FastQCFastQC Report
Mon 24 Feb 2025
POR-73-TP1_R2_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-73-TP1_R2_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20022255
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTC995720.4973066220563069No Hit
GAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCT983990.49144814108101215No Hit
GAACTTTGAAGACCGAAGTGGAGAAAGGTTCCATGTGAACAGCAGTTGGA788380.39375185262599044No Hit
TGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGT740300.3697385734024464No Hit
GACGAACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAA653910.3265915852135536No Hit
ATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTA626330.3128169129800814No Hit
CAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCT589960.29465212584696376No Hit
CTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAG548610.27400010638162386No Hit
AATGCGGGTTCTGCCCGGTGTCTTTGGTGATTCATAGTAACTGATCGAAT545300.2723469459359098No Hit
GAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCA514760.25709391874191995No Hit
ATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTAACGGCG483370.24141636394102461No Hit
GCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAG476420.23794522644926858No Hit
AGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAA445950.22272716035231796No Hit
AATTCCGTTAACGAACGAGACCTTAACCTGCTAAATAGTTACGCGAATCT427240.21338255855796462No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGC425670.21259843109579815No Hit
GTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGA407730.20363840136887681No Hit
GAATAGCTCAAATTTGAAATCTCCGACGCTTGCGTCGGCGAGTTGTAGTT406530.20303906827677504No Hit
CCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAA400330.19994251396758256No Hit
GATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAG384250.19191145053341893No Hit
AGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGT382640.19110734530151574No Hit
TGGGTCTCTCGGGCTGAGACTTGAAGCGGGTGGAGCCGGCCTGGGCTGGC380470.19002355129329837No Hit
ATAGGGTGAAGCCAGAGGAAACTCTGGTGGAGGCTCGTAGCGATTCTGAC375170.18737649680318227No Hit
GCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACT374870.18722666353015682No Hit
TGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGC372110.18584819741832276No Hit
GCCGCTGAGGCGAGATTCCGACTCTCGTAGCCGGACGCATCGTTGACCGA370370.1849791644347752No Hit
GGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGCGACGTTGCTTTTTG369700.18464453679168505No Hit
TGAATTTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCC366870.18323110958281172No Hit
TGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTAC365010.18230214329005398No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTG356200.1779020395055402No Hit
GATTCCCTCAGTAACGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATC339690.16965621504670678No Hit
CGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA336450.16803801569803203No Hit
TTGGCATTTGCACTTGCCTGAAAAGACAATGGTGCGAAGCTACCATCTGT336200.1679131546371775No Hit
AGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTC331070.16535100566844244No Hit
AGTGCCAAAGTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTAT308200.15392871582146966No Hit
GTCTAAGTATAAGCACTAGTACTGTGAAACTGCGAATGGCTCATTAAATC308110.15388376583956204No Hit
TTTGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGCGGATCTGAA298280.14897422892676176No Hit
TCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAA297780.14872450680505267No Hit
TAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGCGTTGGGTCTCTCG297490.14857966797446143No Hit
CTGATGAAGTGTTGTTGCAATAGTAATTCTGCTCAGTACGAGAGGAACCG291300.14548810810770316No Hit
TTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATT286720.14320065347284808No Hit
TTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTAACGGCGA283560.14162240966364678No Hit
GCTTGACTCTAGTCTGACCTTGTGAAAAGACATGAGAGGTGTAGAATAAG275850.13777169454689295No Hit
CCAAAGTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTATAGAC270540.13511964561434264No Hit
GTAACTTCGGGAAAAGGATTGGCTCTAAGCGTTGGGTCTCTCGGGCTGAG267890.13379611836928457No Hit
AATAGCTCAAATTTGAAATCTCCGACGCTTGCGTCGGCGAGTTGTAGTTG266620.13316182418014355No Hit
CATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGT264710.1322078856752149No Hit
TCCGTTAACGAACGAGACCTTAACCTGCTAAATAGTTACGCGAATCTCGA264330.132018096862716No Hit
AAGCACCACACTTCTTGTGGTGTCCGGTGCGTTCTCGACGGCCCTTGAAA264160.13193319134133494No Hit
GACAGGTTAGTTTTACCCTACTGATGAAGTGTTGTTGCAATAGTAATTCT262620.131164047206471No Hit
AGACAGGTTAGTTTTACCCTACTGATGAAGTGTTGTTGCAATAGTAATTC260730.13022009758641073No Hit
TTTACAAGTCTTGTAATTGGAATGAGTACAACTTAAATCCTTTAACGAGG260580.130145180949898No Hit
TTACGAAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTA259570.12964074226404568No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAA256660.1281873595156989No Hit
TAACGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGACGCTTG255520.12761799307820224No Hit
TGCGCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGA253810.1267639434219572No Hit
TAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGC253760.1267389712097863No Hit
GCGCCCTTTGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGCGGA251380.12555029391045114No Hit
TGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGCGGATCTGAATG250590.12515573295815083No Hit
CGCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACG244840.12228392855849653No Hit
GCGAATGATTAGAGGCATTAGGGTTAAAACAACCTTAACCTATTCTCAAA241690.1207106791917294No Hit
AGCGTTGGGTCTCTCGGGCTGAGACTTGAAGCGGGTGGAGCCGGCCTGGG240610.12017127940883782No Hit
GTATAAGCACTAGTACTGTGAAACTGCGAATGGCTCATTAAATCAGTTAT239710.11972177958976149No Hit
CTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAACTCAGT238780.11925729644338262No Hit
GATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAATA237300.11851811896312378No Hit
CTTCGGCCGCTGAGGCGAGATTCCGACTCTCGTAGCCGGACGCATCGTTG231340.1155414312723517No Hit
ATGCGCCCTTTGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGCG229250.11449759280360777No Hit
GGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGCGACGTTGCTTTTTGA229110.11442767060952926No Hit
AAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGCGCC228240.11399315411775547No Hit
CTTTGAAAAGAGAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAA227900.11382334307499331No Hit
AGGAGGGCGCAGTGGTCGTGACGCAGCCTTTGGCGCGAGCCTGGGTGAAA226390.11306918226743191No Hit
CAGTTTTATCAGGTAAAGCGAATGATTAGAGGCATTAGGGTTAAAACAAC221210.11048206108652597No Hit
GACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGT220890.11032223892863216No Hit
CCATTACTACTTGGATAACCGTAGTAATTCTAGAGCTAATACATGCGAAA220030.10989271687929256No Hit
GCAATGCGCCCTTTGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTT217030.10839438414903815No Hit
TTTGAAATCTCCGACGCTTGCGTCGGCGAGTTGTAGTTGCGAGAAGCACT216850.1083044841852229No Hit
TGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTG216580.1081696342395No Hit
AAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGCG215060.10741047898950443No Hit
GAACTGGCAGTGACTAGGGGAATCCGACTGTTTAATTAAAACAAAGCATT213620.10669127927898231No Hit
GTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATTTCGGGATG207360.10356475831518477No Hit
CTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGG206910.10334000840564661No Hit
GACGCATCGTTGACCGACCTATTCTACTCTTAGGAAGGTTTGAGTAAGAG203160.10146709249282862No Hit
GGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAA202750.10126232035302717No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)