FastQCFastQC Report
Mon 24 Feb 2025
POR-72-TP1_R2_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-72-TP1_R2_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16114210
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTC494350.3067789236952975No Hit
GAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCT491180.3048117158706508No Hit
GAACTTTGAAGACCGAAGTGGAGAAAGGTTCCATGTGAACAGCAGTTGGA346340.21492831482275584No Hit
GAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCA326860.2028396055406998No Hit
CAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCT309970.1923581733141122No Hit
AGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAA272160.1688944105854398No Hit
GCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAG245100.15210177849239895No Hit
ATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTAACGGCG230520.14305386363960754No Hit
GAATAGCTCAAATTTGAAATCTCCGACGCTTGCGTCGGCGAATTGTAATT196230.12177450833767214No Hit
GACGAACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAA195420.12127184640140595No Hit
GATTCCCTCAGTAACGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATC194230.12053336775429883No Hit
AATGCGGGTTCTGCCCGGTGTCTTTGGTGATTCATAGTAACTGATCGAAT191000.11852892571215096No Hit
TGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGT187870.11658654069917174No Hit
ATAGGGTGAAGCCAGAGGAAACTCTGGTGGAGGCTCGTAGCGATTCTGAC181400.11257145091195908No Hit
GCCGCTGAGGCGAGATTCCGACTCTCGTAGCCGGACGCATCGTTGACCGA180530.11203155475819168No Hit
TGAATTTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCC178730.1109145282331557No Hit
AGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTC169140.1049632591358807No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)