FastQCFastQC Report
Mon 24 Feb 2025
POR-69-TP3_R2_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-69-TP3_R2_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20790481
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTC2127191.0231557413221946No Hit
GAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCT1123730.5405021653900167No Hit
GAACTTTGAAGACCGAAGTGGAGAAAGGTTCCATGTGAACAGCAGTTGGA1111450.5345956161379817No Hit
GACGAACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAA949790.4568388773689267No Hit
CTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAG878900.4227415421509488No Hit
GCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAG877870.4222461231175941No Hit
ATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTAACGGCG716260.3445134338161777No Hit
TGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGT712820.3428588304426434No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGC687300.3305839821599125No Hit
GTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGA683650.3288283710222962No Hit
CAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCT638340.3070347434482156No Hit
AAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGCGCC577770.2779012183508405No Hit
TTGGCATTTGCACTTGCCTGAAAAGACAATGGTGCGAAGCTACCATCTGT574680.27641496125077625No Hit
TCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAA503090.24198093348585825No Hit
CTTGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGCGGATCTGAA499090.24005697607477192No Hit
TTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTAACGGCGA477940.22988405126365283No Hit
AGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAA457810.22020173559236075No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTG447250.2151224880270928No Hit
TAACGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGACGCTTG440840.2120393462758269No Hit
CCAAAGTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTATAGAC440180.21172189330299768No Hit
AGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTC437560.21046170119873608No Hit
ATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTA435610.2095237719608315No Hit
AATGCGGGTTCTGCCCGGTGTCTTTGGTGATTCATAGTAACTGATCGAAT421960.20295826729549932No Hit
GATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGG397930.19140009314839806No Hit
CTGATGAAGTGTTGTTGCAATAGTAATTCTGCTCAGTACGAGAGGAACCG392480.1887787011757929No Hit
AAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAAACTGCGAATGG388900.18705675929287063No Hit
TGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGCGGATCTGAATG383710.18456042455198607No Hit
GAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCA383330.18437764859793287No Hit
CCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAA382030.1837523624393298No Hit
GATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAG379510.18254026927034542No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAA374270.1800198850618223No Hit
GCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACT374240.18000545538123913No Hit
CGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA367500.17676358714355864No Hit
GATTCCCTCAGTAACGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATC352870.1697267129125103No Hit
AATTTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT344550.16572488149745068No Hit
CTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAACTCAGT344200.16555653522398062No Hit
TGAATTTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCC338500.16281489591318257No Hit
TACTTATTGGATGAAGTGGAAGCGGACCGCAAGGTCCACGTTCTGGACTT336570.1618865864623334No Hit
TGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTG334070.16068411308040445No Hit
AAAATGGTGGTAAACTCCATCTAAAGCTAAATACTGACGTGAGACCGATA330800.15911127789684135No Hit
AGTGCCAAAGTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTAT322420.15508058712061545No Hit
ATAGGGTGAAGCCAGAGGAAACTCTGGTGGAGGCTCGTAGCGATTCTGAC320280.15405126990568424No Hit
GAACGAGACCTTAACCTGCTAAATAGTTACGCGAATCTCGATTCGTGGTA319030.15345003321471976No Hit
AGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGT318930.1534019342794426No Hit
GACAGGTTAGTTTTACCCTACTGATGAAGTGTTGTTGCAATAGTAATTCT313870.15096812815441837No Hit
