FastQCFastQC Report
Mon 24 Feb 2025
POR-73-TP4_R2_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-73-TP4_R2_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18417766
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTC1149110.6239138883619219No Hit
GACGAACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAA1005200.5457773760400692No Hit
TGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGT925060.5022650412650481No Hit
CTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAG829600.45043465097775703No Hit
GAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCT822770.44672627505420576No Hit
ATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTA707180.3839662204417192No Hit
GAACTTTGAAGACCGAAGTGGAGAAAGGTTCCATGTGAACAGCAGTTGGA706700.38370560251444175No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGC683610.37116879430436894No Hit
GTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGA682610.3706258402892077No Hit
AATGCGGGTTCTGCCCGGTGTCTTTGGTGATTCATAGTAACTGATCGAAT607490.3298391346702961No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTG518070.28128818663457883No Hit
GCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAG508110.27588036464357296No Hit
CAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCT497760.2702607905866542No Hit
GCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACT487270.2645652029676129No Hit
CCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAA474300.25752308939097174No Hit
TCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAA468970.2546291444901624No Hit
AGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGT465130.25254420107194325No Hit
GATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAG455820.24748929919079218No Hit
ATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTAACGGCG449480.24404697073467002No Hit
TGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTAC424400.23042968403442632No Hit
TGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGC388240.21079646684619624No Hit
AATTCCGTTAACGAACGAGACCTTAACCTGCTAAATAGTTACGCGAATCT377140.20476967727790654No Hit
TGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTG362930.1970543007224655No Hit
GTCTAAGTATAAGCACTAGTACTGTGAAACTGCGAATGGCTCATTAAATC354290.19236317803147243No Hit
GAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCA350590.19035424817537588No Hit
TTGGCATTTGCACTTGCCTGAAAAGACAATGGTGCGAAGCTACCATCTGT331720.18010870590928346No Hit
AGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAA329290.17878932765244165No Hit
TTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATT321540.1745814340349421No Hit
GATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGG318380.17286569934703264No Hit
AAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAAACTGCGAATGG315990.1715680392507973No Hit
TTTGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGCGGATCTGAA306780.16656743277116237No Hit
GATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAATA305700.16598104243478823No Hit
CATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGT296840.16117046986045974No Hit
AGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTC291780.1584231225437439No Hit
TTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTAACGGCGA289730.15731006681266338No Hit
CCATTACTACTTGGATAACCGTAGTAATTCTAGAGCTAATACATGCGAAA289420.1571417510679634No Hit
AAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGCGCC288420.15659879705280216No Hit
TCCGTTAACGAACGAGACCTTAACCTGCTAAATAGTTACGCGAATCTCGA287320.1560015476361248No Hit
TGAATTTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCC286980.15581694327097No Hit
GAATAGCTCAAATTTGAAATCTCCGACGCTTGCGTCGGCGAGTTGTAGTT285350.1549319282262572No Hit
TTTACAAGTCTTGTAATTGGAATGAGTACAACTTAAATCCTTTAACGAGG283030.15367227491108315No Hit
CGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA278840.15139729758755757No Hit
TTACGAAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTA277420.15062630288602863No Hit
TTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGT270240.146727893057171No Hit
TGGGTCTCTCGGGCTGAGACTTGAAGCGGGTGGAGCCGGCCTGGGCTGGC269720.14644555696928716No Hit
ATAGGGTGAAGCCAGAGGAAACTCTGGTGGAGGCTCGTAGCGATTCTGAC269480.14631524800564846No Hit
GATTCCCTCAGTAACGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATC268480.14577229399048722No Hit
CTGATGAAGTGTTGTTGCAATAGTAATTCTGCTCAGTACGAGAGGAACCG265230.14400769344121322No Hit
AAGCACTAGTACTGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTT263610.14312810793665204No Hit
GAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAG263280.14294893311164883No Hit
TCAACTGTCGATGGTAAGGTAGTGGCTTACCATGGTTGCAACGGGTGACG262240.14238426093588114No Hit
CCAAAGTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTATAGAC261910.14220508611087793No Hit
TGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGCGGATCTGAATG260900.1416567025555651No Hit
GAACGAGACCTTAACCTGCTAAATAGTTACGCGAATCTCGATTCGTGGTA258010.14008756545174914No Hit
TAACGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGACGCTTG256670.1393600070714331No Hit
GTATAAGCACTAGTACTGTGAAACTGCGAATGGCTCATTAAATCAGTTAT255460.138703032713088No Hit
GCCGCTGAGGCGAGATTCCGACTCTCGTAGCCGGACGCATCGTTGACCGA255190.1385564351289945No Hit
GGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGCGACGTTGCTTTTTG251220.1364009076888044No Hit
GGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATG250360.13593396723576573No Hit
AGTGCCAAAGTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTAT247540.13440283691301105No Hit
GCATGTCTAAGTATAAGCACTAGTACTGTGAAACTGCGAATGGCTCATTA244540.13277397486752734No Hit
GACAGGTTAGTTTTACCCTACTGATGAAGTGTTGTTGCAATAGTAATTCT239170.12985831180611157No Hit
TAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGC235990.12813171803789883No Hit
GTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTG234710.12743673689849247No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAA232580.12628024484619904No Hit
GTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATTTCGGGATG227400.12346774304766386No Hit
TTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCC219760.11931957437183206No Hit
GCGCCCTTTGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGCGGA219530.11919469494834499No Hit
GACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGT218620.11870060679454826No Hit
CTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAACTCAGT215660.11709346290967103No Hit
AATTTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT209900.11396604778234232No Hit
TTTGAAATCTCCGACGCTTGCGTCGGCGAGTTGTAGTTGCGAGAAGCACT208120.11299958963535534No Hit
ATAAGCACTAGTACTGTGAAACTGCGAATGGCTCATTAAATCAGTTATCG206130.1119191111451845No Hit
AATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATTTC204730.11115897552395877No Hit
AAGCACCACACTTCTTGTGGTGTCCGGTGCGTTCTCGACGGCCCTTGAAA204130.11083320311486203No Hit
CAGTTTTATCAGGTAAAGCGAATGATTAGAGGCATTAGGGTTAAAACAAC201380.10934007957316864No Hit
GGTTCTGCCCGGTGTCTTTGGTGATTCATAGTAACTGATCGAATCGCACG201020.1091446161277106No Hit
TAATTGGAATGAGTACAACTTAAATCCTTTAACGAGGATCCATTGGAGGG200420.10881884371861386No Hit
GTAACTTCGGGAAAAGGATTGGCTCTAAGCGTTGGGTCTCTCGGGCTGAG199670.10841162820724294No Hit
GTTCTGCCCGGTGTCTTTGGTGATTCATAGTAACTGATCGAATCGCACGG197880.10743974052010434No Hit
TTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTG196870.10689135696479149No Hit
AAAATGGTGGTAAACTCCATCTAAAGCTAAATACTGACGTGAGACCGATA194810.10577287169355935No Hit
AAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGCG193990.10532764940112714No Hit
AGGCGAGATTCCGACTCTCGTAGCCGGACGCATCGTTGACCGACCTATTC192570.10455665469959821No Hit
TACTTATTGGATGAAGTGGAAGCGGACCGCAAGGTCCACGTTCTGGACTT191960.10422545275034986No Hit
GCGAATGATTAGAGGCATTAGGGTTAAAACAACCTTAACCTATTCTCAAA191630.10404627792534665No Hit
AGGAGGGCGCAGTGGTCGTGACGCAGCCTTTGGCGCGAGCCTGGGTGAAA191430.10393768712231441No Hit
GCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTTAGAGGATCG191000.10370421689579506No Hit
GAACTGGCAGTGACTAGGGGAATCCGACTGTTTAATTAAAACAAAGCATT188780.10249885898213713No Hit
GTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTG188470.10233054323743715No Hit
ATGCGCCCTTTGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGCG187580.10184731416394367No Hit
TACGAAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTAA186910.10148353497378565No Hit
GGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGCGACGTTGCTTTTTGA186500.10126092382756954No Hit
CCTGACTCAGGGAGGTAGTGACAAGAAATAACAATACAGGGCTTTTACAA185590.10076683567377281No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)