719989
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTC1840651.0387421798060936No Hit
GAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCT1119930.6320150650206385No Hit
GACGAACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAA913370.5154461438999764No Hit
ATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTAACGGCG902530.5093287586126606No Hit
GAACTTTGAAGACCGAAGTGGAGAAAGGTTCCATGTGAACAGCAGTTGGA804770.45415942413959737No Hit
GCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAG763110.43064925153170247No Hit
CAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCT622550.35132640319359115No Hit
TTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTAACGGCGA591440.3337699588865433No Hit
AATGCGGGTTCTGCCCGGTGTCTTTGGTGATTCATAGTAACTGATCGAAT577650.3259877870127346No Hit
CTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAG560020.3160385709043047No Hit
TAACGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGACGCTTG535940.3024493976830347No Hit
TGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGT509930.28777105900009303No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGC501450.28298550298197134No Hit
TGAATTTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCC487660.27520333110816264No Hit
GATTCCCTCAGTAACGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATC484010.2731435104164004No Hit
AAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGCGCC474410.2677258998298475No Hit
GAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAG461860.26064350265680186No Hit
AATTTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT443520.25029360909874154No Hit
GTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGA437070.24665365198590136No Hit
AGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAA424190.23938502444894294No Hit
GAATAGCTCAAATTTGAAATCTCCGACGCTTGCGTCGGCGAATTGTAATT420730.2374324273000395No Hit
CCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAA417050.23535567657519424No Hit
CTTGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGCGGATCTGAA414020.2336457432338135No Hit
AGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTC398700.22500013967277294No Hit
GAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCA381850.2154911044245005No Hit
TTGGCATTTGCACTTGCCTGAAAAGACAATGGTGCGAAGCTACCATCTGT376650.21255656535678438No Hit
TTTGAAATCTCCGACGCTTGCGTCGGCGAATTGTAATTGCGAGAAGCACT367900.20761863904091588No Hit
AAAATGGTGGTAAACTCCATCTAAAGCTAAATACTGACGTGAGACCGATA362470.20455430305289696No Hit
GATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAG361940.20425520580176432No Hit
ATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTA356710.20130373670096524No Hit
CCAAAGTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTATAGAC330040.1862529372901981No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTG324350.18304187434879332No Hit
TGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGCGGATCTGAATG316100.17838611525097448No Hit
TCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAA312340.17626421777124127No Hit
ATAGGGTGAAGCCAGAGGAAACTCTGGTGGAGGCTCGTAGCGATTCTGAC294780.16635450507333838No Hit
GCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACT287080.1620091299153741No Hit
TCAACTGTCGATGGTAAGGTAGTGGCTTACCATGGTTGCAACGGGTGACG286460.16165924256499256No Hit
GGTTCTGCCCGGTGTCTTTGGTGATTCATAGTAACTGATCGAATCGCACG285010.16084095763264863No Hit
GCCGCTGAGGCGAGATTCCGACTCTCGTAGCCGGACGCATCGTTGACCGA284860.16075630746723377No Hit
CGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA274470.1548928726761625No Hit
AATAGCTCAAATTTGAAATCTCCGACGCTTGCGTCGGCGAATTGTAATTG273670.15444140512728308No Hit
AGTGCCAAAGTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTAT272200.1536118335062172No Hit
CTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAACTCAGT271250.1530757157919229No Hit
CTGATGAAGTGTTGTTGCAATAGTAATTCTGCTCAGTACGAGAGGAACCG267480.15094817496782872No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAA266260.15025968695578762No Hit
GTTCTGCCCGGTGTCTTTGGTGATTCATAGTAACTGATCGAATCGCACGG265860.1500339531813479No Hit
AGGCGAGATTCCGACTCTCGTAGCCGGACGCATCGTTGACCGACCTATTC262450.14810957275424946No Hit
GATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGG260900.1472348543782956No Hit
TGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGC255390.14412537163538872No Hit