CAGTTTTATCAGGTAAAGCGAATGATTAGAGGCATTAGGGTTAAAACAAC309890.14905379053038742No Hit
GAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAG308500.14838521533003493No Hit
TTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCC307920.14810624150542742No Hit
TGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTAC307750.14802447331545623No Hit
AGGCGAGATTCCGACTCTCGTAGCCGGACGCATCGTTGACCGACCTATTC302130.1453213131528799No Hit
TGGGTCTCTCGGGCTGAGACTTGAAGCGGGTGGAGCCGGCCTGGGCTGGC302090.14530207357876906No Hit
ACCTATTCTCAAACTTTAAATTGGTAAGATGTCCGACTTGCTTAATTGAA294370.1415888357753724No Hit
TGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGC287860.13845759508882935No Hit
GATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAATA281060.13518686748998254No Hit
CTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGG275110.13232498084099162No Hit
AGGAGGGCGCAGTGGTCGTGACGCAGCCTTTGGCGCGAGCCTGGGTGAAA274940.13224321265102043No Hit
GCCGCTGAGGCGAGATTCCGACTCTCGTAGCCGGACGCATCGTTGACCGA274640.13209891584518896No Hit
GAACTGGCAGTGACTAGGGGAATCCGACTGTTTAATTAAAACAAAGCATT272060.13085796331503827No Hit
GTCTAAGTATAAGCACTAGTACTGTGAAACTGCGAATGGCTCATTAAATC266670.12826543070359941No Hit
TTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGT266110.12799607666604731No Hit
GCGCCCCTTGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGCGGA264420.12718320465986332No Hit
AAGCACTAGTACTGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTT258040.1241144925891806No Hit
AAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGCG255430.12285911037844675No Hit
GGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATG254790.12255127719267293No Hit
AAGGTAGGTCCGGACGCTCGCGTTCGGACTGTTACAGCCCGGTTCTCTCG254160.12224825390042683No Hit
GCGAATGATTAGAGGCATTAGGGTTAAAACAACCTTAACCTATTCTCAAA251160.12080528584211207No Hit
TAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGC249180.11985292692362431No Hit
CCATTACTACTTGGATAACCGTAGTAATTCTAGAGCTAATACATGCGAAA244640.11766923526204132No Hit
AAACTTTAAATTGGTAAGATGTCCGACTTGCTTAATTGAAGCCGGACGAC244470.11758746707207016No Hit
GGTTCTGCCCGGTGTCTTTGGTGATTCATAGTAACTGATCGAATCGCACG244220.11746721973387726No Hit
TCAACTGTCGATGGTAAGGTAGTGGCTTACCATGGTTGCAACGGGTGACG241130.11598096263381304No Hit
GGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGCGACGTTGCTTTTTG240900.11587033508267558No Hit
GTAACTTCGGGAAAAGGATTGGCTCTAAGCGTTGGGTCTCTCGGGCTGAG240840.1158414757215093No Hit
GTTCTGCCCGGTGTCTTTGGTGATTCATAGTAACTGATCGAATCGCACGG239400.1151488510535182No Hit
AAGCACCACACTTCTTGTGGTGTCCGGTGCGTTCTCGACGGCCCTTGAAA238380.11465824191369117No Hit
TTACGAAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTA237610.11428788011205704No Hit
AATTCCGTTAACGAACGAGACCTTAACCTGCTAAATAGTTACGCGAATCT236450.113729932462842No Hit
GCATGTCTAAGTATAAGCACTAGTACTGTGAAACTGCGAATGGCTCATTA235960.11349424767998394No Hit
ACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTC235420.11323451342948726No Hit
ATGCGCCCCTTGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGCG230720.11097386347146079No Hit
GAATAGCTCAAATTTGAAATCTCCGACGCTTGCGTCGGCGAGTTGTAGTT230550.11089209528148965No Hit
GGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGCGACGTTGCTTTTTGA228840.11006960348825022No Hit
CAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAAACT225320.10837652096649424No Hit
CCGGAGATAGGGTTTAATGCCTGGTAAAGCACCACACTTCTTGTGGTGTC224800.108126406503053No Hit
GGAGTGTGTAACAACTCACCTGCCGAAGCAACTAGCCCTGAAAATGGATG224570.10801577895191554No Hit
TAATTGGAATGAGTACAACTTAAATCCTTTAACGAGGATCCATTGGAGGG221980.10677001652823713No Hit
GAAATACCACTACTCTTATCGTTTTTTTACTTATTGGATGAAGTGGAAGC221520.1065487614259622No Hit
GAGGCGAGATTCCGACTCTCGTAGCCGGACGCATCGTTGACCGACCTATT216950.10435064008379605No Hit
TTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATT216820.10428811146793575No Hit
GTAGCGATTCTGACGTGCAAATCGATCGTCAAATTTGGGTATAGGGGCGA216740.104249632319714No Hit
GTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTG215580.10369168467049897No Hit
GGCTCGTAGCGATTCTGACGTGCAAATCGATCGTCAAATTTGGGTATAGG214820.10332613276239255No Hit
GCCAGAGGAAACTCTGGTGGAGGCTCGTAGCGATTCTGACGTGCAAATCG214620.10322993489183824No Hit
TTGAAGCCGGACGACGAATGAGAGTGCCTAGTGGGCCATTTTTGGTAAGC213870.10286919287725955No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)