FastQCFastQC Report
Mon 24 Feb 2025
POR-73-TP2_R2_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-73-TP2_R2_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21423162
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTC1703990.7953961231306564No Hit
GAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCT1103370.5150360156918012No Hit
GAACTTTGAAGACCGAAGTGGAGAAAGGTTCCATGTGAACAGCAGTTGGA1072150.5004630035472821No Hit
GACGAACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAA969370.4524868924577988No Hit
TGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGT805560.37602292322673936No Hit
CTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAG780100.36413858981227887No Hit
GCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAG725250.33853545989149497No Hit
CAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCT660280.3082084708130387No Hit
ATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTAACGGCG647110.3020609189250401No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGC631590.29481642345793774No Hit
GTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGA630520.29431696404106916No Hit
ATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTA613350.28630227414608544No Hit
TTGGCATTTGCACTTGCCTGAAAAGACAATGGTGCGAAGCTACCATCTGT551220.2573009530525886No Hit
AATGCGGGTTCTGCCCGGTGTCTTTGGTGATTCATAGTAACTGATCGAAT513900.23988055544741713No Hit
GAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCA471360.2200235427431301No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTG465540.2173068569429667No Hit
TTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTAACGGCGA452330.21114063367489821No Hit
AGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAA450510.21029108588172No Hit
TCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAA442710.20665016676809897No Hit
TTTGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGCGGATCTGAA439780.2052824881779823No Hit
AAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGCGCC432970.2021036857210901No Hit
GCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACT427760.19967173846699193No Hit
TGAATTTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCC402500.1878807619528807No Hit
CCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAA402440.18785275488277595No Hit
CTGATGAAGTGTTGTTGCAATAGTAATTCTGCTCAGTACGAGAGGAACCG393910.1838710830828801No Hit
AGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGT393120.18350232332650054No Hit
GATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAG390290.18218132318655855No Hit
CCAAAGTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTATAGAC385610.1799967717183859No Hit
AGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTC383250.17889516029426467No Hit
TGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTAC382050.17833501889216913No Hit
CGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA381640.17814363724645316No Hit
TAACGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGACGCTTG380690.17770019196979417No Hit
GATTCCCTCAGTAACGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATC374920.1750068453947181No Hit
TGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGCGGATCTGAATG374910.17500217754970065No Hit
GAATAGCTCAAATTTGAAATCTCCGACGCTTGCGTCGGCGAGTTGTAGTT366770.17120255170548587No Hit
ATAGGGTGAAGCCAGAGGAAACTCTGGTGGAGGCTCGTAGCGATTCTGAC364770.17026898270199328No Hit
TGGGTCTCTCGGGCTGAGACTTGAAGCGGGTGGAGCCGGCCTGGGCTGGC361900.16892931118198143No Hit
GACAGGTTAGTTTTACCCTACTGATGAAGTGTTGTTGCAATAGTAATTCT343370.16027979436462272No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAA342640.15993904167834794No Hit
TGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGC338060.15780116866034996No Hit
AGTGCCAAAGTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTAT337450.15751643011428473No Hit
TGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTG330700.15436563472749726No Hit
GGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGCGACGTTGCTTTTTG326040.15219041894935958No Hit
GCCGCTGAGGCGAGATTCCGACTCTCGTAGCCGGACGCATCGTTGACCGA324590.15151358142182744No Hit
AAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAAACTGCGAATGG322680.15062202302349204No Hit