CTTGCGCTGGCGATGTTTCATTCAAATTTCTGCCCTATCAACTGTCGATG252320.142392864916564No Hit
TAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGC250320.1412641960443655No Hit
AGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGT249630.140874805283457No Hit
TGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTAC244280.137855616050326No Hit
CTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGG242460.13682852737662535No Hit
TTACGAAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTA237140.1338262681765773No Hit
GATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAATA232610.13126983318104768No Hit
TGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTG231850.13084093900961224No Hit
GACAGGTTAGTTTTACCCTACTGATGAAGTGTTGTTGCAATAGTAATTCT231770.1307957922547243No Hit
TACTTATTGGATGAAGTGGAAGCGGACCGCAAGGTCCACGTTCTGGACTT228520.12896170533740173No Hit
CAGTTTTATCAGGTAAAGCGAATGATTAGAGGCATTAGGGTTAAAACAAC227400.12832965076897057No Hit
AAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAAACTGCGAATGG223120.12591429938246576No Hit
TTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAA222980.12583529256141188No Hit
AAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGCG222180.12538382501253245No Hit
GAACTGGCAGTGACTAGGGGAATCCGACTGTTTAATTAAAACAAAGCATT216120.12196395832977096No Hit
ATTTGAAATCTCCGACGCTTGCGTCGGCGAATTGTAATTGCGAGAAGCAC214260.12091429627862635No Hit
GTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATTTCGGGATG214240.12090300958990438No Hit
TTTACAAGTCTTGTAATTGGAATGAGTACAACTTAAATCCTTTAACGAGG213850.12068291915982565No Hit
TGGGTCTCTCGGGCTGAGACTTGAAGCGGGTGGAGCCGGCCTGGGCTGGC213830.12067163247110367No Hit
GAACGAGACCTTAACCTGCTAAATAGTTACGCGAATCTCGATTCGTGGTA213680.12058698230568879No Hit
CTGCCCTATCAACTGTCGATGGTAAGGTAGTGGCTTACCATGGTTGCAAC212000.11963890045304205No Hit
GGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGCGACGTTGCTTTTTG211990.11963325710868104No Hit
GCGCCCCTTGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGCGGA211880.11957118032071012No Hit
ACCTATTCTCAAACTTTAAATTGGTAAGATGTCCGACTTGCTTAATTGAA208380.11759600979436274No Hit
AAACTTTAAATTGGTAAGATGTCCGACTTGCTTAATTGAAGCCGGACGAC204160.11521451847402388No Hit
AGGAGGGCGCAGTGGTCGTGACGCAGCCTTTGGCGCGAGCCTGGGTGAAA200760.11329578139128642No Hit
ATGCGCCCCTTGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGCG200320.11304747423940273No Hit
TATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTAACGGC200030.11288381725293396No Hit
GGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTAT199270.11245492308149853No Hit
GCGAATGATTAGAGGCATTAGGGTTAAAACAACCTTAACCTATTCTCAAA198540.11204295894314607No Hit
GAGGCGAGATTCCGACTCTCGTAGCCGGACGCATCGTTGACCGACCTATT197860.11165921152659858No Hit
TGCGAAAAGTCCCGACTTCTGGAAGGGATGTATTTATTAGATTAAAAACC196900.11111745046794329No Hit
AATTCCGTTAACGAACGAGACCTTAACCTGCTAAATAGTTACGCGAATCT195100.11010164848296462No Hit
TTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCC193220.10904069974309805No Hit
TTGAAGCCGGACGACGAATGAGAGTGCCTAGTGGGCCATTTTTGGTAAGC190550.10753392679871303No Hit
TCGCAAAGACTGTGTGTGCTCTTAACTGAGTGTGCTCAGGATCTGTGACG188070.10613437739718688No Hit
AAGGTAGGTCCGGACGCTCGCGTTCGGACTGTTACAGCCCGGTTCTCTCG186450.10522015561070608No Hit
GCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTTAGAGGATCG183990.1038318928979019No Hit
AGGGAAACGAATGGACTCAGCAATGCGCCCCTTGAGATTCAGCCGGGCGG183470.1035384389911303No Hit
GGCTCGTAGCGATTCTGACGTGCAAATCGATCGTCAAATTTGGGTATAGG183240.10340864207082746No Hit
TAATTGGAATGAGTACAACTTAAATCCTTTAACGAGGATCCATTGGAGGG182760.10313776154149983No Hit
GGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATG182320.10288945438961616No Hit
GGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGCGACGTTGCTTTTTGA181550.10245491687381973No Hit
GTTGGTTTCTGGAACCTTAGTAATGATTAAGAGAGACAGTTGGGGGCATT180480.10185107902719352No Hit
GTCTAAGTATAAGCACTAGTACTGTGAAACTGCGAATGGCTCATTAAATC180430.10182286230538855No Hit
ACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTC179830.101484261643729No Hit
GGAGTGTGTAACAACTCACCTGCCGAAGCAACTAGCCCTGAAAATGGATG179470.10128110124673328No Hit
GTAACTTCGGGAAAAGGATTGGCTCTAAGCGTTGGGTCTCTCGGGCTGAG179370.10122466780312335No Hit
TTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGT177790.10033301939408654No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)