FastQCFastQC Report
Mon 24 Feb 2025
POR-69-TP1_R2_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-69-TP1_R2_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21802122
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTC1099050.5041023071057029No Hit
GAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCT998960.45819393176499057No Hit
GAACTTTGAAGACCGAAGTGGAGAAAGGTTCCATGTGAACAGCAGTTGGA780560.3580201963827191No Hit
TGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGT780460.3579743292877638No Hit
GACGAACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAA721710.331027411001553No Hit
ATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTA716000.32840839987960807No Hit
CAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCT616800.28290824168399753No Hit
CTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAG598390.2744641095027356No Hit
AATGCGGGTTCTGCCCGGTGTCTTTGGTGATTCATAGTAACTGATCGAAT543550.24931059462927505No Hit
GCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAG512510.2350734483551647No Hit
GAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCA492180.22574866795076184No Hit
ATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTAACGGCG484250.22211140732081033No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGC471670.21634132677543957No Hit
GTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGA467460.21441032207782343No Hit
GCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACT418690.19204093986814677No Hit
AGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGT418500.1919537923877318No Hit
CCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAA416740.19114653151651936No Hit
TGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTAC410460.18826607795332947No Hit
AGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAA403580.18511042182040813No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTG400080.18350507349697429No Hit
AATTCCGTTAACGAACGAGACCTTAACCTGCTAAATAGTTACGCGAATCT393600.18053288574387394No Hit
GATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAG392490.18002376098987063No Hit
TGAATTTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCC384460.17634063326496385No Hit
GATTCCCTCAGTAACGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATC378110.1734280727353053No Hit
GCCGCTGAGGCGAGATTCCGACTCTCGTAGCCGGACGCATCGTTGACCGA374700.1718640047973312No Hit
TGGGTCTCTCGGGCTGAGACTTGAAGCGGGTGGAGCCGGCCTGGGCTGGC367430.16852946699408433No Hit
GGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGCGACGTTGCTTTTTG365290.16754791116204193No Hit
TTGGCATTTGCACTTGCCTGAAAAGACAATGGTGCGAAGCTACCATCTGT361660.1658829356151663No Hit
ATAGGGTGAAGCCAGAGGAAACTCTGGTGGAGGCTCGTAGCGATTCTGAC352640.16174572365020248No Hit
CGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA340160.15602151019978697No Hit
TCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAA330230.1514669076707304No Hit
TGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGC329430.15109997091108837No Hit
AGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTC329200.1509944765926913No Hit
GTCTAAGTATAAGCACTAGTACTGTGAAACTGCGAATGGCTCATTAAATC325410.14925611369388722No Hit
AGTGCCAAAGTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTAT311670.1429539748470355No Hit
CTGATGAAGTGTTGTTGCAATAGTAATTCTGCTCAGTACGAGAGGAACCG308770.1416238290933332No Hit
TTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTAACGGCGA306970.14079822138413867No Hit
TAACGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGACGCTTG294630.1351382218666605No Hit
CTTGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGCGGATCTGAA293350.13455112305123326No Hit
GAATAGCTCAAATTTGAAATCTCCGACGCTTGCGTCGGCGAGTTGTAGTT290720.13334481845391014No Hit
CATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGT283000.12980387872336463No Hit
GCTTGACTCTAGTCTGACCTTGTGAAAAGACATGAGAGGTGTAGAATAAG279010.12797378163465006No Hit
TTACGAAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTA272720.12508874136196468No Hit
TTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATT272150.12482729892071974No Hit
TTTACAAGTCTTGTAATTGGAATGAGTACAACTTAAATCCTTTAACGAGG271040.12431817416671644No Hit
CCAAAGTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTATAGAC268010.1229284011895723No Hit
TAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGCGTTGGGTCTCTCG263760.12097904965397405No Hit
GACAGGTTAGTTTTACCCTACTGATGAAGTGTTGTTGCAATAGTAATTCT260700.11957551654834331No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAA260320.11940122158751337No Hit
TAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGC256420.1176124048842585No Hit
AGACAGGTTAGTTTTACCCTACTGATGAAGTGTTGTTGCAATAGTAATTC254550.1167546902085953No Hit
TCCGTTAACGAACGAGACCTTAACCTGCTAAATAGTTACGCGAATCTCGA253490.1162684990020696No Hit
TGCGCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGA253000.1160437502367889No Hit
GTATAAGCACTAGTACTGTGAAACTGCGAATGGCTCATTAAATCAGTTAT252840.11597036288486047No Hit
CCATTACTACTTGGATAACCGTAGTAATTCTAGAGCTAATACATGCGAAA248990.11420447972908326No Hit
AGGAGGGCGCAGTGGTCGTGACGCAGCCTTTGGCGCGAGCCTGGGTGAAA248220.1138513030979278No Hit
GCGCCCCTTGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGCGGA244870.11231475541692686No Hit
TGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTG244400.11209918007063717No Hit
AAGCACCACACTTCTTGTGGTGTCCGGTGCGTTCTCGACGGCCCTTGAAA243460.11166802937805778No Hit
GTAACTTCGGGAAAAGGATTGGCTCTAAGCGTTGGGTCTCTCGGGCTGAG240440.11028284311040916No Hit
CGCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACG238840.10954896959112512No Hit
AGCGTTGGGTCTCTCGGGCTGAGACTTGAAGCGGGTGGAGCCGGCCTGGG234920.10775097946887921No Hit
AAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGCGCC233750.10721433445790277No Hit
CTTCGGCCGCTGAGGCGAGATTCCGACTCTCGTAGCCGGACGCATCGTTG232190.10649880777660083No Hit
GATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAATA231060.10598050960360647No Hit
GCGAATGATTAGAGGCATTAGGGTTAAAACAACCTTAACCTATTCTCAAA229500.10526498292230455No Hit
GACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGT223780.1026413850908641No Hit
CTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAACTCAGT222990.10227903504071759No Hit
ATGCGCCCCTTGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGCG222800.10219188756030262No Hit
CTTTGAAAAGAGAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAA219900.10086174180660029No Hit
GGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGCGACGTTGCTTTTTGA218640.10028381641016412No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)