AATTCCGTTAACGAACGAGACCTTAACCTGCTAAATAGTTACGCGAATCT321260.14995918903101232No Hit
GTCTAAGTATAAGCACTAGTACTGTGAAACTGCGAATGGCTCATTAAATC318940.14887624898696092No Hit
AATTTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT315970.14748989901677445No Hit
GATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGG306440.14304144271513236No Hit
TAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGC305920.14279871477422426No Hit
CTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAACTCAGT299450.1397786190479258No Hit
CAGTTTTATCAGGTAAAGCGAATGATTAGAGGCATTAGGGTTAAAACAAC299260.139689929992594No Hit
TACTTATTGGATGAAGTGGAAGCGGACCGCAAGGTCCACGTTCTGGACTT290860.13576894017792518No Hit
TTTGAAATCTCCGACGCTTGCGTCGGCGAGTTGTAGTTGCGAGAAGCACT287440.13417253718195288No Hit
GCGCCCTTTGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGCGGA286570.1337664346654336No Hit
AAAATGGTGGTAAACTCCATCTAAAGCTAAATACTGACGTGAGACCGATA286110.1335517137946303No Hit
GAACTGGCAGTGACTAGGGGAATCCGACTGTTTAATTAAAACAAAGCATT285660.1333416607688445No Hit
GATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAATA285330.13318762188326821No Hit
TTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATT274340.12805766020907652No Hit
GTAACTTCGGGAAAAGGATTGGCTCTAAGCGTTGGGTCTCTCGGGCTGAG269330.1257190698553276No Hit
AAGCACCACACTTCTTGTGGTGTCCGGTGCGTTCTCGACGGCCCTTGAAA267900.12505156801783043No Hit
GCGAATGATTAGAGGCATTAGGGTTAAAACAACCTTAACCTATTCTCAAA266110.12421602375970457No Hit
CCATTACTACTTGGATAACCGTAGTAATTCTAGAGCTAATACATGCGAAA265720.12403397780402352No Hit
AGGAGGGCGCAGTGGTCGTGACGCAGCCTTTGGCGCGAGCCTGGGTGAAA262280.12242823911801629No Hit
AGGCGAGATTCCGACTCTCGTAGCCGGACGCATCGTTGACCGACCTATTC262130.12235822144275434No Hit
TTACGAAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTA256820.11987959573848156No Hit
GAACGAGACCTTAACCTGCTAAATAGTTACGCGAATCTCGATTCGTGGTA253950.11853992421846972No Hit
TTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGT253130.11815716092703776No Hit
TCCGTTAACGAACGAGACCTTAACCTGCTAAATAGTTACGCGAATCTCGA252200.11772305134041372No Hit
ACCTATTCTCAAACTTTAAATTGGTAAGATGTCCGACTTGCTTAATTGAA251800.1175363375397152No Hit
AAACTTTAAATTGGTAAGATGTCCGACTTGCTTAATTGAAGCCGGACGAC249350.11639271551043678No Hit
AAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGCG248370.11593526669872542No Hit
GCTTGACTCTAGTCTGACCTTGTGAAAAGACATGAGAGGTGTAGAATAAG248260.11588392040353333No Hit
GGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGCGACGTTGCTTTTTGA248180.11584657764339362No Hit
CATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGT248120.11581857057328886No Hit
ATGCGCCCTTTGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGCG247190.11538446098666481No Hit
AAGCACTAGTACTGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTT247100.11534245038150763No Hit
GAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAG246100.11487566587976136No Hit
AGACAGGTTAGTTTTACCCTACTGATGAAGTGTTGTTGCAATAGTAATTC243920.11385807566595445No Hit
CTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGG239870.11196759843388197No Hit
TTTACAAGTCTTGTAATTGGAATGAGTACAACTTAAATCCTTTAACGAGG239220.1116641885077469No Hit
GCATGTCTAAGTATAAGCACTAGTACTGTGAAACTGCGAATGGCTCATTA237100.11067460536404476No Hit
TAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGCGTTGGGTCTCTCG232010.10829867225015616No Hit
GTATAAGCACTAGTACTGTGAAACTGCGAATGGCTCATTAAATCAGTTAT228540.10667893002909655No Hit
GGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATG228160.10650155191843295No Hit
TTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCC226490.10572202180051667No Hit
TCAACTGTCGATGGTAAGGTAGTGGCTTACCATGGTTGCAACGGGTGACG224110.10461107468636048No Hit
ACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTC223700.10441969304064451No Hit
AAGGTAGGTCCGGACGCTCGCGTTCGGACTGTTACAGCCCGGTTCTCTCG219100.1022724843326116No Hit
TTGAAGCCGGACGACGAATGAGAGTGCCTAGTGGGCCATTTTTGGTAAGC218350.10192239595630187No Hit
AGCGTTGGGTCTCTCGGGCTGAGACTTGAAGCGGGTGGAGCCGGCCTGGG218210.1018570461260574No Hit
GTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTG216560.10108685169817602No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)